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The map label for this gene is stp [H]

Identifier: 161523310

GI number: 161523310

Start: 144073

End: 145665

Strand: Direct

Name: stp [H]

Synonym: Bmul_0130

Alternate gene names: 161523310

Gene position: 144073-145665 (Clockwise)

Preceding gene: 161523308

Following gene: 161523312

Centisome position: 4.18

GC content: 74.2

Gene sequence:

>1593_bases
ATGGATCAACCGTTTTCCGCGGTATCGCCGCGTCGGCGCGCGCTCGTGCTGTTCGCGGTCTGCGCGGCCGCCGTCGCGTT
GCCGCTGTCGTTTTCCGGCGGCGCCGTCGCGACGCCGGCGATCGGCCGCGACCTGCACGGCAGCGCGGTCGCGATGAACT
GGATCACGAACGCGTTCATGCTCGCGTTCGGCAGTTTCCTGATGGCGGCCGGCGCGCTGGCCGATCAGTTCGGCCGCAAG
CGCGTGTTCGCGGCCGGCGTCGGCGGCTTCACGCTGATGTCGGTCGCGCTCGCGTTCGCACCGTCGATACTCGCCGTCGA
CCTGCTGCGCGCGGCCCAAGGGCTCGCGGCGGCGGCCGCGCTGGCCGGCGGCACGGCCGCGCTCGCGCAGACGTTCGACG
GCGCCGAACGCACGCGTGCGTTCAGCCTGCTCGGCACGACCTTCGGGATCGGGCTCGCGTTCGGGCCGGTGACGGCCGGC
TGGCTGATCGAACACTACGGTTGGCGCGCGATCTTCGTGACGGGCGCGCTGGCCGGCGCGCTGTCGCTCGCGTTCGGGCT
GCCGCGCATGCAGGAGTCGCGCGACCCGCACGCGACGGGGCTCGACTGGCCGGGCACGGTCGCCTTCACGGCCGCGCTGA
CGCTGTTCACGTTCGGCGTGATCGAGGCGCCCGCTCGCGGCTGGACGGCGCCGCTCGTCGTCGCGCTGTTGGCCGGTGCG
GCGCTCGGCGCGGCGGCGTTCGTCGCGATCGAGACGCGCGTCGCGCGGCCGATGCTCGATCTGTCGCTGTTCCGGATTCC
GCGTTTCGTCGGCGTGCAGGTGCTGCCGATCTCGACGTGCTGCTGCTACATCGTGCTGCTCGTCGTGCTGCCGCTGCGCT
TCATCGGGATCGACGGCTTCAGCGAGATCGACGCGGGCTGGCTGATGCTCGCGATCTCCGCACCGATGCTCGTCGTGCCG
ATCGCCGCCGCGACGCTCACGCGCTGGTGGCCGGCCGGCATCGTCTCGGGGCTCGGGCTGCTCGTCGCGGCGGCCGGCCT
CGTGTGGCTCGAGGTTGCGCTGCGCGGCGGCGCGGGCCCGGCCGCGATCGCGCCGATGGTCGCGATCGGCATCGGGGCCG
GGATGCCGTGGGGGCTGATGGACGGGCTCTCGGTGAGCGTCGTGCCGAAGGAGCGCGCGGGGATGGCGACCGGCATTTTC
AGCACGACGCGCGTGGCCGGCGAGGGCATCGCGCTCGCAATCGTCGGCGCGCTGCTCGCGGCGCTCGCGCATGCGCGGCT
GCAACACACGGCGCCCGGCGCGCCGTCCGACGCCGTGCTGCACGCCGCCGCGCGGCTCGCGACCGGCGATCTCGCCGGCG
CGGCGGCCGCGCTGCCGGGCGTCGACCGCGCGGTGCTTCTCGCGAGCTACACGCACGCGTTCGACCGCCTGCTGATCGCG
CTCGCGATCGTCACCGTGCTGTGCGCGGCGATCGTGTTCGCGTTTCTCGGTGCGCGGCAGACCGCCGACGGCACGCACGC
CGACACGCGCGCGAGCCTGCCGCCGAATCGCCGCGCGCGGCGGCCGGCGTGTGCGGAATCGAACGGCCGCTGA

Upstream 100 bases:

>100_bases
CCGTTATATGCGGAGCTTCGCGTGGTTTTTCGCGGATTGGATCGGATTTATCTTGTGTCGCGTTGCCGGCGCCGATGCGC
CGGCTTTTCGAGGAGCGATG

Downstream 100 bases:

>100_bases
GCGGCGCGGCCCGTTACGCGGCGCCGCGCCCGCGTGCCCGGCGCGTCACGCGAGTACGAGCGGCGGCAGCGTCGCGTCGG
TGCGCGGCTGCGCGCACTCG

Product: major facilitator transporter

Products: NA

Alternate protein names: Spectinomycin tetracycline efflux pump [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MDQPFSAVSPRRRALVLFAVCAAAVALPLSFSGGAVATPAIGRDLHGSAVAMNWITNAFMLAFGSFLMAAGALADQFGRK
RVFAAGVGGFTLMSVALAFAPSILAVDLLRAAQGLAAAAALAGGTAALAQTFDGAERTRAFSLLGTTFGIGLAFGPVTAG
WLIEHYGWRAIFVTGALAGALSLAFGLPRMQESRDPHATGLDWPGTVAFTAALTLFTFGVIEAPARGWTAPLVVALLAGA
ALGAAAFVAIETRVARPMLDLSLFRIPRFVGVQVLPISTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPMLVVP
IAAATLTRWWPAGIVSGLGLLVAAAGLVWLEVALRGGAGPAAIAPMVAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIF
STTRVAGEGIALAIVGALLAALAHARLQHTAPGAPSDAVLHAAARLATGDLAGAAAALPGVDRAVLLASYTHAFDRLLIA
LAIVTVLCAAIVFAFLGARQTADGTHADTRASLPPNRRARRPACAESNGR

Sequences:

>Translated_530_residues
MDQPFSAVSPRRRALVLFAVCAAAVALPLSFSGGAVATPAIGRDLHGSAVAMNWITNAFMLAFGSFLMAAGALADQFGRK
RVFAAGVGGFTLMSVALAFAPSILAVDLLRAAQGLAAAAALAGGTAALAQTFDGAERTRAFSLLGTTFGIGLAFGPVTAG
WLIEHYGWRAIFVTGALAGALSLAFGLPRMQESRDPHATGLDWPGTVAFTAALTLFTFGVIEAPARGWTAPLVVALLAGA
ALGAAAFVAIETRVARPMLDLSLFRIPRFVGVQVLPISTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPMLVVP
IAAATLTRWWPAGIVSGLGLLVAAAGLVWLEVALRGGAGPAAIAPMVAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIF
STTRVAGEGIALAIVGALLAALAHARLQHTAPGAPSDAVLHAAARLATGDLAGAAAALPGVDRAVLLASYTHAFDRLLIA
LAIVTVLCAAIVFAFLGARQTADGTHADTRASLPPNRRARRPACAESNGR
>Mature_530_residues
MDQPFSAVSPRRRALVLFAVCAAAVALPLSFSGGAVATPAIGRDLHGSAVAMNWITNAFMLAFGSFLMAAGALADQFGRK
RVFAAGVGGFTLMSVALAFAPSILAVDLLRAAQGLAAAAALAGGTAALAQTFDGAERTRAFSLLGTTFGIGLAFGPVTAG
WLIEHYGWRAIFVTGALAGALSLAFGLPRMQESRDPHATGLDWPGTVAFTAALTLFTFGVIEAPARGWTAPLVVALLAGA
ALGAAAFVAIETRVARPMLDLSLFRIPRFVGVQVLPISTCCCYIVLLVVLPLRFIGIDGFSEIDAGWLMLAISAPMLVVP
IAAATLTRWWPAGIVSGLGLLVAAAGLVWLEVALRGGAGPAAIAPMVAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIF
STTRVAGEGIALAIVGALLAALAHARLQHTAPGAPSDAVLHAAARLATGDLAGAAAALPGVDRAVLLASYTHAFDRLLIA
LAIVTVLCAAIVFAFLGARQTADGTHADTRASLPPNRRARRPACAESNGR

Specific function: Contributes to spectinomycin and tetracycline resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=26.7303102625298, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=23.5148514851485, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788710, Length=284, Percent_Identity=24.2957746478873, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324954, Length=243, Percent_Identity=30.0411522633745, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 53963; Mature: 53963

Theoretical pI: Translated: 9.84; Mature: 9.84

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDQPFSAVSPRRRALVLFAVCAAAVALPLSFSGGAVATPAIGRDLHGSAVAMNWITNAFM
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHH
LAFGSFLMAAGALADQFGRKRVFAAGVGGFTLMSVALAFAPSILAVDLLRAAQGLAAAAA
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGGTAALAQTFDGAERTRAFSLLGTTFGIGLAFGPVTAGWLIEHYGWRAIFVTGALAGA
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
LSLAFGLPRMQESRDPHATGLDWPGTVAFTAALTLFTFGVIEAPARGWTAPLVVALLAGA
HHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALGAAAFVAIETRVARPMLDLSLFRIPRFVGVQVLPISTCCCYIVLLVVLPLRFIGIDGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
SEIDAGWLMLAISAPMLVVPIAAATLTRWWPAGIVSGLGLLVAAAGLVWLEVALRGGAGP
CHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AAIAPMVAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVGALLA
HHHHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
ALAHARLQHTAPGAPSDAVLHAAARLATGDLAGAAAALPGVDRAVLLASYTHAFDRLLIA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LAIVTVLCAAIVFAFLGARQTADGTHADTRASLPPNRRARRPACAESNGR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDQPFSAVSPRRRALVLFAVCAAAVALPLSFSGGAVATPAIGRDLHGSAVAMNWITNAFM
CCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHH
LAFGSFLMAAGALADQFGRKRVFAAGVGGFTLMSVALAFAPSILAVDLLRAAQGLAAAAA
HHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAGGTAALAQTFDGAERTRAFSLLGTTFGIGLAFGPVTAGWLIEHYGWRAIFVTGALAGA
HCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
LSLAFGLPRMQESRDPHATGLDWPGTVAFTAALTLFTFGVIEAPARGWTAPLVVALLAGA
HHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
ALGAAAFVAIETRVARPMLDLSLFRIPRFVGVQVLPISTCCCYIVLLVVLPLRFIGIDGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEHHHHHHHHHHHHHHHHHHHHCCCCC
SEIDAGWLMLAISAPMLVVPIAAATLTRWWPAGIVSGLGLLVAAAGLVWLEVALRGGAGP
CHHCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
AAIAPMVAIGIGAGMPWGLMDGLSVSVVPKERAGMATGIFSTTRVAGEGIALAIVGALLA
HHHHHHHHHHHCCCCCCHHHCCCEEEEECHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
ALAHARLQHTAPGAPSDAVLHAAARLATGDLAGAAAALPGVDRAVLLASYTHAFDRLLIA
HHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
LAIVTVLCAAIVFAFLGARQTADGTHADTRASLPPNRRARRPACAESNGR
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]