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The map label for this gene is nylA [H]

Identifier: 161523363

GI number: 161523363

Start: 205985

End: 207463

Strand: Reverse

Name: nylA [H]

Synonym: Bmul_0183

Alternate gene names: 161523363

Gene position: 207463-205985 (Counterclockwise)

Preceding gene: 161523364

Following gene: 161523339

Centisome position: 6.02

GC content: 72.48

Gene sequence:

>1479_bases
ATGCATGACGACTATCTCGCGCACGACGCGATCGGCCTCGCGACGCTCGTGCGCGAACGCCAGGCAAGCCCGCTCGAACT
GCTCGACGCGGCCATCGGCCAGGCCGACGCCGTCAACGGCGCGATCAACGCGATCGTGCTGCAGGATTACGATGCCGCGC
GCACGCGTGCGGCGGCCGCGTCCGCGGCAACCGCCGACGCACCGTTCGCGGGTGTCCCGTATCTCGTGAAGGATCTCGGC
GCGGCCGTGGCCGGGCTGCCGCTGTCGATGGGCAGCCGCCACTACCGCTACTTCGTGCCCGGCGACGATTCGCCGTTCAT
CGCCAAAAGCCGTGCGGCCGGGCTCAACGTGTTCGGCAAGACGAACACGTCGGAGATCGGCCAGATGCCCTACACCGAGC
CCGACCTGTTCGGCGCGTGCCGCAATCCGTGGAGCCTCGACCATACGAGCGGCGGCTCGAGCGGCGGCGCGGCGGCCGCC
GTCGCGGCCGGCGTCGTGCCGCTCGCGCATGCGTCGGACGGTGGCGGCTCGATCCGCATTCCGGCCTCGTGCTGCGGGCT
GTTCGGGCTGAAGCCGAGCCGCCATCCGGCGCTCGTCGATCTGCCGACGCACGGCGATCTCGTCGTCAATCATGCGGTGT
CGCGCAGCGTGCGCGACAGTGCGCTGCTGCTCGACGTGACGACCGGCGCGACGCTGCCGCCCGGTGCGCCGGGCACGTTC
CTCGCGGCGCTCGATACGCCGCCCCGCCCGCTGCGGATCGGCCTCGTCACCGATCCGATGCTCGCGCCGGCGCTCGCCGA
CGACACGCGCGCGGCACTCGACGACGCGGCCGCGCTCGTCGAATCGCTCGGCCATCACGTCGAACCGGCGACGCTGCATA
TCGACTACGCGCGCGCGGCCGAAACCTTCCTGACGCTGTGGGCGACGATCGCCGAGGAGATCGTGCTCGGCGCGCGCGCA
CTGACGGGCCGCACGCCGCGGCGCGGCGAGTTCGAGGCGGCGACCTGGGCGATGGCCGTGGTCGGCCGGCGGCTCGCGCG
TGCGCGACTGCCGGACGTGCTCGAGTGGCAGCGGCAGTTGACCGTGCAGGTGTCGGGTCTCGTGTCGCGCTACGACGCGA
TCCTCTGCGCCTCGCTGGCCGGCCCGCCGGTGAAGATCGGCGAACTGCAGCCGACGCCGCTCGAAGCCGCGCAGATGAAG
CTCGTCGCCGCGCTGCCCGCGAAGCCGCTGCTGCGCGAGCTGCTGGCGAAGGCGTCACAGAAGGCGTTCGCGTGGGCCGG
CTGCACCGAGCTGTTCAATCTGACGGGCCAGCCGGCGATGTCGGTGCCGCTCTACTGGAACGCGCGCGGGCTGCCGATCG
GCGTGCAGTTCGTCGCGCGACACGGCGACGACGCGCTGCTGCTGCAACTCGCGCGGCAGCTCGAGCAGGCGCGGCCGTGG
TTCGATCGCCGTCCGCCGCTGCTGCAGGCGCAGCGCTGA

Upstream 100 bases:

>100_bases
GAGCACCTGTAAGCCCGGCGCGCCGCGGGCCGGCACCGCCGCGTGCCGGGCCCGCGTTATGCTTGGTCCATCGCCATACG
TCACAACGGGGGTGACCGCG

Downstream 100 bases:

>100_bases
CGGACGAAAAAAAGCCCCGCCGGCCGAAGCCGACGGGGCATGCAGCGGCAGGCCGCGCGCGACGCTTACACGTCGAGGCG
TTCGCAGATCGCCTTGGTCG

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MHDDYLAHDAIGLATLVRERQASPLELLDAAIGQADAVNGAINAIVLQDYDAARTRAAAASAATADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPGDDSPFIAKSRAAGLNVFGKTNTSEIGQMPYTEPDLFGACRNPWSLDHTSGGSSGGAAAA
VAAGVVPLAHASDGGGSIRIPASCCGLFGLKPSRHPALVDLPTHGDLVVNHAVSRSVRDSALLLDVTTGATLPPGAPGTF
LAALDTPPRPLRIGLVTDPMLAPALADDTRAALDDAAALVESLGHHVEPATLHIDYARAAETFLTLWATIAEEIVLGARA
LTGRTPRRGEFEAATWAMAVVGRRLARARLPDVLEWQRQLTVQVSGLVSRYDAILCASLAGPPVKIGELQPTPLEAAQMK
LVAALPAKPLLRELLAKASQKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFVARHGDDALLLQLARQLEQARPW
FDRRPPLLQAQR

Sequences:

>Translated_492_residues
MHDDYLAHDAIGLATLVRERQASPLELLDAAIGQADAVNGAINAIVLQDYDAARTRAAAASAATADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPGDDSPFIAKSRAAGLNVFGKTNTSEIGQMPYTEPDLFGACRNPWSLDHTSGGSSGGAAAA
VAAGVVPLAHASDGGGSIRIPASCCGLFGLKPSRHPALVDLPTHGDLVVNHAVSRSVRDSALLLDVTTGATLPPGAPGTF
LAALDTPPRPLRIGLVTDPMLAPALADDTRAALDDAAALVESLGHHVEPATLHIDYARAAETFLTLWATIAEEIVLGARA
LTGRTPRRGEFEAATWAMAVVGRRLARARLPDVLEWQRQLTVQVSGLVSRYDAILCASLAGPPVKIGELQPTPLEAAQMK
LVAALPAKPLLRELLAKASQKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFVARHGDDALLLQLARQLEQARPW
FDRRPPLLQAQR
>Mature_492_residues
MHDDYLAHDAIGLATLVRERQASPLELLDAAIGQADAVNGAINAIVLQDYDAARTRAAAASAATADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPGDDSPFIAKSRAAGLNVFGKTNTSEIGQMPYTEPDLFGACRNPWSLDHTSGGSSGGAAAA
VAAGVVPLAHASDGGGSIRIPASCCGLFGLKPSRHPALVDLPTHGDLVVNHAVSRSVRDSALLLDVTTGATLPPGAPGTF
LAALDTPPRPLRIGLVTDPMLAPALADDTRAALDDAAALVESLGHHVEPATLHIDYARAAETFLTLWATIAEEIVLGARA
LTGRTPRRGEFEAATWAMAVVGRRLARARLPDVLEWQRQLTVQVSGLVSRYDAILCASLAGPPVKIGELQPTPLEAAQMK
LVAALPAKPLLRELLAKASQKAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFVARHGDDALLLQLARQLEQARPW
FDRRPPLLQAQR

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=507, Percent_Identity=26.232741617357, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI222831590, Length=525, Percent_Identity=24.952380952381, Blast_Score=82, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17537465, Length=519, Percent_Identity=23.3140655105973, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17556264, Length=306, Percent_Identity=27.4509803921569, Blast_Score=76, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17556276, Length=328, Percent_Identity=27.1341463414634, Blast_Score=69, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17556278, Length=327, Percent_Identity=27.217125382263, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24644968, Length=506, Percent_Identity=24.5059288537549, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI45550774, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24648435, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648437, Length=243, Percent_Identity=31.2757201646091, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21356731, Length=198, Percent_Identity=29.7979797979798, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24652985, Length=203, Percent_Identity=32.0197044334975, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922090, Length=203, Percent_Identity=32.0197044334975, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652981, Length=203, Percent_Identity=32.0197044334975, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24652983, Length=203, Percent_Identity=32.0197044334975, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24648441, Length=175, Percent_Identity=32.5714285714286, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI24648439, Length=175, Percent_Identity=32.5714285714286, Blast_Score=79, Evalue=7e-15,
Organism=Drosophila melanogaster, GI161078093, Length=234, Percent_Identity=31.1965811965812, Blast_Score=75, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 51805; Mature: 51805

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHDDYLAHDAIGLATLVRERQASPLELLDAAIGQADAVNGAINAIVLQDYDAARTRAAAA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHH
SAATADAPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPGDDSPFIAKSRAAGLNVFGK
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEECCCCCCCEEECC
TNTSEIGQMPYTEPDLFGACRNPWSLDHTSGGSSGGAAAAVAAGVVPLAHASDGGGSIRI
CCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCEEEE
PASCCGLFGLKPSRHPALVDLPTHGDLVVNHAVSRSVRDSALLLDVTTGATLPPGAPGTF
CHHHHHHHCCCCCCCCCEEECCCCCCEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCE
LAALDTPPRPLRIGLVTDPMLAPALADDTRAALDDAAALVESLGHHVEPATLHIDYARAA
EEEECCCCCCEEEEEECCCHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
ETFLTLWATIAEEIVLGARALTGRTPRRGEFEAATWAMAVVGRRLARARLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVSGLVSRYDAILCASLAGPPVKIGELQPTPLEAAQMKLVAALPAKPLLRELLAKASQ
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
KAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFVARHGDDALLLQLARQLEQARPW
HHHHHHHHHHHHCCCCCCCEECEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
FDRRPPLLQAQR
CCCCCCCCCCCC
>Mature Secondary Structure
MHDDYLAHDAIGLATLVRERQASPLELLDAAIGQADAVNGAINAIVLQDYDAARTRAAAA
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCHHHHHHHHH
SAATADAPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPGDDSPFIAKSRAAGLNVFGK
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEECCCCCCCEEECC
TNTSEIGQMPYTEPDLFGACRNPWSLDHTSGGSSGGAAAAVAAGVVPLAHASDGGGSIRI
CCCHHHCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEECCCCCCEEEE
PASCCGLFGLKPSRHPALVDLPTHGDLVVNHAVSRSVRDSALLLDVTTGATLPPGAPGTF
CHHHHHHHCCCCCCCCCEEECCCCCCEEHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCE
LAALDTPPRPLRIGLVTDPMLAPALADDTRAALDDAAALVESLGHHVEPATLHIDYARAA
EEEECCCCCCEEEEEECCCHHCCHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHH
ETFLTLWATIAEEIVLGARALTGRTPRRGEFEAATWAMAVVGRRLARARLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVSGLVSRYDAILCASLAGPPVKIGELQPTPLEAAQMKLVAALPAKPLLRELLAKASQ
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
KAFAWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFVARHGDDALLLQLARQLEQARPW
HHHHHHHHHHHHCCCCCCCEECEEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHHCCC
FDRRPPLLQAQR
CCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]