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The map label for this gene is alkJ [H]

Identifier: 161523262

GI number: 161523262

Start: 86400

End: 88136

Strand: Direct

Name: alkJ [H]

Synonym: Bmul_0082

Alternate gene names: 161523262

Gene position: 86400-88136 (Clockwise)

Preceding gene: 161523259

Following gene: 161523263

Centisome position: 2.51

GC content: 68.68

Gene sequence:

>1737_bases
ATGCTTTGCATCGAATCGGCGCGCACGCCGATCGACAGGAGACACATCGTGAGTACCGAACGTACGCTCGAAGGCGAATT
CGATTACGTGATCGTGGGCGCCGGCACGGCCGGCTGCGTGCTGGCCAACCGGCTGACCGAGGACCCGGACGTTCGCGTGC
TGCTGCTCGAGGCGGGCGGCAAGGACGACTATCACTGGATTCATATCCCGGTCGGCTATCTGTATTGCATCGGCAATCCG
CGCACCGACTGGCTGTACAAGACGCAGCCGGAAGCGGGGCTCAACGGCCGCGCGCTGTCGTATCCGCGCGGACGCGTGCT
CGGCGGCTGTTCGTCGATCAACGGGATGATCTACATGCGCGGCCAGCGCGACGATTACGACCGCTGGGCGCGGGAAACCG
GCGACGCCGGCTGGTCGTGGGACAGCGTGCTGCCGGTGTTCAAGCGCAGCGAGGATCATCATGCGGGCGCGAGCGACGCG
CACGGCGCGGGCGGCTACTGGCGCGTCGAGAAGCAGCGGCTGCGCTGGGAGATCCTCGAATCGTTCGCGCAGGCCGCGCA
GCAGACGGGCATTCCGGCCACCGACGATTTCAATCGCGGCGACAACACGGGTGTCGGCTACTTCGAGGTGAACCAGAAGC
GCGGCGTGCGCTGGAACACGTCGAAGGCGTTCCTGCGGCCCGCGATGGCGCGCGCGAATCTGACCGTGATCACCGGCGCG
CACGCGCAGCGCGTGATTTTCGAGGGGCGGCGTGCGATCGGCGTCGAATATCGTGGCGGCGGCACCGAGTACGTCGCGCG
CGCGCGCATCGAAGTGCTGCTCACGTCGGGCGCGGTGAATTCGCCGCAGCTGCTCGAGCTGTCCGGCATCGGCGACGGCC
GGCGGCTGCAGGCGCTCGGCATCGAGGTCGTACAGGATCTGCCGGGCGTCGGCGAAAACCTGCAGGACCACCTGCAGCTG
CGCATGGCGTTTCGCGTGCAGGGCGTGCGCACGCTGAACACGCTGTCCGCGCGCTGGTGGGGCAAGCTGATGATCGGCGC
CGAATATGCGCTGCTGCAGCGGGGGCCGATGTCGATGGCGCCGTCGCAGCTTGGCGCGTTCGCGAAATCCGATCCGGACG
ATCCGACGCTCGCGTCGCCCGATCTCGAATATCACGTGCAGCCGCTGTCGCTCGACCGCTTCGGCGAGCCGCTGCACAGC
TTCAATGCGTTCACCGCGTCCGTGTGTCATCTGCGGCCGACCTCGCGCGGCAGCGTGCATGTCGCGAGCGCCGATCCGCA
TGCGGCGCCCGTGATCGCGCCGAACTATCTGTCGACCGACTACGACCGCCACGTCGCCGCCAACGCGCTGCGTCTCACGC
GCCGCATCGCGTCCGCGCCGGCGCTCGCGCGCTACCGTCCCGAGGAAATCCTGCCGGGCACGCGCTATCAGACCGAAGCC
GAGCTGCAGGAAGCGGCCGGCGCGGTCGGCACGACGATCTTCCATCCGGTTGGCACGTGCCGCATGGGACGCGCGGACGA
TCCGCGCGCGGTCGTCGACAGCCGGCTGCGCGTGCGCGGCATCGCGGGGCTGCGCATCGTCGATGCGTCGGTGATGCCGT
TCATCACGTCGGGCAACACGAATTCGCCGACGCTGATGATCGCCGAGCGCGCGAGTGACATGATTCGCGCGGACCGCCGT
GCAGCGCGCGACGCAGCCGCCTCCGTGCACACCGACGCGGTCGTGACGCCCGCATGA

