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The map label for this gene is kefA [H]

Identifier: 161523967

GI number: 161523967

Start: 865806

End: 867194

Strand: Direct

Name: kefA [H]

Synonym: Bmul_0787

Alternate gene names: 161523967

Gene position: 865806-867194 (Clockwise)

Preceding gene: 161523966

Following gene: 161523968

Centisome position: 25.11

GC content: 70.48

Gene sequence:

>1389_bases
ATGGAGAACCGACTGCTGTCGCACCGGCTCGCGTCGGTGATCCGCGACTTCAACGAGCCGGTCATGATCTGGCAGGCCGC
GATTCTCGTCGGCGCGCTGGCGTTCGCCTGGGTCGCCGCGCGCTTCGTGCACGGCCGGATCGACGCACGCCGGCGGGCGG
CCGGCCGCCTGCCGGGCGCGGGTGCGCAAAGCCTGAAGCGCGCGCTCTTTCCGCTGTTCGGCGGGCTGTTCGTGCTGGCC
GCGCAGCTGGCGTTCGAGCCGTTCATGTCGACGTCGCTGCTGTCGCTCGCGCTGGTGCCGCTGTTCGGCATCGGGCTCAT
CTACGTGCTGTTTTTCTTCGCGCGGCGCGTGTTCGCGCGCGACGGCCACTCGCATGCGTGGCTCTCGATCGTCGAGAAGC
TCGTGTCGGTGATCGTGTGGGCCGCGATGGTGCTGACCGTGCTCGGCATCCAGCGCGACGTGCTCGACTGGCTCGACAGC
GTGCAGTTCCGCGTCGCGAACGCGCACCTGACGCTGCTGTCGCTGATCTCCGGCGCGCTGTGGGTCTGCGTGACGCTGAT
GGTCGCGATGTGGCTGGGCTCGGTGCTCGAGGATCGGCTCGCGCGCGCGAGCACGCTCGATGCGAACCTGCGCGTCGTGC
TCTCGCGCGTCGGCCGCGCGCTGCTCGTGTTCGCGGCGGTGCTGATCGGGCTGTCGCTGGTCGGCATCGACGTCACGGTG
CTCGGCGTGTTCGGCGGCGCGGTGGGCGTCGGCCTCGGCTTCGGGCTGCAGAAGATCGCGAGCAACTACGTGTCGGGCTT
CATCATCCTGCTGGACCGCTCGCTGCGGCTCGGCGACGCGATCAGCGTCGGCGGGCTGCAGGGCGTCGTCACGCAGATCC
GCACGCGCTACACGGTCGTACGCGGCCTCGACGGCAACGAGACGCTGATTCCGAACGAGAAGCTGATCACCGACGTCGTG
CAGAACCAGTCGTCGTACCTGACGCGCGGCTATGCGAAGCTCGCGGTGCAGGTCGCGTACACGAATGACGTCGAGCAGGC
ACTGGCGTTGCTTGCCGAGGCGGCGCGCGGCGTGCCGCGCGTGCTCGACGAGCCGGCGCCCACGCCGTATCTCGTCGGCT
TCGGCGCGGACGGGATCGACCTGGAGCTCGGCTTCTGGATCGCGGACGCGGCGACGGGCACCGGCAGCGTGCGCTCGAAC
GTGAACCGGAACATCTGGCGGCTATTTCATGAGCACGGGATCTCGATTCCGTACCCGCAGCGCGAGGTGCGCGTGATCGG
GCTGCCCGACGCGCTCGGCGGCACGGTGCGGGCGGCCAACGAGCCTCCCGCGGACGGCCGTGCGGCCGACGCGGCGCCCG
CGTCGAGCGCGCAGCAGCCGGCCGCGTGA

Upstream 100 bases:

>100_bases
CGATTACCTCTCGCTGTGGCCGCACCGCCACGCGACCGACGGCTTCTTCGCGGCCGTGCTCGAGCGGCGCGCGGCGCAGT
AACGGAGCAGGGCACGGACG

Downstream 100 bases:

>100_bases
CGCGCAGCGGCCGAAACGGGAGCGCAGCGGGCGCGGCGTCCGTCCGTGGACCGTTCCCGTTGCTTCTCGACAAGAAAATA
TTCATTTTTTACAAAGACTT

Product: MscS mechanosensitive ion channel

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MENRLLSHRLASVIRDFNEPVMIWQAAILVGALAFAWVAARFVHGRIDARRRAAGRLPGAGAQSLKRALFPLFGGLFVLA
AQLAFEPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHSHAWLSIVEKLVSVIVWAAMVLTVLGIQRDVLDWLDS
VQFRVANAHLTLLSLISGALWVCVTLMVAMWLGSVLEDRLARASTLDANLRVVLSRVGRALLVFAAVLIGLSLVGIDVTV
LGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKLAVQVAYTNDVEQALALLAEAARGVPRVLDEPAPTPYLVGFGADGIDLELGFWIADAATGTGSVRSN
VNRNIWRLFHEHGISIPYPQREVRVIGLPDALGGTVRAANEPPADGRAADAAPASSAQQPAA

Sequences:

>Translated_462_residues
MENRLLSHRLASVIRDFNEPVMIWQAAILVGALAFAWVAARFVHGRIDARRRAAGRLPGAGAQSLKRALFPLFGGLFVLA
AQLAFEPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHSHAWLSIVEKLVSVIVWAAMVLTVLGIQRDVLDWLDS
VQFRVANAHLTLLSLISGALWVCVTLMVAMWLGSVLEDRLARASTLDANLRVVLSRVGRALLVFAAVLIGLSLVGIDVTV
LGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKLAVQVAYTNDVEQALALLAEAARGVPRVLDEPAPTPYLVGFGADGIDLELGFWIADAATGTGSVRSN
VNRNIWRLFHEHGISIPYPQREVRVIGLPDALGGTVRAANEPPADGRAADAAPASSAQQPAA
>Mature_462_residues
MENRLLSHRLASVIRDFNEPVMIWQAAILVGALAFAWVAARFVHGRIDARRRAAGRLPGAGAQSLKRALFPLFGGLFVLA
AQLAFEPFMSTSLLSLALVPLFGIGLIYVLFFFARRVFARDGHSHAWLSIVEKLVSVIVWAAMVLTVLGIQRDVLDWLDS
VQFRVANAHLTLLSLISGALWVCVTLMVAMWLGSVLEDRLARASTLDANLRVVLSRVGRALLVFAAVLIGLSLVGIDVTV
LGVFGGAVGVGLGFGLQKIASNYVSGFIILLDRSLRLGDAISVGGLQGVVTQIRTRYTVVRGLDGNETLIPNEKLITDVV
QNQSSYLTRGYAKLAVQVAYTNDVEQALALLAEAARGVPRVLDEPAPTPYLVGFGADGIDLELGFWIADAATGTGSVRSN
VNRNIWRLFHEHGISIPYPQREVRVIGLPDALGGTVRAANEPPADGRAADAAPASSAQQPAA

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=258, Percent_Identity=34.4961240310078, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI2367355, Length=298, Percent_Identity=30.2013422818792, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1789291, Length=266, Percent_Identity=27.4436090225564, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49527; Mature: 49527

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]