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The map label for this gene is gshB [H]

Identifier: 161523620

GI number: 161523620

Start: 485113

End: 486069

Strand: Direct

Name: gshB [H]

Synonym: Bmul_0440

Alternate gene names: 161523620

Gene position: 485113-486069 (Clockwise)

Preceding gene: 161523619

Following gene: 161523621

Centisome position: 14.07

GC content: 68.76

Gene sequence:

>957_bases
ATGGACATTCTCTTTATCGCCGACCCGCTCGAGCGCTTCAAGATCTACAAGGATTCGACCTACGCGATGATGGCCGAGGC
CGCGCGGCGCGGCCATGCGATCTACGCGTGCGAGCCGCGCGAGCTCGCCTGGACGGGCTCGGCGGTCGAGGCCGACGTGC
GGCGCATCACGTTCGTCGGCGAACTGGACGATCTGCATCGCGAGACCTGGTACGAGGCGGGGCCCGTCGACGCGCGTCGC
CTCGAGTCGTTCGGCGCGGTGCTGATGCGCAAGGATCCGCCGTTCGACATGGAATACGTGACGTCGACGTGGCTGCTCGA
ACTGGCCGAGCGGGCGGGCGCGCGCGTGTTCAACCGCGCGCAGTCGATCCGCGACCATTCGGAAAAGCTCGCGATCGGCG
AGTTCCCGCAGTTCGTCGCGCCGACGCTCGTCACGCGCGACGCGAAACGGCTGCGCGCATTCCATGCGGAGCACGGCGAC
GTGATCCTGAAGCCGCTCGACGGCATGGGCGGCATGGGCGTGTTCCGCGTGAAGCCGGACGGCATGAACCTCGGTTCGAT
CATCGAGATGCTGAGCCACGACGGCACGCGCTCGGTGATGGCGCAGAAGTTCATTCCCGAGATCAAGGCCGGCGACAAGC
GCATTCTGCTGATCGGCGGCGAGCCGGTGCCGTATTCGCTGGCGCGGATTCCGCAGGGCAGCGAGGTGCGCGGCAATCTC
GCGGCCGGCGGCCTTGGCGTCGCGCAGCCGCTCACGGCGCGCGACCGCGAGATCGCCGAGACGCTGGGCCCGGTGCTCGC
CGCGCGCGGGCTGCTGCTCGTCGGCCTCGACGCGATCGGCGACTGGCTCACGGAGGTGAACGTGACGAGCCCGACCTGCT
TCCGCGAAATCATGGAGCAGACGGGCTTCGACGTCGCCGCGATGTTCATCGACGCGCTGGAGCGGGCGGCCGCGTAG

Upstream 100 bases:

>100_bases
CGTCGCGCGCCTGTCGCTGATCGCATCGTCGATCGAACTCGAAAAGACCGATCCCGAAGCCATTCAGGTGTAACCGGGCT
TCGCTACGTACAGGACCCGC

Downstream 100 bases:

>100_bases
GCACCCGCCGCGTCACACCGGCGGCGGCCGCGCAGGCCCGCCGCCGCCGGATCGGCATGACCGGCCTGCTACAATGCCCG
CTCGTCCGGCGCCGCGATCG

Product: glutathione synthetase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MDILFIADPLERFKIYKDSTYAMMAEAARRGHAIYACEPRELAWTGSAVEADVRRITFVGELDDLHRETWYEAGPVDARR
LESFGAVLMRKDPPFDMEYVTSTWLLELAERAGARVFNRAQSIRDHSEKLAIGEFPQFVAPTLVTRDAKRLRAFHAEHGD
VILKPLDGMGGMGVFRVKPDGMNLGSIIEMLSHDGTRSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAETLGPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMEQTGFDVAAMFIDALERAAA

Sequences:

>Translated_318_residues
MDILFIADPLERFKIYKDSTYAMMAEAARRGHAIYACEPRELAWTGSAVEADVRRITFVGELDDLHRETWYEAGPVDARR
LESFGAVLMRKDPPFDMEYVTSTWLLELAERAGARVFNRAQSIRDHSEKLAIGEFPQFVAPTLVTRDAKRLRAFHAEHGD
VILKPLDGMGGMGVFRVKPDGMNLGSIIEMLSHDGTRSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAETLGPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMEQTGFDVAAMFIDALERAAA
>Mature_318_residues
MDILFIADPLERFKIYKDSTYAMMAEAARRGHAIYACEPRELAWTGSAVEADVRRITFVGELDDLHRETWYEAGPVDARR
LESFGAVLMRKDPPFDMEYVTSTWLLELAERAGARVFNRAQSIRDHSEKLAIGEFPQFVAPTLVTRDAKRLRAFHAEHGD
VILKPLDGMGGMGVFRVKPDGMNLGSIIEMLSHDGTRSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
AAGGLGVAQPLTARDREIAETLGPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMEQTGFDVAAMFIDALERAAA

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=310, Percent_Identity=47.4193548387097, Blast_Score=289, Evalue=1e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 35000; Mature: 35000

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDILFIADPLERFKIYKDSTYAMMAEAARRGHAIYACEPRELAWTGSAVEADVRRITFVG
CCEEEEECHHHHHEECCCCHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHEEEEC
ELDDLHRETWYEAGPVDARRLESFGAVLMRKDPPFDMEYVTSTWLLELAERAGARVFNRA
CHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSIRDHSEKLAIGEFPQFVAPTLVTRDAKRLRAFHAEHGDVILKPLDGMGGMGVFRVKPD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCC
GMNLGSIIEMLSHDGTRSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCC
AAGGLGVAQPLTARDREIAETLGPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMEQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAAA
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDILFIADPLERFKIYKDSTYAMMAEAARRGHAIYACEPRELAWTGSAVEADVRRITFVG
CCEEEEECHHHHHEECCCCHHHHHHHHHHCCCEEEEECCCCEEECCCHHHHHHHHEEEEC
ELDDLHRETWYEAGPVDARRLESFGAVLMRKDPPFDMEYVTSTWLLELAERAGARVFNRA
CHHHHHHHHHCCCCCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QSIRDHSEKLAIGEFPQFVAPTLVTRDAKRLRAFHAEHGDVILKPLDGMGGMGVFRVKPD
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCEEEECCC
GMNLGSIIEMLSHDGTRSVMAQKFIPEIKAGDKRILLIGGEPVPYSLARIPQGSEVRGNL
CCCHHHHHHHHHCCCHHHHHHHHHCHHHCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCC
AAGGLGVAQPLTARDREIAETLGPVLAARGLLLVGLDAIGDWLTEVNVTSPTCFREIMEQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCHHEEEHHHHHHHHHHCCCCCHHHHHHHHHH
TGFDVAAMFIDALERAAA
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]