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The map label for this gene is thiE [H]

Identifier: 161523496

GI number: 161523496

Start: 349751

End: 350866

Strand: Direct

Name: thiE [H]

Synonym: Bmul_0316

Alternate gene names: 161523496

Gene position: 349751-350866 (Clockwise)

Preceding gene: 161523495

Following gene: 161523497

Centisome position: 10.14

GC content: 75.18

Gene sequence:

>1116_bases
ATGAGCGCGCGCTTTGCCGACGCCTTCTGGCCGCCCGCCGACGAGCTCGCCGAAGCGGCCGAACGCATCCGCGCGCGGCT
CGGCGACTGGCCGCGAGCCGCCGCGCCGTGGCGGCTCTGCATGACCGCGCCCGACGCGCCGGCCGACGGCGACGTGCTGA
TCGTGTCGCGCGGCGATCGCGCGGGGCAGGCGCGCGCATCGGCCGTGTCGCGGCCCGCATCGCCGAACGCCGTCGCGATC
GAATTCGACGAGCGCGGCGCGGCGCTTCACGCGGCCGACGCGCGCTACGCGCTCGACGCCGCGCATGCGCTCGCGGACGA
CTGGATCGCGGCGCTCGCCGCGTTTCTCGATTGCGGTTTCGCGCCGCTCGACGCGCTGGTGCTCGCGCTCGCGTGGCGCG
ACGGCGACGAGACGCGCGCCGACGACGCATGGCCCGTCGACGCCGCGCAGTTTCCGCGCGTGGCCGGGCTGCCGGCCGCC
GCCGAGCCGGCATTCGCGCCGTGCCCGGCGCGCCTCGGCCTCTATCCGGTCGTACCGGACGCCGAGTGGGTCGAACGCGT
GCTCGACTGCGGCGTGCAGACGATACAGCTGCGCGTGAAGGGCGCGGCGCCGGACGTGCTGCGCCGCGAAATCGCGCGCG
CGGTCGCGGCGGGCCGCCGCTATCCGGATGCGCGCGTGTTCATCAACGATCACTGGCAGATCGCGGCAGATGAGGGCGCG
TACGGCGTACATCTCGGCCAGGAAGATCTGGAAACGGCCGATCTCGCCGCGCTCGCACGCGCGGGGCTGCGGCTCGGGCT
GTCGAGCCACGGCTATTACGAGATGCTGCGCGCGCTGCACGAACGGCCGAGCTACCTCGCGCTCGGGCCCGTGTTCGCGA
CCGCGACCAAGGCCGTCGCCGCGCCGCCGCAGGGGCTCGCGCGGATCGCGCGCTACGCGCGCTTCGCCAGCCCGCGCGTG
CCGCTCGTCGCGATCGGCGGCGTCGGGCTCGACGCCTTGCCGGCCGTGCTCGCGACCGGCGTCGGCAGTGTCGCGGTGGT
CAGTGCGGTGACCGGCGCGGGCGATTACCGCGCGGCGATCGCGGCGCTGCAGCGGTGCTTTGCGTCCCCTATTTGA

Upstream 100 bases:

>100_bases
GCGCCGCCTATCTCGCCGGCCCGATGGACGCACGTGAAACCGCGCACGCGAGCACGCCGGTCGTCGGGATGCCGTTCTGG
CATCAGGACGGAGGCGGCGC

Downstream 100 bases:

>100_bases
CAATCGTTGACCGCGCGGTCGGGAAAGGCGTCACGACATCATTCCGAGGCAGGCCCTATAATTCGGCGTTCTGCGTAAAA
GGACTGTCCGCTCCGTGAGC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSARFADAFWPPADELAEAAERIRARLGDWPRAAAPWRLCMTAPDAPADGDVLIVSRGDRAGQARASAVSRPASPNAVAI
EFDERGAALHAADARYALDAAHALADDWIAALAAFLDCGFAPLDALVLALAWRDGDETRADDAWPVDAAQFPRVAGLPAA
AEPAFAPCPARLGLYPVVPDAEWVERVLDCGVQTIQLRVKGAAPDVLRREIARAVAAGRRYPDARVFINDHWQIAADEGA
YGVHLGQEDLETADLAALARAGLRLGLSSHGYYEMLRALHERPSYLALGPVFATATKAVAAPPQGLARIARYARFASPRV
PLVAIGGVGLDALPAVLATGVGSVAVVSAVTGAGDYRAAIAALQRCFASPI

Sequences:

>Translated_371_residues
MSARFADAFWPPADELAEAAERIRARLGDWPRAAAPWRLCMTAPDAPADGDVLIVSRGDRAGQARASAVSRPASPNAVAI
EFDERGAALHAADARYALDAAHALADDWIAALAAFLDCGFAPLDALVLALAWRDGDETRADDAWPVDAAQFPRVAGLPAA
AEPAFAPCPARLGLYPVVPDAEWVERVLDCGVQTIQLRVKGAAPDVLRREIARAVAAGRRYPDARVFINDHWQIAADEGA
YGVHLGQEDLETADLAALARAGLRLGLSSHGYYEMLRALHERPSYLALGPVFATATKAVAAPPQGLARIARYARFASPRV
PLVAIGGVGLDALPAVLATGVGSVAVVSAVTGAGDYRAAIAALQRCFASPI
>Mature_370_residues
SARFADAFWPPADELAEAAERIRARLGDWPRAAAPWRLCMTAPDAPADGDVLIVSRGDRAGQARASAVSRPASPNAVAIE
FDERGAALHAADARYALDAAHALADDWIAALAAFLDCGFAPLDALVLALAWRDGDETRADDAWPVDAAQFPRVAGLPAAA
EPAFAPCPARLGLYPVVPDAEWVERVLDCGVQTIQLRVKGAAPDVLRREIARAVAAGRRYPDARVFINDHWQIAADEGAY
GVHLGQEDLETADLAALARAGLRLGLSSHGYYEMLRALHERPSYLALGPVFATATKAVAAPPQGLARIARYARFASPRVP
LVAIGGVGLDALPAVLATGVGSVAVVSAVTGAGDYRAAIAALQRCFASPI

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=203, Percent_Identity=54.1871921182266, Blast_Score=202, Evalue=2e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 38971; Mature: 38840

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSARFADAFWPPADELAEAAERIRARLGDWPRAAAPWRLCMTAPDAPADGDVLIVSRGDR
CCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
AGQARASAVSRPASPNAVAIEFDERGAALHAADARYALDAAHALADDWIAALAAFLDCGF
CCHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
APLDALVLALAWRDGDETRADDAWPVDAAQFPRVAGLPAAAEPAFAPCPARLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCEECCCC
AEWVERVLDCGVQTIQLRVKGAAPDVLRREIARAVAAGRRYPDARVFINDHWQIAADEGA
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
YGVHLGQEDLETADLAALARAGLRLGLSSHGYYEMLRALHERPSYLALGPVFATATKAVA
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHC
APPQGLARIARYARFASPRVPLVAIGGVGLDALPAVLATGVGSVAVVSAVTGAGDYRAAI
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
AALQRCFASPI
HHHHHHHCCCC
>Mature Secondary Structure 
SARFADAFWPPADELAEAAERIRARLGDWPRAAAPWRLCMTAPDAPADGDVLIVSRGDR
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCEEEEECCCC
AGQARASAVSRPASPNAVAIEFDERGAALHAADARYALDAAHALADDWIAALAAFLDCGF
CCHHHHHHHCCCCCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
APLDALVLALAWRDGDETRADDAWPVDAAQFPRVAGLPAAAEPAFAPCPARLGLYPVVPD
CHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCEECCCC
AEWVERVLDCGVQTIQLRVKGAAPDVLRREIARAVAAGRRYPDARVFINDHWQIAADEGA
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCEEEEECCCC
YGVHLGQEDLETADLAALARAGLRLGLSSHGYYEMLRALHERPSYLALGPVFATATKAVA
CEEECCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEECHHHHHHHHHHC
APPQGLARIARYARFASPRVPLVAIGGVGLDALPAVLATGVGSVAVVSAVTGAGDYRAAI
CCHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHH
AALQRCFASPI
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA