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The map label for this gene is pgk [H]

Identifier: 161523850

GI number: 161523850

Start: 732332

End: 733528

Strand: Direct

Name: pgk [H]

Synonym: Bmul_0670

Alternate gene names: 161523850

Gene position: 732332-733528 (Clockwise)

Preceding gene: 161523849

Following gene: 161523851

Centisome position: 21.24

GC content: 67.92

Gene sequence:

>1197_bases
ATGAGCCAAGTCAAGCGTCTTACCGACCTGATCGCCGCCGGCCAGCTCGCCGGCAAGCGTGTGTTCATCCGCGCCGACCT
GAACGTCCCGCAGGACGATCACGGCAACATCACCGAAGACACGCGCGTGCGCGCCTCGGTGCCGGCCATCAAGGCGGCGC
TCGAAGCGGGCGCGGCGGTGATGGTCACGTCGCACCTCGGCCGTCCGACCGAAGGCGAGTTCAAGCCGGAAGACTCGCTC
GCGCCGGTCGCGAAGCGCCTGTCCGAGCTGCTCGGCCGCGACGTGCCGCTCGTCGCGAACTGGGTCGAGAACGGCGTGAA
CGTCGCGCCGGGCGAGGTCGTGCTGCTCGAGAACTGCCGCGTCAACAAGGGCGAGAAGAAGAATTCGGACGAGCTCGCGC
AAAAAATGGCGAAGCTCTGCGACGTGTACGTGAACGACGCGTTCGGCACCGCGCACCGCGCGGAAGCCACGACGCACGGG
ATCGCGAAATACGCGCCGGTCGCGTGCGCGGGCCCGCTGCTTGCTGCGGAACTCGACGCGCTCGGCAAGGCGCTCGGCAA
TCCGGCGCGGCCGCTCGTCGCGATCGTCGCGGGCTCGAAGGTGTCGACCAAGCTGACGATCCTGAAGTCGCTGGCCGAAA
AGGTCGACCAGCTGATCGTCGGCGGCGGGATCGCGAACACGTTCATGCTCGCGGCCGGCCTGTCGATCGGCAAGTCGCTC
GCCGAACCGGATCTCGTCGACGAGGCGAAGGCGATCATCGACGAAGCGCGCAAGCGCGGCGCGTCGGTGCCGATCCCGAC
CGACGTCGTGACCGCGAAGGAATTCTCGCCGACGGCCGCAGCGACCGTGAAGCCCGTCGCCGACATCGAAGCCGACGACA
TGATCCTCGACATCGGCCCGGACACGGCAAAGGCGCTCGCGAGCCAGCTCGAGAAGGCCGGCACGATCGTCTGGAACGGG
CCGGTCGGCGTGTTCGAGTTCGACCAGTTCGGCAACGGCACGAAGACGCTCGCCGAAGCGATCGCGAAGTCGTCCGCGTT
CTCGATCGCAGGCGGCGGCGACACGCTCGCGGCCATCGCGAAGTACGGCATCCACGACCAGGTCAGCTACATCTCGACGG
GCGGCGGCGCCTTCCTCGAGTTCCTCGAAGGGAAGAAGCTGCCGGCAGTGGAAGTGCTCGAAACGCGGGCGGCCTGA

Upstream 100 bases:

>100_bases
TGCGGAGCCGCCAGCCACGGCCGCATCCGGCGCCCTTTCGCGGCAGCCCGCGCACGTCCGGCGTACCCCTTTCCCCATCC
ACTACTTCAATCAGTCCAAC

Downstream 100 bases:

>100_bases
GCGTGGCGGCGCGCGCAGGGGCGACGAAGGCCGCGCGCTCCGCGAAAGCGGAGCCGGCGGCCGGCGCGGGCAAGCGCAAA
CGCGCGCCCGCGCGGGCGAA

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIKAALEAGAAVMVTSHLGRPTEGEFKPEDSL
APVAKRLSELLGRDVPLVANWVENGVNVAPGEVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLSIGKSL
AEPDLVDEAKAIIDEARKRGASVPIPTDVVTAKEFSPTAAATVKPVADIEADDMILDIGPDTAKALASQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA

Sequences:

>Translated_398_residues
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIKAALEAGAAVMVTSHLGRPTEGEFKPEDSL
APVAKRLSELLGRDVPLVANWVENGVNVAPGEVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHG
IAKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLSIGKSL
AEPDLVDEAKAIIDEARKRGASVPIPTDVVTAKEFSPTAAATVKPVADIEADDMILDIGPDTAKALASQLEKAGTIVWNG
PVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
>Mature_397_residues
SQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIKAALEAGAAVMVTSHLGRPTEGEFKPEDSLA
PVAKRLSELLGRDVPLVANWVENGVNVAPGEVVLLENCRVNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGI
AKYAPVACAGPLLAAELDALGKALGNPARPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLSIGKSLA
EPDLVDEAKAIIDEARKRGASVPIPTDVVTAKEFSPTAAATVKPVADIEADDMILDIGPDTAKALASQLEKAGTIVWNGP
VGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIAKYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI31543397, Length=421, Percent_Identity=42.2802850356295, Blast_Score=285, Evalue=4e-77,
Organism=Homo sapiens, GI4505763, Length=402, Percent_Identity=41.044776119403, Blast_Score=274, Evalue=9e-74,
Organism=Escherichia coli, GI1789294, Length=397, Percent_Identity=63.9798488664987, Blast_Score=477, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI17508823, Length=419, Percent_Identity=42.9594272076372, Blast_Score=298, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6319857, Length=408, Percent_Identity=43.3823529411765, Blast_Score=284, Evalue=2e-77,
Organism=Drosophila melanogaster, GI17136394, Length=406, Percent_Identity=40.8866995073892, Blast_Score=259, Evalue=3e-69,
Organism=Drosophila melanogaster, GI24581203, Length=418, Percent_Identity=37.799043062201, Blast_Score=241, Evalue=4e-64,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 41331; Mature: 41199

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIKAALEAGAAV
CCHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFKPEDSLAPVAKRLSELLGRDVPLVANWVENGVNVAPGEVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPARPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLSIGKSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEPDLVDEAKAIIDEARKRGASVPIPTDVVTAKEFSPTAAATVKPVADIEADDMILDIGP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCCCEEEECCC
DTAKALASQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
CHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHH
KYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
HHCCCHHHHHEECCCHHHHHHHCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SQVKRLTDLIAAGQLAGKRVFIRADLNVPQDDHGNITEDTRVRASVPAIKAALEAGAAV
CHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEE
MVTSHLGRPTEGEFKPEDSLAPVAKRLSELLGRDVPLVANWVENGVNVAPGEVVLLENCR
EEEHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCEEEEECCC
VNKGEKKNSDELAQKMAKLCDVYVNDAFGTAHRAEATTHGIAKYAPVACAGPLLAAELDA
CCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHCCHHHHHHHHH
LGKALGNPARPLVAIVAGSKVSTKLTILKSLAEKVDQLIVGGGIANTFMLAAGLSIGKSL
HHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AEPDLVDEAKAIIDEARKRGASVPIPTDVVTAKEFSPTAAATVKPVADIEADDMILDIGP
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHCCHHHCCCCCCEEEECCC
DTAKALASQLEKAGTIVWNGPVGVFEFDQFGNGTKTLAEAIAKSSAFSIAGGGDTLAAIA
CHHHHHHHHHHHCCEEEECCCCCEEECCCCCCCHHHHHHHHHHCCCEEECCCCHHHHHHH
KYGIHDQVSYISTGGGAFLEFLEGKKLPAVEVLETRAA
HHCCCHHHHHEECCCHHHHHHHCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]