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The map label for this gene is nagE [H]

Identifier: 161523662

GI number: 161523662

Start: 527085

End: 528875

Strand: Direct

Name: nagE [H]

Synonym: Bmul_0482

Alternate gene names: 161523662

Gene position: 527085-528875 (Clockwise)

Preceding gene: 161523661

Following gene: 161523667

Centisome position: 15.28

GC content: 68.62

Gene sequence:

>1791_bases
ATGGACGGGAATCCGTTTCTGAAGATACAGAGCCTGGGACGGGCGCTGATGCTGCCGATCGCGGTGTTGCCGGTCGCCGG
CATCCTGCTGCGGCTCGGGCAGCCGGACGTGTTCAACATCAAGATGATCGCCGATGCCGGCGGCGCGATCTTCGACAACC
TGCCGCTCTTGTTCGCGATCGGCGTGGCGGTCGGCTTCGCGAAGGACAACAACGGCGTCGCGGCGCTCGCGGGCGCGATC
GGCTATCTGATCGAAACCGCGATCATGAAGGACATCGACCCGAAGCTGAACATGGGCGTGCTGTCCGGCATCATCGCGGG
TGTCGTGGCCGGCCTGCTGTACAACCGCTACAAGGACATCAAGCTGCCCGACTACCTCGCGTTCTTCGGCGGCAAGCGGT
TCGTGCCGATCATCACGGGGCTCGTATGCGTGGTGCTCGGCATCGTGTTCGGTTACGTGTGGCAGCCGATCCAACATGCG
ATCGACGCGGCCGGCCAGTGGCTGACGACGGCGGGCGCGATCGGAGCGTTCGTGTTCGGCTTCCTGAACCGGCTGCTGCT
CGTCACGGGGCTGCACCACATCATCAACTCGCTCGCGTGGTTCGTGTTCGGCAACTTCACGCCGCCCGCCGGCGGCGAGA
TCGTGCACGGCGACCTGCACCGCTTCTTCGCGGGCGACCCGACCGCGGGCACCTTCATGGCCGGTTTCTTCCCGATCATG
ATGTTCGGCCTGCCGGCCGCATGTCTCGCGATGCTGCACGAAGCGCCAAAGGAGCGCCGCGCGATGGTCGGCGGCCTGCT
GTTCTCGATGGCGTTGACCTCGTTCCTCACGGGCGTGACCGAGCCGATCGAATTCAGCTTCATGTTCCTCGCGCCGGTGC
TGTACGTGATCCACGCGGTGCTGACGGGGCTGTCGCTCGCGATCTGCCAGATCCTCGGCGTGAAGCTCGGCTTCACGTTC
TCGGCCGGCGCGATCGACTACGTGCTGAACTACGGGCTGTCGACGAAGGGCTGGATCGCGATTCCGCTCGGCATCGCGTA
CGGCATCGCGTATTACGCACTGTTCCGCTTCTTCATCCGCACCTTCAACATGGCGACGCCGGGCCGCGAGCCCGCATCGG
CCGATGCGGCGAGCGAATCGTATGCATCCGGCGGCTTCGTCGCGCCGGCGGCCGGCGCTGCGGGCACTGCGACGGCCGCG
GCCGCCGCGCCGCGTGCGCAGCGCTATATCGCGGCGCTCGGCGGTGCCGGCAACCTGAGCGTCGTCGATGCATGCACGAC
GCGTCTGCGTCTGACCGTCGTCGATCCGGCCAAGGTATCGGAGCCGGAGCTCAAGTCGATCGGTGCGCGCGGCGTGCTCA
AGCGCGGCGGCAACAGCGTGCAGGTGATCATCGGGCCCGAGGCCGACATCATTGCGGACGAGATGCGCGCGGCGATCGGC
GGCGGCGCAGCAGGCGCGGCCGTGAACGCGACGACGCCTGCGGGCGCCGTCGCGGAGCCTGCCGCAGGCGCGGCAGCCGG
CCCGCTCGATCCGGAGCCCGCGCGCTGGCTCGCGGTGTTCGGCGGCGCGACGAACGTCGTGTCGCTCGACGCGATCGCGA
CCACGCGTTTGCGCGTCGTCGTGCGCGATCCGTCGGCGGTCGATCGCGAGCGCCTCGGCACGCTCGACGTCGCATGGGTG
TCGTCGGACACGCTGCACATCGTCTGCGGCAATGCGGCTGCGCGCTACGCGCAGCAGCTCGGTGCGCGTCTGCCGTCGGC
CGGCGACGGCGCGGCCGCGCAGCCGGCCTGA

Upstream 100 bases:

>100_bases
GAGATCTGGCCGCTCGACTGAGTCGTCGGCGGCGGGCTCGCGGCCCGTTTTACTACGAAAGCAATCAAGTCAACGACGAG
ACAATGGACTGGAGGACTGA

Downstream 100 bases:

>100_bases
CGCGACGACGCCGGCCCGCATCCGCATAGCGGATCGGCGGCCGGCCGGCAAAACGGAAACGGCTTGCGATGCGATCGCAA
GCCGTTTTCTTTTGGCGCGG

Product: PTS system N-acetylglucosamine-specific transporter subunit IIBC

Products: NA

Alternate protein names: EIICBA-Nag; EII-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component; N-acetylglucosamine-specific phosphotransferase enzyme IIA component; PTS system N-acetylglucosamine-specific EIIA component [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHA
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGIAYYALFRFFIRTFNMATPGREPASADAASESYASGGFVAPAAGAAGTATAA
AAAPRAQRYIAALGGAGNLSVVDACTTRLRLTVVDPAKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIG
GGAAGAAVNATTPAGAVAEPAAGAAAGPLDPEPARWLAVFGGATNVVSLDAIATTRLRVVVRDPSAVDRERLGTLDVAWV
SSDTLHIVCGNAAARYAQQLGARLPSAGDGAAAQPA

Sequences:

>Translated_596_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHA
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGIAYYALFRFFIRTFNMATPGREPASADAASESYASGGFVAPAAGAAGTATAA
AAAPRAQRYIAALGGAGNLSVVDACTTRLRLTVVDPAKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIG
GGAAGAAVNATTPAGAVAEPAAGAAAGPLDPEPARWLAVFGGATNVVSLDAIATTRLRVVVRDPSAVDRERLGTLDVAWV
SSDTLHIVCGNAAARYAQQLGARLPSAGDGAAAQPA
>Mature_596_residues
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAIGVAVGFAKDNNGVAALAGAI
GYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDIKLPDYLAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHA
IDAAGQWLTTAGAIGAFVFGFLNRLLLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
MFGLPAACLAMLHEAPKERRAMVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAVLTGLSLAICQILGVKLGFTF
SAGAIDYVLNYGLSTKGWIAIPLGIAYGIAYYALFRFFIRTFNMATPGREPASADAASESYASGGFVAPAAGAAGTATAA
AAAPRAQRYIAALGGAGNLSVVDACTTRLRLTVVDPAKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIG
GGAAGAAVNATTPAGAVAEPAAGAAAGPLDPEPARWLAVFGGATNVVSLDAIATTRLRVVVRDPSAVDRERLGTLDVAWV
SSDTLHIVCGNAAARYAQQLGARLPSAGDGAAAQPA

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=476, Percent_Identity=55.672268907563, Blast_Score=497, Evalue=1e-142,
Organism=Escherichia coli, GI1787343, Length=500, Percent_Identity=44.6, Blast_Score=393, Evalue=1e-110,
Organism=Escherichia coli, GI1787908, Length=522, Percent_Identity=36.9731800766284, Blast_Score=279, Evalue=5e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011055
- InterPro:   IPR018113
- InterPro:   IPR004719
- InterPro:   IPR001127
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00358 PTS_EIIA_1; PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 61615; Mature: 61615

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KLPDYLAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHAIDAAGQWLTTAGAIGAFVFG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLNRLLLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHH
MFGLPAACLAMLHEAPKERRAMVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQILGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGIAYGIAYYALFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
TFNMATPGREPASADAASESYASGGFVAPAAGAAGTATAAAAAPRAQRYIAALGGAGNLS
HHCCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCH
VVDACTTRLRLTVVDPAKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIG
HHHHHCCEEEEEEECCCCCCCCHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHC
GGAAGAAVNATTPAGAVAEPAAGAAAGPLDPEPARWLAVFGGATNVVSLDAIATTRLRVV
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEHHEEEEEEEEE
VRDPSAVDRERLGTLDVAWVSSDTLHIVCGNAAARYAQQLGARLPSAGDGAAAQPA
EECCCHHHHHHCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MDGNPFLKIQSLGRALMLPIAVLPVAGILLRLGQPDVFNIKMIADAGGAIFDNLPLLFAI
CCCCCEEEHHHHCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHCCHHHHHH
GVAVGFAKDNNGVAALAGAIGYLIETAIMKDIDPKLNMGVLSGIIAGVVAGLLYNRYKDI
HHHHEEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
KLPDYLAFFGGKRFVPIITGLVCVVLGIVFGYVWQPIQHAIDAAGQWLTTAGAIGAFVFG
CCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
FLNRLLLVTGLHHIINSLAWFVFGNFTPPAGGEIVHGDLHRFFAGDPTAGTFMAGFFPIM
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECHHHHHHCCCCCCHHHHHHHHHHH
MFGLPAACLAMLHEAPKERRAMVGGLLFSMALTSFLTGVTEPIEFSFMFLAPVLYVIHAV
HHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LTGLSLAICQILGVKLGFTFSAGAIDYVLNYGLSTKGWIAIPLGIAYGIAYYALFRFFIR
HHHHHHHHHHHHHHHHCCEECCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHHH
TFNMATPGREPASADAASESYASGGFVAPAAGAAGTATAAAAAPRAQRYIAALGGAGNLS
HHCCCCCCCCCCCCCCCHHHHCCCCEECCCCCCCCCHHHHHHCHHHHHHHHHHCCCCCCH
VVDACTTRLRLTVVDPAKVSEPELKSIGARGVLKRGGNSVQVIIGPEADIIADEMRAAIG
HHHHHCCEEEEEEECCCCCCCCHHHHCCCCHHHHCCCCEEEEEECCCCHHHHHHHHHHHC
GGAAGAAVNATTPAGAVAEPAAGAAAGPLDPEPARWLAVFGGATNVVSLDAIATTRLRVV
CCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEEEHHEEEEEEEEE
VRDPSAVDRERLGTLDVAWVSSDTLHIVCGNAAARYAQQLGARLPSAGDGAAAQPA
EECCCHHHHHHCCCEEEEEECCCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1745234 [H]