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The map label for this gene is lipB [H]

Identifier: 161523596

GI number: 161523596

Start: 456087

End: 456833

Strand: Direct

Name: lipB [H]

Synonym: Bmul_0416

Alternate gene names: 161523596

Gene position: 456087-456833 (Clockwise)

Preceding gene: 161523595

Following gene: 161523597

Centisome position: 13.23

GC content: 67.47

Gene sequence:

>747_bases
ATGTCCGTCTCGCCGGTTGCCATTGTGTCCACGCCCGTCGCCGCTCCCGAATCGTCCGCCGCCGTCGCGGTCCCGCCGGT
CGTCGTGCGCTGGCGCGGCGTCGAGCCGTACGAGACGAGCTTCGACGCGATGCGCGCATTCACCGACGCGCGCACGGCCG
ACACCGTCGACGAGATCTGGATCGTCGAACATCCGCCCGTCTACACGCTCGGCCAGGCCGGCGATCCCGCCCATCTGCTG
GTGGGAGACAGCGGCATTCCGCTCGTGAAGGTCGACCGCGGCGGGCAAATCACCTATCACGGCCCCGGCCAGATCGTCGC
CTACCTGCTGCTCGACTTGCGCCGCCGCAAGCTGACGGTGCGCACCTTGGTGACGAAGATCGAGGAGGCCGTGATCGAAA
CCCTCGCGGCGTATAATCTCGCTTCGGTCCGCAAGGCGGGCGCGCCGGGGATCTACGTGGCATCCGGCGTGCACGAGGGC
GCGAAGATCGCCGCCCTCGGCCTGAAGATCCGCAACGGCTGCAGCTATCACGGGCTGAGCCTGAACGTGAAGATGGATCT
GCGCCCGTTTCTCGCGATCAATCCGTGCGGCTATGCCGGACTGGAAACCGTCGACATGGCGAGCCTCGAGGTTGCCGCCG
ACTGGAACGACGTGGCCCGCACGCTGGTGCGGCGTCTGATCGCCAACCTCGACGGCGAATCCGCGGCCGCCGACACGCCG
CACGCACTGGAACATTCGAATGACTGA

Upstream 100 bases:

>100_bases
GGCTGGCGAACCGTCTGACGCGCTTTCGTCGGACGGATCGCCGCGATCCGTCCCTTTCCGCGGCCGGCGAGACCCATGGG
TTTTCGGTAAACTACCGCCC

Downstream 100 bases:

>100_bases
CGTTACCGCTTCTCCCGCACCGGCCGATTCGGCCGCGACCCCTGCGTACAACCCGACCGCCAAGCAGAAGGCGCAGGCGA
AGACGGCGCGCATTCCGATC

Product: lipoate-protein ligase B

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MSVSPVAIVSTPVAAPESSAAVAVPPVVVRWRGVEPYETSFDAMRAFTDARTADTVDEIWIVEHPPVYTLGQAGDPAHLL
VGDSGIPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLTVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEG
AKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVARTLVRRLIANLDGESAAADTP
HALEHSND

Sequences:

>Translated_248_residues
MSVSPVAIVSTPVAAPESSAAVAVPPVVVRWRGVEPYETSFDAMRAFTDARTADTVDEIWIVEHPPVYTLGQAGDPAHLL
VGDSGIPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLTVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEG
AKIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVARTLVRRLIANLDGESAAADTP
HALEHSND
>Mature_247_residues
SVSPVAIVSTPVAAPESSAAVAVPPVVVRWRGVEPYETSFDAMRAFTDARTADTVDEIWIVEHPPVYTLGQAGDPAHLLV
GDSGIPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLTVRTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGA
KIAALGLKIRNGCSYHGLSLNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVARTLVRRLIANLDGESAAADTPH
ALEHSND

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI87081767, Length=206, Percent_Identity=51.9417475728155, Blast_Score=216, Evalue=9e-58,
Organism=Saccharomyces cerevisiae, GI6323268, Length=163, Percent_Identity=31.2883435582822, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21355483, Length=156, Percent_Identity=33.974358974359, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 26397; Mature: 26266

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: PS01313 LIPB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVSPVAIVSTPVAAPESSAAVAVPPVVVRWRGVEPYETSFDAMRAFTDARTADTVDEIW
CCCCCEEEEECCCCCCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHEE
IVEHPPVYTLGQAGDPAHLLVGDSGIPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLTV
EEECCCEEEECCCCCCCEEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHH
RTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYHGLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCEE
LNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVARTLVRRLIANLDGESAAADTP
EEEEECCCCEEEECCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCC
HALEHSND
CCCCCCCC
>Mature Secondary Structure 
SVSPVAIVSTPVAAPESSAAVAVPPVVVRWRGVEPYETSFDAMRAFTDARTADTVDEIW
CCCCEEEEECCCCCCCCCCEEECCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHEE
IVEHPPVYTLGQAGDPAHLLVGDSGIPLVKVDRGGQITYHGPGQIVAYLLLDLRRRKLTV
EEECCCEEEECCCCCCCEEEECCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHH
RTLVTKIEEAVIETLAAYNLASVRKAGAPGIYVASGVHEGAKIAALGLKIRNGCSYHGLS
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEEEEEEEEECCCCCCCEE
LNVKMDLRPFLAINPCGYAGLETVDMASLEVAADWNDVARTLVRRLIANLDGESAAADTP
EEEEECCCCEEEECCCCCCCCCEEECCEEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCC
HALEHSND
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA