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The map label for this gene is gudD [H]

Identifier: 161523381

GI number: 161523381

Start: 223429

End: 224826

Strand: Reverse

Name: gudD [H]

Synonym: Bmul_0201

Alternate gene names: 161523381

Gene position: 224826-223429 (Counterclockwise)

Preceding gene: 161523382

Following gene: 161523376

Centisome position: 6.52

GC content: 70.17

Gene sequence:

>1398_bases
ATGTCCGAAGCCCGTCATGCCGGCACGCCGCGCGTCACGCGGATGCAGGTGATTCCCGTCGCCGGCCGCGACAGCATGCT
GCTCAATCTCTGCGGCGCGCATGCACCGTATTTCACGCGCAACCTCGTGATACTCGACGACAGCAGCGGACGCACCGGCG
TCGGCGAAGTGCCGGGCGGTGAAGGTATCCGGCACGCGCTCGAGCGCATGACCGATCTCGTCGTCGGCCAGCCGATCGGC
CGCTACCAGGCGACGCTGAACGCGGTGCGCGCGGCGCTGTCCGGCGCGGCCGCGGGCGCGGGCCGCACGATCCGGCACGA
GGTCACGTCGGCCGGCGAAGCCGCGGTGCTGCGCCAGCCGCACGAGATCAACCTGCGGCTCGACAACGTGATCACCGCGA
TCGAGGCCGCGCTGCTCGACCTGCTCGGCCAGTATCTCGACGTGCCGGTTGCCGCGCTGCTCGGCGAAGGTCAACAGCGC
GACGCGGTGCCGATGCTCGCGTATCTGTTCTATATCGGCGACCGGCGCCGCACCGATCTGCCGTATCGCGACGAGGCGCA
CGCCAGCGATCCCTGGTTCCGGCTGCGCAACGACGAGGCGCTGACGCCGGCCGCGATCGCGCGGCAGGCGGAGGCCGCGG
TGGAACGCTACGGGTTCGCCGATTTCAAGCTGAAGGGCGGCGTGATGGACGGCGCCGACGAGATGGAAGCGATCGCGGCG
ATCAAGGCGCGCTTTCCGGACGCGCGCGTGACGCTCGATCCGAACGGCGCCTGGTCGCTCGACGAAGCGATCGCGCTGTG
CCGCGGGCAAGGCCACCTGCTCGCGTATGCGGAAGATCCGTGCGGGCCGGAAGCCGGCTATTCGGGGCGCGAGGTCATGG
CCGAATTCCGTCGCGCGACCGGCATTCCGACGGCGACCAACATGATCGCGACCGACTGGCGGCAGATGGATCATGCGGTG
CGGCTGCAGGCCGTCGACATCCCGCTCGCCGACCCGCACTTCTGGACGATGCAGGGCTCGGTCCGGCTCGCGCAGCTGTG
CCGCGACTGGGGGCTCACGTGGGGCTCGCACTCGAACAATCACTTCGACGTGTCGCTCTCGATGTTCACGCATGCGGCAG
CCGCGGCGCCGGGCACGATCACCGCGATCGACACGCACTGGATCTGGCAGGAAGGCGATGCGCGGCTCACGCGCGAACCG
CTCGAGATCGTCGGCGGCAAGGTCGCCGTGCCGGAGCGGCCGGGGCTCGGGATCGAGCTGGATCTCGCGCAGGTTGAGGC
CGCTCATGCGCTGTACCGGCAGGTGGGCGGCACCGCGCGCGACGATGCGGTCGCGATGCGGTATCTCGTGCCGGGGTGGA
CCTACGATCCGAAGCGGCCGAGTTTCGGGCGGCGCTAG

Upstream 100 bases:

>100_bases
CTGTTCGTCGTCGGCGAGATCCGCCGCGTCGAGCTTCGTTGAACCGACGCGGGCCGAGCCGGCGCGGCCGGCCGCCCGCC
TTCGAGAGGAACCCCGAACG

Downstream 100 bases:

>100_bases
GGCGGCGGCGCGCATAAACAAAAAGGGCTCGATCTTGCGATCGAGCCCTTCGAATTCGTTGGTGGAGCGGAGGAGGATCG
AACTCCCGACCTTCGCATTG

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSEARHAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGRTGVGEVPGGEGIRHALERMTDLVVGQPIG
RYQATLNAVRAALSGAAAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQYLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRRRTDLPYRDEAHASDPWFRLRNDEALTPAAIARQAEAAVERYGFADFKLKGGVMDGADEMEAIAA
IKARFPDARVTLDPNGAWSLDEAIALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLSMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LEIVGGKVAVPERPGLGIELDLAQVEAAHALYRQVGGTARDDAVAMRYLVPGWTYDPKRPSFGRR

Sequences:

>Translated_465_residues
MSEARHAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGRTGVGEVPGGEGIRHALERMTDLVVGQPIG
RYQATLNAVRAALSGAAAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQYLDVPVAALLGEGQQR
DAVPMLAYLFYIGDRRRTDLPYRDEAHASDPWFRLRNDEALTPAAIARQAEAAVERYGFADFKLKGGVMDGADEMEAIAA
IKARFPDARVTLDPNGAWSLDEAIALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAV
RLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLSMFTHAAAAAPGTITAIDTHWIWQEGDARLTREP
LEIVGGKVAVPERPGLGIELDLAQVEAAHALYRQVGGTARDDAVAMRYLVPGWTYDPKRPSFGRR
>Mature_464_residues
SEARHAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGRTGVGEVPGGEGIRHALERMTDLVVGQPIGR
YQATLNAVRAALSGAAAGAGRTIRHEVTSAGEAAVLRQPHEINLRLDNVITAIEAALLDLLGQYLDVPVAALLGEGQQRD
AVPMLAYLFYIGDRRRTDLPYRDEAHASDPWFRLRNDEALTPAAIARQAEAAVERYGFADFKLKGGVMDGADEMEAIAAI
KARFPDARVTLDPNGAWSLDEAIALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRATGIPTATNMIATDWRQMDHAVR
LQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNNHFDVSLSMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPL
EIVGGKVAVPERPGLGIELDLAQVEAAHALYRQVGGTARDDAVAMRYLVPGWTYDPKRPSFGRR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=457, Percent_Identity=63.2385120350109, Blast_Score=596, Evalue=1e-172,
Organism=Escherichia coli, GI1789151, Length=456, Percent_Identity=58.7719298245614, Blast_Score=540, Evalue=1e-154,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50577; Mature: 50446

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEARHAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGRTGVGEVPGG
CCCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQPIGRYQATLNAVRAALSGAAAGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQYLDVPVAALLGEGQQRDAVPMLAYLFYIGDRRRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAHASDPWFRLRNDEALTPAAIARQAEAAVERYGFADFKLKGGVMDGADEMEAIAA
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IKARFPDARVTLDPNGAWSLDEAIALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRAT
HHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLSMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLEIVGGKVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHCCCEEECCCCCCCCEEE
DLAQVEAAHALYRQVGGTARDDAVAMRYLVPGWTYDPKRPSFGRR
EHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEARHAGTPRVTRMQVIPVAGRDSMLLNLCGAHAPYFTRNLVILDDSSGRTGVGEVPGG
CCCCCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCHHHCEEEEEECCCCCCCCCCCCCC
EGIRHALERMTDLVVGQPIGRYQATLNAVRAALSGAAAGAGRTIRHEVTSAGEAAVLRQP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHCCC
HEINLRLDNVITAIEAALLDLLGQYLDVPVAALLGEGQQRDAVPMLAYLFYIGDRRRTDL
CEEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCC
PYRDEAHASDPWFRLRNDEALTPAAIARQAEAAVERYGFADFKLKGGVMDGADEMEAIAA
CCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHH
IKARFPDARVTLDPNGAWSLDEAIALCRGQGHLLAYAEDPCGPEAGYSGREVMAEFRRAT
HHHCCCCCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHC
GIPTATNMIATDWRQMDHAVRLQAVDIPLADPHFWTMQGSVRLAQLCRDWGLTWGSHSNN
CCCCHHHHHHHHHHHHHHHEEEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCC
HFDVSLSMFTHAAAAAPGTITAIDTHWIWQEGDARLTREPLEIVGGKVAVPERPGLGIEL
EEEEEHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCHHHHCCCEEECCCCCCCCEEE
DLAQVEAAHALYRQVGGTARDDAVAMRYLVPGWTYDPKRPSFGRR
EHHHHHHHHHHHHHHCCCCCCHHHHHEEECCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]