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The map label for this gene is ubiE [H]

Identifier: 161523747

GI number: 161523747

Start: 618951

End: 619682

Strand: Direct

Name: ubiE [H]

Synonym: Bmul_0567

Alternate gene names: 161523747

Gene position: 618951-619682 (Clockwise)

Preceding gene: 161523746

Following gene: 161523748

Centisome position: 17.95

GC content: 59.02

Gene sequence:

>732_bases
ATGAGCAAAACCCACTTCGGCTTCGAAACCGTCGAGGAAACCGATAAAGCGAAGAAAGTGGCGGGCGTGTTCCATTCGGT
CGCGAACAACTACGACCTGATGAACGATCTGATGTCGGCCGGCATGCACCGCGCGTGGAAGGCGTTCACGATCGCGCAGG
CGAACGTGCGGCCCGGCTACAAGGTGCTCGACATCGCGGCCGGCACCGGCGACCTGACGAAGGCGTTCGCGAAGGCGGCC
GGCCCGACGGGCGAAGTCTGGCACACGGACATCAACGAATCGATGCTGCGCGTCGGCCGCGACCGGCTGCTCGACAAGGG
CATCGTGACGCCGTCGCTGCTTTGCGACGCGGAGAAAATTCCCTTTCCGGACAACTACTTCGACGTCGTCACGGTCGCGT
TCGGGCTGCGCAACATGACGCACAAGGACGTCGCGCTGGCCGAGATGCGCCGCGTCGCGAAGCCGGGCGGCCGCGTGATG
GTGCTGGAATTCTCGAAAGTGTGGGAGCCGCTGAAAAAAGCGTACGATTTGTATTCTTTCAAAGTATTACCGTGGCTTGG
CGACAAGTTCGCGAAAGATGCCGACAGTTACAGGTATCTTGCCGAATCTATCCGGATGCACCCGGATCAGGACACGTTGA
AGACGATGATGGAACAAGCGGGCCTCGATGCCGTCAAATATTACAATTTGTCAGGTGGCGTGGTAGCTTTACACCTCGGA
ACCAAGTACTAA

Upstream 100 bases:

>100_bases
GATGCGCCGATGCCGGCGGCGAGCGCGCCGCACGGCCACTGCCACTGAAATCGACGGCGCCCGTTCGGCGCCGTCTTGCC
ATTTACTGAGGATCAACGCG

Downstream 100 bases:

>100_bases
GGGGTTCTTTCCATACATTTGTCTTGCATCTGGAGAAGCTGATGTCCGAATCGCGATCGTTGTTCAACCGTAGCAAGCCG
TCGAAGCCGTGGGCTCGACG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSKTHFGFETVEETDKAKKVAGVFHSVANNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGIVTPSLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVAKPGGRVM
VLEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHLG
TKY

Sequences:

>Translated_243_residues
MSKTHFGFETVEETDKAKKVAGVFHSVANNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTKAFAKAA
GPTGEVWHTDINESMLRVGRDRLLDKGIVTPSLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVAKPGGRVM
VLEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHLG
TKY
>Mature_242_residues
SKTHFGFETVEETDKAKKVAGVFHSVANNYDLMNDLMSAGMHRAWKAFTIAQANVRPGYKVLDIAAGTGDLTKAFAKAAG
PTGEVWHTDINESMLRVGRDRLLDKGIVTPSLLCDAEKIPFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVAKPGGRVMV
LEFSKVWEPLKKAYDLYSFKVLPWLGDKFAKDADSYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHLGT
KY

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=277, Percent_Identity=43.3212996389892, Blast_Score=219, Evalue=2e-57,
Organism=Escherichia coli, GI48994983, Length=243, Percent_Identity=61.7283950617284, Blast_Score=305, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17557039, Length=244, Percent_Identity=44.672131147541, Blast_Score=213, Evalue=9e-56,
Organism=Saccharomyces cerevisiae, GI6323526, Length=257, Percent_Identity=42.0233463035019, Blast_Score=167, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24641702, Length=255, Percent_Identity=45.8823529411765, Blast_Score=221, Evalue=3e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27120; Mature: 26988

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS01183 UBIE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTHFGFETVEETDKAKKVAGVFHSVANNYDLMNDLMSAGMHRAWKAFTIAQANVRPGY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGIVTPSLLCDAEKI
EEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVAKPGGRVMVLEFSKVWEPLKKAYDLYSF
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDADSYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHLG
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEC
TKY
CCC
>Mature Secondary Structure 
SKTHFGFETVEETDKAKKVAGVFHSVANNYDLMNDLMSAGMHRAWKAFTIAQANVRPGY
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCC
KVLDIAAGTGDLTKAFAKAAGPTGEVWHTDINESMLRVGRDRLLDKGIVTPSLLCDAEKI
EEEEEECCCCHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHCCCCCHHHHCCCCCC
PFPDNYFDVVTVAFGLRNMTHKDVALAEMRRVAKPGGRVMVLEFSKVWEPLKKAYDLYSF
CCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHHE
KVLPWLGDKFAKDADSYRYLAESIRMHPDQDTLKTMMEQAGLDAVKYYNLSGGVVALHLG
EECHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEECCCCEEEEEEC
TKY
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA