Click here to switch to the map view.

The map label for this gene is livK [C]

Identifier: 161523366

GI number: 161523366

Start: 209969

End: 211111

Strand: Direct

Name: livK [C]

Synonym: Bmul_0186

Alternate gene names: 161523366

Gene position: 209969-211111 (Clockwise)

Preceding gene: 161523365

Following gene: 161523368

Centisome position: 6.09

GC content: 63.87

Gene sequence:

>1143_bases
ATGAATATCAAGATGCAAAAGCTGTTGCCGATCACCGCGGCGGCGATGCTGTGCGCTGCAGCCGCAAGCAACGCGTCCGC
CGATCAAGTCGTCAAGATCGGCCACGTCGCGCCGTTGACGGGCGGCATTGCACACCTGGGCAAGGACAACGAAAACGGTG
CACGTCTCGCAGTCGAGGAGATCAACGCGAAGGGACTGACGATCGGCGGCCAGAAGATCACGCTGCAGCTCGACCCGCAG
GATGATGCGGCCGACCCGCGTCAGGCGACGCAGGTTGCGCAGAAGCTCGTCGACGACAAGGTCGTCGCGGTGGTCGGCCA
CCTGAACTCGGGCACGTCGATTCCGGCCTCGAAGATCTACAGCGATGCGGGCATCGTGCAGATCTCGCCGTCGGCGACGA
ACCCGGCCTACACGCAGCAGGGCTTCAAGACGACGTATCGCGTCGTCGCGACCGACGCGCAGCAGGGCCCGGCACTCGCG
AACTACGCGCATTCGAAGGGCATCAAGAGCGTCGCCGTGGTCGACGATTCGACCGCCTACGGTCAGGGCCTCGCGAACGA
ATTCGAGAAGAAGGCGAAGGCGCTTGGCCTGAAGGTGGTGTCGCACGACGCGACGAACGACAAGGCGGTCGACTTCCGCG
CGATTCTGACGAAGATCAAGGGTGAAAACCCCGACGCGATCATGTACGGCGGCATGGACGCCACCGGCGGCCCGTTCGCG
AAGCAGGCGAAGCAGCTCGGCCTGCGCGCGAAGATCTTCGCGGGCGACGGCGTGTGCACGGAAAAGCTGGCCGACCTCGC
CGGCGACGCGACCGACAACGTCGTGTGCTCGGAAGCCGGTGCATCGCTCGAGAAGATGCCGGGCGGCGCCGCGTTCAAGG
CGAAGTACGAGAAGCGTTTCGGCCAGCCGATCCAGATCTATGCGCCGTTCACGTATGACGCCGTGTACATCATCGTCGAC
GCGATGAAGCGCGCGAACTCGACCGATCCGGCGAAGATCGTCGCGGCGATGCCGGCGACGAACTACACGGGCGTGATCGG
CACGACGACCTTCGATTCGAAGGGCGACCTGCGGCACGGCGTGATCTCGCTCTACAACTACAAGAGCGGCAAGAAGACGC
TGCTCGACGAAGTGAAGATGTAA

Upstream 100 bases:

>100_bases
ATGAACGATGCAACCGGCGAATCGTCCAGTGATGCAAACACAGGGATGGCAGCGGGGCACCGGGCGATGGCGTTTATTGG
GACCGAAACTGGAGACTTAC

Downstream 100 bases:

>100_bases
CGCGACAAGCGGTCAACAGCAGGGGTGAAAACGCGCATGCGGCGACGCATGCGCGTTTTCTTTTGCGCGGGCGCGCCGCT
CAGATCTTCAGATGCGCGAG

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDPQ
DDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPATNYTGVIGTTTFDSKGDLRHGVISLYNYKSGKKTLLDEVKM

Sequences:

>Translated_380_residues
MNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDPQ
DDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPATNYTGVIGTTTFDSKGDLRHGVISLYNYKSGKKTLLDEVKM
>Mature_380_residues
MNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKITLQLDPQ
DDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYAHSKGIKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAIMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPATNYTGVIGTTTFDSKGDLRHGVISLYNYKSGKKTLLDEVKM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=357, Percent_Identity=33.6134453781513, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI48994941, Length=370, Percent_Identity=32.4324324324324, Blast_Score=179, Evalue=3e-46,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39809; Mature: 39809

Theoretical pI: Translated: 8.90; Mature: 8.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYAHSKGIKSVAVVDDSTAY
CCCCEEEECCCCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAIMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPATNYTGVIGTTTFDSKGDLRHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHC
VISLYNYKSGKKTLLDEVKM
EEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MNIKMQKLLPITAAAMLCAAAASNASADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHHHHHHHHHHHHHHCCCCCHHHEEEECCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKITLQLDPQDDAADPRQATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYAHSKGIKSVAVVDDSTAY
CCCCEEEECCCCCCCHHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAIMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPATNYTGVIGTTTFDSKGDLRHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCHHHC
VISLYNYKSGKKTLLDEVKM
EEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA