Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 161523512

GI number: 161523512

Start: 363259

End: 363900

Strand: Direct

Name: hisH

Synonym: Bmul_0332

Alternate gene names: 161523512

Gene position: 363259-363900 (Clockwise)

Preceding gene: 161523511

Following gene: 161523513

Centisome position: 10.53

GC content: 66.51

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGGATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCAAGAAGGCCGAGCC
GGCCGCCGACGTGGCGATCGTCGATACGCCGGCCGCGATTCGCGCAGCCGACCGCGTCGTGCTGCCGGGCCAGGGCGCGA
TGCCGGACTGCATGCGCTGCCTCGGCGAATCGGGCCTGCAGGAGGCCGTCGTCGAAGCGTCGCGCACGAAGCCGCTGCTC
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAAGGGGCTGGGGCTGCTGCCCGGCAA
GGTCGTGCGCTTCGAACTCGACGGCCGGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGCG
TGCGCCAGTCGCGCGCGCACGCGCTGTGGGACGGCGTGCCCGACGATGCGTATTTCTATTTCGTGCACAGCTATTACGTG
ATGCCGGACGATCCGGCGCATACGGTCGGCGAAACGGCGTACGGCGCGCCGTTTACGTCCGCGGTCGCGCGCGACAACAT
CTTCGCGACCCAATTCCACCCGGAAAAAAGCGCGGAGGTCGGGTTGCGTCTGTATCGCAACTTCGTACACTGGAAACCGT
AA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGCCACGCGGCTGATGGGTCTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCAACGGACTGAGG
GCGCTATTGCCTGCACTGAG

Downstream 100 bases:

>100_bases
ACGTGGTGCGCGAACCACAGTCGACGGGCCGGAAACGCCCGTCGTACGGGGCTCGCGCGCGTAGGCGCCGAAAGAGTTGT
ACTAAACTAGCGAGACGGCG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSRAHALWDGVPDDAYFYFVHSYYV
MPDDPAHTVGETAYGAPFTSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWKP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSRAHALWDGVPDDAYFYFVHSYYV
MPDDPAHTVGETAYGAPFTSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWKP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDDGSRFKVPQMGWNRVRQSRAHALWDGVPDDAYFYFVHSYYV
MPDDPAHTVGETAYGAPFTSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWKP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.5384615384615, Blast_Score=123, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=32.1100917431193, Blast_Score=102, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_BURM1 (Q845U9)

Other databases:

- EMBL:   AB091436
- EMBL:   CP000868
- EMBL:   AP009385
- RefSeq:   YP_001578524.1
- RefSeq:   YP_001947343.1
- ProteinModelPortal:   Q845U9
- SMR:   Q845U9
- GeneID:   5765415
- GeneID:   6359502
- GenomeReviews:   AP009385_GR
- GenomeReviews:   CP000868_GR
- KEGG:   bmj:BMULJ_02922
- KEGG:   bmu:Bmul_0332
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13146
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23359; Mature: 23359

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 83-83 ACT_SITE 193-193 ACT_SITE 195-195

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSRAHALWDGVPDDAYFYFVHSYYVMPDDPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNIFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALKKAEPAADVAIVDTPAAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEEECCHHHHHCCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFELDGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCCEEEEEEECCEECCC
GSRFKVPQMGWNRVRQSRAHALWDGVPDDAYFYFVHSYYVMPDDPAHTVGETAYGAPFTS
CCEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCCCCHHCCCCCCCCCHHH
AVARDNIFATQFHPEKSAEVGLRLYRNFVHWKP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231