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The map label for this gene is pbuE [H]

Identifier: 161523889

GI number: 161523889

Start: 778389

End: 779588

Strand: Direct

Name: pbuE [H]

Synonym: Bmul_0709

Alternate gene names: 161523889

Gene position: 778389-779588 (Clockwise)

Preceding gene: 161523888

Following gene: 161523894

Centisome position: 22.57

GC content: 72.5

Gene sequence:

>1200_bases
ATGAGCGAATGCGTATCGATGCCCGCAACGGCGGCCGGCGACCGCGTGCGTCTGCCGGTCGCGAATCTGCTCGCGCTTGC
GACGGCCGGCTTCATCACGGTGCTGACCGAAGCGCTGCCGGCCGGCCTGTTGCCGCTGATGAGCGCGGACCTGCGCGTGA
CCGAGGCGCTGATCGGCCAGCTCGTGACGGTCTATGCGCTCGGCTCGATCGTCGCCGCGATTCCATTGGTTGCCGCGACG
CGCGGGATCGCGCGCCGCCGTTTGTTGATCGCCGCGCTGGCCGGTTTCGTCGTGTCGAACGCGCTGACGGCCGTGTCGCC
GTACTACGTGCTCACGCTCGCCGCGCGCTTCGTCGCCGGCATGGCGGCCGGGCTGCTGTGGGCGCTGCTCGCGGGCTACG
CGAGCCGGATGGTCGACGAACACCTGCGTGGCCGCGCGATCGCGGTCGCAATGCTCGGCGTGCCGGTCGCGATGTCGATC
GGCATTCCGGCCGGCACCGCGCTCGGTGCGGCAGTCGGCTGGCGTGTCGCGTTCGCGGGCATCACGCTCGCGGCGCTCGC
GCTGATCGGATGGGTGCGGCTGCGTCTGCCCGACTACCCGGGGCAGCCGGCCGGTTCGCGCGAGCCCGTGCTCGCAGTGC
TGAAGATGCCGGGCGTGCGTGCGGTGCTGACGGTGATGTTCGCGTACGTGCTCGCGCACAACATGCTCTACACGTACATC
GCGCCGTTTCTCGCGGGTGAACGGATGGGCACGCAGGTCGATGCGGTGCTGTTCGTGTTCGGTGTCGCGTCGCTGGGCGG
GCTCGGGCTGACGGGCGCGTGGATCGGCGCCGCGCAGCGGCGGCTCACGTTCGCGAGCATCGCGTTGTTCGCGCTCGCGG
CGCTGATGCTCGGCGCCGGCTCCGGGATGGTGGTCGTCTATGCGGGCGTGGCGCTGTGGGGCATCGCGTTCGGCGGCGCG
CCGACGCTGTTCCAGACGGCCGCCGCGCAGTCGGCGGGCGACGCGGCCGACGTCGCGCAGTCGATGCTCGTCACGGTGTG
GAATCTCGCGATCGCCGGCGGCGGCATCGCGGGCGGCATGCTGCTTGACGCGGCGGGCGCCGGCCCGATTCCGTGGGTGC
TCGTCGCGCTGCTGAGCGCGGCCGCGCTCGGCGCATGGCGTGCGCGCGGGCATGCGTTTGCGGCGGCGCAGCGCGCGTGA

Upstream 100 bases:

>100_bases
GACTCACGGTCGTCGCGCTGACGAACACGCTGTACGAGGGCGCGCACGGCCGATTCGTCACCGACGTGCGCGATGCGGTC
TACGGCGCGGGGAGGGCGGC

Downstream 100 bases:

>100_bases
GCGTCGCGCCGTGGCGTCTGGCGCGCTGCTTCGGTAGCGCGCCGTCTCGGCATGGAGCCGACTACGCGTCGAGCGCCGCG
CGCGCGCAGGTCTCGTCCGT

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 398

Protein sequence:

>399_residues
MSECVSMPATAAGDRVRLPVANLLALATAGFITVLTEALPAGLLPLMSADLRVTEALIGQLVTVYALGSIVAAIPLVAAT
RGIARRRLLIAALAGFVVSNALTAVSPYYVLTLAARFVAGMAAGLLWALLAGYASRMVDEHLRGRAIAVAMLGVPVAMSI
GIPAGTALGAAVGWRVAFAGITLAALALIGWVRLRLPDYPGQPAGSREPVLAVLKMPGVRAVLTVMFAYVLAHNMLYTYI
APFLAGERMGTQVDAVLFVFGVASLGGLGLTGAWIGAAQRRLTFASIALFALAALMLGAGSGMVVVYAGVALWGIAFGGA
PTLFQTAAAQSAGDAADVAQSMLVTVWNLAIAGGGIAGGMLLDAAGAGPIPWVLVALLSAAALGAWRARGHAFAAAQRA

Sequences:

>Translated_399_residues
MSECVSMPATAAGDRVRLPVANLLALATAGFITVLTEALPAGLLPLMSADLRVTEALIGQLVTVYALGSIVAAIPLVAAT
RGIARRRLLIAALAGFVVSNALTAVSPYYVLTLAARFVAGMAAGLLWALLAGYASRMVDEHLRGRAIAVAMLGVPVAMSI
GIPAGTALGAAVGWRVAFAGITLAALALIGWVRLRLPDYPGQPAGSREPVLAVLKMPGVRAVLTVMFAYVLAHNMLYTYI
APFLAGERMGTQVDAVLFVFGVASLGGLGLTGAWIGAAQRRLTFASIALFALAALMLGAGSGMVVVYAGVALWGIAFGGA
PTLFQTAAAQSAGDAADVAQSMLVTVWNLAIAGGGIAGGMLLDAAGAGPIPWVLVALLSAAALGAWRARGHAFAAAQRA
>Mature_398_residues
SECVSMPATAAGDRVRLPVANLLALATAGFITVLTEALPAGLLPLMSADLRVTEALIGQLVTVYALGSIVAAIPLVAATR
GIARRRLLIAALAGFVVSNALTAVSPYYVLTLAARFVAGMAAGLLWALLAGYASRMVDEHLRGRAIAVAMLGVPVAMSIG
IPAGTALGAAVGWRVAFAGITLAALALIGWVRLRLPDYPGQPAGSREPVLAVLKMPGVRAVLTVMFAYVLAHNMLYTYIA
PFLAGERMGTQVDAVLFVFGVASLGGLGLTGAWIGAAQRRLTFASIALFALAALMLGAGSGMVVVYAGVALWGIAFGGAP
TLFQTAAAQSAGDAADVAQSMLVTVWNLAIAGGGIAGGMLLDAAGAGPIPWVLVALLSAAALGAWRARGHAFAAAQRA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=338, Percent_Identity=31.0650887573964, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1787808, Length=225, Percent_Identity=28.4444444444444, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787947, Length=261, Percent_Identity=34.0996168582375, Blast_Score=92, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 40395; Mature: 40263

Theoretical pI: Translated: 10.51; Mature: 10.51

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSECVSMPATAAGDRVRLPVANLLALATAGFITVLTEALPAGLLPLMSADLRVTEALIGQ
CCCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVTVYALGSIVAAIPLVAATRGIARRRLLIAALAGFVVSNALTAVSPYYVLTLAARFVAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAAGLLWALLAGYASRMVDEHLRGRAIAVAMLGVPVAMSIGIPAGTALGAAVGWRVAFAG
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHH
ITLAALALIGWVRLRLPDYPGQPAGSREPVLAVLKMPGVRAVLTVMFAYVLAHNMLYTYI
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
APFLAGERMGTQVDAVLFVFGVASLGGLGLTGAWIGAAQRRLTFASIALFALAALMLGAG
HHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SGMVVVYAGVALWGIAFGGAPTLFQTAAAQSAGDAADVAQSMLVTVWNLAIAGGGIAGGM
CCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
LLDAAGAGPIPWVLVALLSAAALGAWRARGHAFAAAQRA
HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
SECVSMPATAAGDRVRLPVANLLALATAGFITVLTEALPAGLLPLMSADLRVTEALIGQ
CCHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]