Click here to switch to the map view.

The map label for this gene is estB [H]

Identifier: 161523888

GI number: 161523888

Start: 777202

End: 778392

Strand: Direct

Name: estB [H]

Synonym: Bmul_0708

Alternate gene names: 161523888

Gene position: 777202-778392 (Clockwise)

Preceding gene: 161523886

Following gene: 161523889

Centisome position: 22.54

GC content: 73.47

Gene sequence:

>1191_bases
ATGTCATCCCTGCCTGTGATTGCCCGCGACAGCGAGGCCGACGTCGCGCTGGACGTGCGGCTTGACGGCGCGCTCGAGCG
CGCGCTCGCGGACGAACGGATCGTCGGCGCGGTCGTGATGGTCGCGCGCGACGGCGAGTTGCGCTACACGCGCGCGGCGG
GCCTTGCCGACCGCGAGGCCCGCACGCCGATGCGCGAGGACACGCTGTTCCGGCTCGCATCGGTCACGAAGCCGATCGTG
ACGGCCGCCGCGATGCGGCTCGTCGCGGCCGGCCGCATCGCGCTCGACGAACCGGTCGCGCGCTGGCTGCCCGAGTTCCG
GCCGACGCTGCGCGACGGCACGCCGGCCCGCATCACGTTGCGGCATCTGCTGTCGCATACGGCGGGCCTCGGCTATCGCT
TTCTCGAACCCGACGACGACGGTCCGTATGCGCGAGCCGGCGTATCCGACGGCATGGATCGCGCGCCCGTGTCGCTCGCG
GAGAACGTGCGGCGTATCGCGAGCGTGCCGCTGCAATTCGCGCCCGGTACGTCGTGGTGCTATTCGCTCGCGACGGACGT
GGTCGGTGCGCTGATCGAGGCCGTCCACGAAAGGCCGCTCGCCGAGGCGGTCGCCGAACTCGTCACGACACCGCTCGGCA
TGACCGCGACCGCGTTCGTCGCGCCCGATGCCGCGCGCGTCGCGACGCCGTACGTGAGTACGGCAGGCGCGCCGCGCCGG
ATGGCGGCCGTCGAGGTCGTACCGCCGCGCGACGGCACGGTCGGCATCCGCTTCGAACCGGCGCGTGCGTTCGATCGCGA
CGCATGGCCGTCGGGCGGCGCCGGCATGATCGGCTGCGCGGCGGACTGCGTCGCACTGCTCGACGCGCTGCGCACCGGCC
GCGACGGCTGGCTCGCGCCCAAGTGGGTCGACCAGATGGCGCGCATCCAGCCGGGCGCGCACGACGTGCCCGACGCGCCG
GGCTTCGGCTTCGGGCTCGGCTTTTCGGTGCTGCGCGATCCCGTCGCCGCGCAGTCGCCGGAGTCGGTCGGCACGTGGCG
CTGGGGCGGCGCGTACGGGCATGCGTGGTTCGTCGATCGCGCGGCCGGACTCACGGTCGTCGCGCTGACGAACACGCTGT
ACGAGGGCGCGCACGGCCGATTCGTCACCGACGTGCGCGATGCGGTCTACGGCGCGGGGAGGGCGGCATGA

Upstream 100 bases:

>100_bases
GCGGACGTCGATGTCCGGATTGTCCGGAACTCTAGCCCAGTTTTTCTTCGTTTGCCGGCTCCCTACGATGAGCGTTCGTT
CACTCATCGAGGTCATTGCC

Downstream 100 bases:

>100_bases
GCGAATGCGTATCGATGCCCGCAACGGCGGCCGGCGACCGCGTGCGTCTGCCGGTCGCGAATCTGCTCGCGCTTGCGACG
GCCGGCTTCATCACGGTGCT

Product: beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MSSLPVIARDSEADVALDVRLDGALERALADERIVGAVVMVARDGELRYTRAAGLADREARTPMREDTLFRLASVTKPIV
TAAAMRLVAAGRIALDEPVARWLPEFRPTLRDGTPARITLRHLLSHTAGLGYRFLEPDDDGPYARAGVSDGMDRAPVSLA
ENVRRIASVPLQFAPGTSWCYSLATDVVGALIEAVHERPLAEAVAELVTTPLGMTATAFVAPDAARVATPYVSTAGAPRR
MAAVEVVPPRDGTVGIRFEPARAFDRDAWPSGGAGMIGCAADCVALLDALRTGRDGWLAPKWVDQMARIQPGAHDVPDAP
GFGFGLGFSVLRDPVAAQSPESVGTWRWGGAYGHAWFVDRAAGLTVVALTNTLYEGAHGRFVTDVRDAVYGAGRAA

Sequences:

>Translated_396_residues
MSSLPVIARDSEADVALDVRLDGALERALADERIVGAVVMVARDGELRYTRAAGLADREARTPMREDTLFRLASVTKPIV
TAAAMRLVAAGRIALDEPVARWLPEFRPTLRDGTPARITLRHLLSHTAGLGYRFLEPDDDGPYARAGVSDGMDRAPVSLA
ENVRRIASVPLQFAPGTSWCYSLATDVVGALIEAVHERPLAEAVAELVTTPLGMTATAFVAPDAARVATPYVSTAGAPRR
MAAVEVVPPRDGTVGIRFEPARAFDRDAWPSGGAGMIGCAADCVALLDALRTGRDGWLAPKWVDQMARIQPGAHDVPDAP
GFGFGLGFSVLRDPVAAQSPESVGTWRWGGAYGHAWFVDRAAGLTVVALTNTLYEGAHGRFVTDVRDAVYGAGRAA
>Mature_395_residues
SSLPVIARDSEADVALDVRLDGALERALADERIVGAVVMVARDGELRYTRAAGLADREARTPMREDTLFRLASVTKPIVT
AAAMRLVAAGRIALDEPVARWLPEFRPTLRDGTPARITLRHLLSHTAGLGYRFLEPDDDGPYARAGVSDGMDRAPVSLAE
NVRRIASVPLQFAPGTSWCYSLATDVVGALIEAVHERPLAEAVAELVTTPLGMTATAFVAPDAARVATPYVSTAGAPRRM
AAVEVVPPRDGTVGIRFEPARAFDRDAWPSGGAGMIGCAADCVALLDALRTGRDGWLAPKWVDQMARIQPGAHDVPDAPG
FGFGLGFSVLRDPVAAQSPESVGTWRWGGAYGHAWFVDRAAGLTVVALTNTLYEGAHGRFVTDVRDAVYGAGRAA

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI26051231, Length=103, Percent_Identity=36.8932038834951, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI26051233, Length=103, Percent_Identity=36.8932038834951, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI87082101, Length=398, Percent_Identity=24.8743718592965, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17550934, Length=236, Percent_Identity=28.8135593220339, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI115533026, Length=224, Percent_Identity=25.8928571428571, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 42058; Mature: 41927

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPVIARDSEADVALDVRLDGALERALADERIVGAVVMVARDGELRYTRAAGLADREA
CCCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHEEEEEEEEECCCEEEEHHCCCCCHHH
RTPMREDTLFRLASVTKPIVTAAAMRLVAAGRIALDEPVARWLPEFRPTLRDGTPARITL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCHHHCCCCCHHHHH
RHLLSHTAGLGYRFLEPDDDGPYARAGVSDGMDRAPVSLAENVRRIASVPLQFAPGTSWC
HHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHH
YSLATDVVGALIEAVHERPLAEAVAELVTTPLGMTATAFVAPDAARVATPYVSTAGAPRR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCHHCCCCCCC
MAAVEVVPPRDGTVGIRFEPARAFDRDAWPSGGAGMIGCAADCVALLDALRTGRDGWLAP
EEEEEEECCCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
KWVDQMARIQPGAHDVPDAPGFGFGLGFSVLRDPVAAQSPESVGTWRWGGAYGHAWFVDR
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCEECCCCCCCHHEECC
AAGLTVVALTNTLYEGAHGRFVTDVRDAVYGAGRAA
CCCEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSLPVIARDSEADVALDVRLDGALERALADERIVGAVVMVARDGELRYTRAAGLADREA
CCCCEEEECCCCCEEEEEEECHHHHHHHHHHHHEEEEEEEEECCCEEEEHHCCCCCHHH
RTPMREDTLFRLASVTKPIVTAAAMRLVAAGRIALDEPVARWLPEFRPTLRDGTPARITL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHCHHHCCCCCHHHHH
RHLLSHTAGLGYRFLEPDDDGPYARAGVSDGMDRAPVSLAENVRRIASVPLQFAPGTSWC
HHHHHHHHCCCEEECCCCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCEECCCHHHH
YSLATDVVGALIEAVHERPLAEAVAELVTTPLGMTATAFVAPDAARVATPYVSTAGAPRR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCHHCCCCCCC
MAAVEVVPPRDGTVGIRFEPARAFDRDAWPSGGAGMIGCAADCVALLDALRTGRDGWLAP
EEEEEEECCCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCH
KWVDQMARIQPGAHDVPDAPGFGFGLGFSVLRDPVAAQSPESVGTWRWGGAYGHAWFVDR
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCHHCCCCCCCCCEECCCCCCCHHEECC
AAGLTVVALTNTLYEGAHGRFVTDVRDAVYGAGRAA
CCCEEEEEEHHHHHHCCCCCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]