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The map label for this gene is pykA [H]

Identifier: 161523851

GI number: 161523851

Start: 733813

End: 735249

Strand: Direct

Name: pykA [H]

Synonym: Bmul_0671

Alternate gene names: 161523851

Gene position: 733813-735249 (Clockwise)

Preceding gene: 161523850

Following gene: 161523852

Centisome position: 21.28

GC content: 67.08

Gene sequence:

>1437_bases
ATGCAGCGCGCCACCAAGATAGTCGCCACGATCGGCCCGGCTTCCAGTTCGCCGGAGATTCTGCTGCAGATGATGCAGGC
GGGCCTCGACGTCGTGCGGCTCAATTTTTCGCACGGCACGGCCGATGATCACCGCCAGCGCGCCGAAATGGTGCGCGAGG
CCGCCCGCAAGGTCGGCCGCGAGATCGCGATCATGGCCGATCTCCAGGGGCCGAAGATCCGCGTCGGCAAGTTCGAGAAC
GGCAAGACGACGCTGACGCCGGGCCAGCCCTTCATCCTCGACGCGGCCTGCGAACTGGGCAACGACGACCGCGTCGGCCT
CGACTACAAGGAACTGCCGCGCGACCTGAAGCCCGGCGATCTGCTGCTGCTGAACGACGGGCTGATCGTGCTGACCGTCG
AGCGCGTGCTCGGCGACGAGATCCACACGATCGTCAAGGTGGGCGGCGAGCTGTCGAACAACAAGGGGATCAACCGCCAG
GGCGGCGGGCTGTCGGCACCGGCGCTGACCGCGAAGGACATGGAGGACATCCGCACCGCGATGTCGCTCGGCGCGGATCT
CGTCGCGGTGTCGTTCCCGAAGAACGCGACCGACATGGAAATGGCGCGCCAGCTCGCGAACATCGCGGGTGCGCCGTACG
GCATCAAGCCGAAGATGATCGCGAAGATCGAGCGCGCGGAAGCGATTCCTGCGCTGCAGAGCATCCTCGACGCATCGGAC
GGCATCATGGTCGCGCGCGGCGACCTCGCGGTCGAAGTGGGCAACGCGGCCGTGCCCGCGCTGCAGAAGCGCATGATCCG
GATGGCGCGCGAGTCGAACAAGCTCGTGATCACCGCGACGCAGATGATGGAATCGATGATCCACGCGCCGGTGCCGACGC
GCGCGGAAGTGTCCGACGTCGCGAACGCGGTGCTCGACGGCACCGACGCGGTGATGCTGTCGGCCGAGACGGCCGCCGGC
AAGTATCCGGTGCAGACGATCGAGGCGATGGCGGCCGTCTGCGTCGAAGCGGAAAAGTCCGAGCAGATCGAGCTCGACAA
GGATTTCCTCGACCGCACGTTTACGCGCATCGACCAGTCGATCGCGATGGGCGCGCTGTTTACGGCCTACCACCTCGGCG
CGAAGGCGATCGTCGCGCTGACCGAATCGGGCGCGACCGCGTTGTGGATGTCGCGTCACTACACGCACGTGCCGATCTTC
GCGCTGACGCCGCGCGTCGGCAGCGAGCGCGCGATGGCGCTGTTCCGCAACGTGACGCCGCTGCACGTCGACTTCAACAG
CGACCGCGACAGCGCGCTGCAGGCGGCGCTCGAGACGATCGTCAAGCGCGGCTACGTCGCGCACGGCGACATGGTCGTGC
TGACCGTCGGCGAGCCGATGGGGCAGGCGGGCGGAACCAACACGCTGAAGATCGTGCGCGTCGGCGAACACTATTGA

Upstream 100 bases:

>100_bases
CACCGGCACGCCCCCCGGAGCGGCCGCCGCTGGGCCTGCCGCATCCGGCTCTCGCAGCGTTTCACCCAGCGCATCCACCT
TGATCCAGATGAGGAGTTTC

Downstream 100 bases:

>100_bases
GCGGTCGCGCAAGACGCGCCGGCCGGGCGGCCGGCGGCCCGATTCGATCGGGCACCGTTTTTGCTCGGCAAAAGCCGCGC
GGGGCGAGGCCCTGCGCGGC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLTPGQPFILDAACELGNDDRVGLDYKELPRDLKPGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVQTIEAMAAVCVEAEKSEQIELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQAALETIVKRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Sequences:

>Translated_478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLTPGQPFILDAACELGNDDRVGLDYKELPRDLKPGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVQTIEAMAAVCVEAEKSEQIELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQAALETIVKRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
>Mature_478_residues
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGREIAIMADLQGPKIRVGKFEN
GKTTLTPGQPFILDAACELGNDDRVGLDYKELPRDLKPGDLLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQ
GGGLSAPALTAKDMEDIRTAMSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDVANAVLDGTDAVMLSAETAAG
KYPVQTIEAMAAVCVEAEKSEQIELDKDFLDRTFTRIDQSIAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIF
ALTPRVGSERAMALFRNVTPLHVDFNSDRDSALQAALETIVKRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=37.0967741935484, Blast_Score=278, Evalue=1e-74,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=37.0967741935484, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286422, Length=493, Percent_Identity=36.3083164300203, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286420, Length=493, Percent_Identity=36.3083164300203, Blast_Score=277, Evalue=1e-74,
Organism=Homo sapiens, GI33286418, Length=493, Percent_Identity=35.9026369168357, Blast_Score=273, Evalue=2e-73,
Organism=Homo sapiens, GI310128732, Length=291, Percent_Identity=36.0824742268041, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128730, Length=291, Percent_Identity=36.0824742268041, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI310128736, Length=239, Percent_Identity=33.4728033472803, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI310128734, Length=239, Percent_Identity=33.4728033472803, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI310128738, Length=218, Percent_Identity=31.1926605504587, Blast_Score=105, Evalue=9e-23,
Organism=Escherichia coli, GI1788160, Length=478, Percent_Identity=52.3012552301255, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1787965, Length=473, Percent_Identity=38.6892177589852, Blast_Score=300, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.6803278688525, Blast_Score=285, Evalue=5e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=38.1930184804928, Blast_Score=261, Evalue=7e-70,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=37.5770020533881, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=38.1930184804928, Blast_Score=260, Evalue=1e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=37.5770020533881, Blast_Score=260, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=34.0862422997947, Blast_Score=258, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6324923, Length=492, Percent_Identity=35.9756097560976, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24648964, Length=497, Percent_Identity=38.2293762575453, Blast_Score=283, Evalue=3e-76,
Organism=Drosophila melanogaster, GI28571814, Length=497, Percent_Identity=38.2293762575453, Blast_Score=282, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24648966, Length=465, Percent_Identity=37.4193548387097, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI24581235, Length=489, Percent_Identity=31.4928425357873, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI24646914, Length=272, Percent_Identity=37.1323529411765, Blast_Score=149, Evalue=5e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51311; Mature: 51311

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
EIAIMADLQGPKIRVGKFENGKTTLTPGQPFILDAACELGNDDRVGLDYKELPRDLKPGD
EEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEHHHHCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETAAGKYPVQTIEAMAAVCVEAEKSEQIELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIFALTPRVGSERAMALFRNVTP
HHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSALQAALETIVKRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC
>Mature Secondary Structure
MQRATKIVATIGPASSSPEILLQMMQAGLDVVRLNFSHGTADDHRQRAEMVREAARKVGR
CCCHHEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHCC
EIAIMADLQGPKIRVGKFENGKTTLTPGQPFILDAACELGNDDRVGLDYKELPRDLKPGD
EEEEEEECCCCEEEEEEECCCCEEECCCCCEEEEHHHHCCCCCCCCCCHHHCCCCCCCCC
LLLLNDGLIVLTVERVLGDEIHTIVKVGGELSNNKGINRQGGGLSAPALTAKDMEDIRTA
EEEEECCEEEEEEHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
MSLGADLVAVSFPKNATDMEMARQLANIAGAPYGIKPKMIAKIERAEAIPALQSILDASD
HHCCCCEEEEECCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
GIMVARGDLAVEVGNAAVPALQKRMIRMARESNKLVITATQMMESMIHAPVPTRAEVSDV
CEEEEECCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCHHHHHH
ANAVLDGTDAVMLSAETAAGKYPVQTIEAMAAVCVEAEKSEQIELDKDFLDRTFTRIDQS
HHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCEECCHHHHHHHHHHHHHH
IAMGALFTAYHLGAKAIVALTESGATALWMSRHYTHVPIFALTPRVGSERAMALFRNVTP
HHHHHHHHHHHHCCEEEEEEECCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHCCCE
LHVDFNSDRDSALQAALETIVKRGYVAHGDMVVLTVGEPMGQAGGTNTLKIVRVGEHY
EEEECCCCHHHHHHHHHHHHHHCCCEECCCEEEEECCCCCCCCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]