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The map label for this gene is hisF [H]

Identifier: 161523514

GI number: 161523514

Start: 364948

End: 365721

Strand: Direct

Name: hisF [H]

Synonym: Bmul_0334

Alternate gene names: 161523514

Gene position: 364948-365721 (Clockwise)

Preceding gene: 161523513

Following gene: 161523515

Centisome position: 10.58

GC content: 69.38

Gene sequence:

>774_bases
ATGGCTCTAGCTAAACGCATCATCCCCTGCCTGGACGTGACTGCCGGGCGTGTCGTCAAGGGCGTCAACTTCGTCGAACT
GCGCGACGCGGGCGACCCCGTCGAGATCGCCCGCCGCTACGACGAGCAGGGCGCCGACGAACTGACGTTCCTCGACATCA
CCGCGACGTCCGACCAGCGCGACCTGATCCTGCCGATCATCGAGGCCGTCGCGTCGCAGGTCTTCATCCCGCTGACGGTC
GGCGGCGGCGTGCGCGCCGTCGAGGACGTGCGGCGCCTGCTCAACGCGGGCGCGGACAAGGTCAGCATGAATTCGTCGGC
GGTCGCGAATCCGCAGCTCGTGCGCGACGCGGCCGACAAGTACGGTTCGCAGTGCATCGTCGTCGCGATCGACGCGAAGC
GCGTGTCGGCCGACGGCGAAGCGCCGCGCTGGGAAGTGTTCACGCACGGCGGCCGCAAGGGCACGGGCCTCGACGCGATC
GAATGGGCGCGCAAGATGGCCGAGTTCGGCGCGGGCGAGATCCTGCTCACGAGCATGGACCGCGACGGCACGAAGTCGGG
CTTCGACCTCGCGCTCACGCGCGGCGTCTCCGACGCGGTGCCGGTGCCGGTGATCGCATCGGGCGGCGTCGGCTCGCTGC
AGGATCTGGCCGACGGCATCAAGGACGGCCGTGCGGATGCGGTGCTCGCCGCGAGCATCTTCCACTACGGCGAGCACACG
GTCGGCGAAGCGAAGCGCTTCATGGCCGAGCAGGGCATTCCGGTGAGGCTGTGA

Upstream 100 bases:

>100_bases
CTGCGGGCGGCCCGACCGGGCGTCTCGTGCGAGACGTCCGCAACCGGCCGCCCGACCGGCCGTAACCGGCCGTCCCCGTC
GCGGCATTTGGCGCAACATC

Downstream 100 bases:

>100_bases
TGAATACCGAAACGAAACCGCTGCCCGCGTGGCTCGACAAGGTCCGCTGGGACGACAACGGCCTCGTGCCGGTGATCGCA
CAGGAAGCGTCGACGAACGA

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKGTGLDAI
EWARKMAEFGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQDLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMAEQGIPVRL

Sequences:

>Translated_257_residues
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTV
GGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKGTGLDAI
EWARKMAEFGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQDLADGIKDGRADAVLAASIFHYGEHT
VGEAKRFMAEQGIPVRL
>Mature_256_residues
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQRDLILPIIEAVASQVFIPLTVG
GGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADKYGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKGTGLDAIE
WARKMAEFGAGEILLTSMDRDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQDLADGIKDGRADAVLAASIFHYGEHTV
GEAKRFMAEQGIPVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=31.404958677686, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=34.7133757961783, Blast_Score=169, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27214; Mature: 27083

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQR
CCHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKGTGLDAIEWARKMAEFGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQDLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMAEQGIPVRL
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
ALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDEQGADELTFLDITATSDQR
CHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEEEEECCCCC
DLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPQLVRDAADK
CHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHH
YGSQCIVVAIDAKRVSADGEAPRWEVFTHGGRKGTGLDAIEWARKMAEFGAGEILLTSMD
HCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEEEECC
RDGTKSGFDLALTRGVSDAVPVPVIASGGVGSLQDLADGIKDGRADAVLAASIFHYGEHT
CCCCCCCCCEEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCC
VGEAKRFMAEQGIPVRL
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA