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The map label for this gene is cysB [H]

Identifier: 161523997

GI number: 161523997

Start: 894870

End: 895811

Strand: Reverse

Name: cysB [H]

Synonym: Bmul_0818

Alternate gene names: 161523997

Gene position: 895811-894870 (Counterclockwise)

Preceding gene: 161524004

Following gene: 161523996

Centisome position: 25.98

GC content: 63.69

Gene sequence:

>942_bases
ATGAACCTGCACCAATTTCGCTTCGTGCGCGAGGCCGTCCGGCAGAATTTCAACCTCACCGAGGCCGCCAAGGCGCTGTA
CACGTCGCAGCCGGGGGTATCGAAGGCGATCATCGAGCTGGAAGACGAACTGGGCGTCGAGATCTTCACGCGCCACGGCA
AGCGCGTGCGCTCGCTGACCGAGCCCGGCCGCATCATCCTAGCGTCGGTCGAGCGGATCCTGCAGGAAGTCGAAAGCCTG
AAAAGGGTCGGAAAAGATTATGCGGCGCAGGATCAGGGCAACCTGACGATCGCGGCCACGCACACGCAGGCGCGCTACTC
GTTGCCGGCCGCCATCGCCGAGTTCAAGAAGCGCTTCCCGAAGGTGCATCTGTCGATCCTGCAGGGCAGCCCGACGCAGG
TCGCCGAGATGGTGATCCACGACCAGGCCGATCTCGCCGTCGCAACCGAGGCGATCGCCGACTACAAGGAGCTCGTGTCG
CTGCCGTGCTTTCAGTGGCATCACGCGGCCGTCGTGCCGCCCGATCACCCGCTGCTCGAACGCAAGCCCGTCACGCTCGA
CGATCTCGCGCAGTATCCGCTGATCACGTACGACGACGCGTTCGCGGGCCGCAAGAAGATCAATCATGCGTTCGCGCTGC
GCGGCCTGTCCCCGGACATCGTGCTCGAGGCGATCGACGCGGACGTGATCAAGACCTATGTCGAGCTCGGCCTCGGCGTC
GGCATCATGGCCGACATCGCGTTCAATCCCGAGCGCGACCGCAACCTGCGGCTGATTCCGGTCGGCCATCTGTTCGGCAG
CAACGTGACGCGCGTCGCGCTGAAGCAGGGTGCGTATCTGCGCAGCTATGTGTATACGCTTGTCGAATTGCTGTCGCCGA
CGCTGAACCGCAAGCTGATCGAGCAGGCGCTCAAGGGCGAATCCGAATCGTACGAGCTTTGA

Upstream 100 bases:

>100_bases
AAACAGATATCCATATTTGAAAAACTCCGGTGAATCGTAATAAACTCGCCTTATATTTCAAACGACTAAAAAATTCTGTT
GATATGCGGAAGGGTTATAA

Downstream 100 bases:

>100_bases
GCCGCGTGTATAACGACAGGGGCGCATATCGCGCCCCTTTTTGTTGCCCGACGGAGAATCCCGCATGACGCTTCCCGCCC
TGCTGCGCCGCGCCGCCGGC

Product: transcriptional regulator CysB-like protein

Products: NA

Alternate protein names: Cys regulon transcriptional activator [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLTIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLAVATEAIADYKELVS
LPCFQWHHAAVVPPDHPLLERKPVTLDDLAQYPLITYDDAFAGRKKINHAFALRGLSPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNPERDRNLRLIPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPTLNRKLIEQALKGESESYEL

Sequences:

>Translated_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLTIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLAVATEAIADYKELVS
LPCFQWHHAAVVPPDHPLLERKPVTLDDLAQYPLITYDDAFAGRKKINHAFALRGLSPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNPERDRNLRLIPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPTLNRKLIEQALKGESESYEL
>Mature_313_residues
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLTEPGRIILASVERILQEVESL
KRVGKDYAAQDQGNLTIAATHTQARYSLPAAIAEFKKRFPKVHLSILQGSPTQVAEMVIHDQADLAVATEAIADYKELVS
LPCFQWHHAAVVPPDHPLLERKPVTLDDLAQYPLITYDDAFAGRKKINHAFALRGLSPDIVLEAIDADVIKTYVELGLGV
GIMADIAFNPERDRNLRLIPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPTLNRKLIEQALKGESESYEL

Specific function: This protein is a positive regulator of gene expression for the cysteine regulon. The inducer for cysB is N-acetylserine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787530, Length=304, Percent_Identity=45.3947368421053, Blast_Score=296, Evalue=8e-82,
Organism=Escherichia coli, GI1788296, Length=309, Percent_Identity=45.9546925566343, Blast_Score=296, Evalue=9e-82,
Organism=Escherichia coli, GI157672245, Length=213, Percent_Identity=34.2723004694836, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1788481, Length=247, Percent_Identity=27.9352226720648, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI145693105, Length=245, Percent_Identity=26.1224489795918, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=25.0980392156863, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=28.9156626506024, Blast_Score=84, Evalue=8e-18,
Organism=Escherichia coli, GI1789204, Length=242, Percent_Identity=26.8595041322314, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787601, Length=178, Percent_Identity=26.9662921348315, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=264, Percent_Identity=25.7575757575758, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1786401, Length=188, Percent_Identity=27.1276595744681, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI2367136, Length=234, Percent_Identity=21.7948717948718, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87081904, Length=230, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI87082132, Length=147, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1790262, Length=221, Percent_Identity=26.2443438914027, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34944; Mature: 34944

Theoretical pI: Translated: 6.87; Mature: 6.87

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILASVERILQEVESLKRVGKDYAAQDQGNLTIAATHTQARYSLPAAIAEFKKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAEMVIHDQADLAVATEAIADYKELVSLPCFQWHHAAVVPPDHPLLE
CEEEHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
RKPVTLDDLAQYPLITYDDAFAGRKKINHAFALRGLSPDIVLEAIDADVIKTYVELGLGV
CCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMADIAFNPERDRNLRLIPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPTLNRKLI
HHEEEEEECCCCCCCEEEEEEHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHH
EQALKGESESYEL
HHHHCCCCCCCCC
>Mature Secondary Structure
MNLHQFRFVREAVRQNFNLTEAAKALYTSQPGVSKAIIELEDELGVEIFTRHGKRVRSLT
CCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHCHHHHHHCCHHHHHHC
EPGRIILASVERILQEVESLKRVGKDYAAQDQGNLTIAATHTQARYSLPAAIAEFKKRFP
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHCC
KVHLSILQGSPTQVAEMVIHDQADLAVATEAIADYKELVSLPCFQWHHAAVVPPDHPLLE
CEEEHEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCC
RKPVTLDDLAQYPLITYDDAFAGRKKINHAFALRGLSPDIVLEAIDADVIKTYVELGLGV
CCCCCHHHHHCCCEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCC
GIMADIAFNPERDRNLRLIPVGHLFGSNVTRVALKQGAYLRSYVYTLVELLSPTLNRKLI
HHEEEEEECCCCCCCEEEEEEHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHCHHHHHHHH
EQALKGESESYEL
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]