Upstream 100 bases:

>100_bases
CCGACGTGCAGCACGCCATGCGCCGCGGGCACCGCAGGCACCGCAGGCGCACTATGCTGAAAACGACGCGCGGAATCGCG
CCGCGCGCCGCGATAACAAC

Downstream 100 bases:

>100_bases
CGACACGCTGACGCGCGCGTCGGCATCCATTGCGCCGCGATCGCGGACGTGCGTCGCGCCACGCGCTGCTTCGCGCTGCG
AAACAACGCATGCTTGTGCG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MLCIESARTPIDRRHIVSTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVRVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAGLNGRALSYPRGRVLGGCSSINGMIYMRGQRDDYDRWARETGDAGWSWDSVLPVFKRSEDHHAGASDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNTSKAFLRPAMARANLTVITGA
HAQRVIFEGRRAIGVEYRGGGTEYVARARIEVLLTSGAVNSPQLLELSGIGDGRRLQALGIEVVQDLPGVGENLQDHLQL
RMAFRVQGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPTLASPDLEYHVQPLSLDRFGEPLHS
FNAFTASVCHLRPTSRGSVHVASADPHAAPVIAPNYLSTDYDRHVAANALRLTRRIASAPALARYRPEEILPGTRYQTEA
ELQEAAGAVGTTIFHPVGTCRMGRADDPRAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AARDAAASVHTDAVVTPA

Sequences:

>Translated_578_residues
MLCIESARTPIDRRHIVSTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVRVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAGLNGRALSYPRGRVLGGCSSINGMIYMRGQRDDYDRWARETGDAGWSWDSVLPVFKRSEDHHAGASDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNTSKAFLRPAMARANLTVITGA
HAQRVIFEGRRAIGVEYRGGGTEYVARARIEVLLTSGAVNSPQLLELSGIGDGRRLQALGIEVVQDLPGVGENLQDHLQL
RMAFRVQGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPTLASPDLEYHVQPLSLDRFGEPLHS
FNAFTASVCHLRPTSRGSVHVASADPHAAPVIAPNYLSTDYDRHVAANALRLTRRIASAPALARYRPEEILPGTRYQTEA
ELQEAAGAVGTTIFHPVGTCRMGRADDPRAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AARDAAASVHTDAVVTPA
>Mature_578_residues
MLCIESARTPIDRRHIVSTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVRVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAGLNGRALSYPRGRVLGGCSSINGMIYMRGQRDDYDRWARETGDAGWSWDSVLPVFKRSEDHHAGASDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNTSKAFLRPAMARANLTVITGA
HAQRVIFEGRRAIGVEYRGGGTEYVARARIEVLLTSGAVNSPQLLELSGIGDGRRLQALGIEVVQDLPGVGENLQDHLQL
RMAFRVQGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPTLASPDLEYHVQPLSLDRFGEPLHS
FNAFTASVCHLRPTSRGSVHVASADPHAAPVIAPNYLSTDYDRHVAANALRLTRRIASAPALARYRPEEILPGTRYQTEA
ELQEAAGAVGTTIFHPVGTCRMGRADDPRAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AARDAAASVHTDAVVTPA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=538, Percent_Identity=42.9368029739777, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1786503, Length=549, Percent_Identity=39.8907103825137, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17532301, Length=565, Percent_Identity=35.2212389380531, Blast_Score=344, Evalue=7e-95,
Organism=Drosophila melanogaster, GI45549471, Length=597, Percent_Identity=36.6834170854271, Blast_Score=345, Evalue=4e-95,
Organism=Drosophila melanogaster, GI24642055, Length=594, Percent_Identity=36.5319865319865, Blast_Score=343, Evalue=1e-94,
Organism=Drosophila melanogaster, GI45551458, Length=596, Percent_Identity=36.5771812080537, Blast_Score=342, Evalue=3e-94,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=36.3478260869565, Blast_Score=339, Evalue=3e-93,
Organism=Drosophila melanogaster, GI24642059, Length=568, Percent_Identity=36.2676056338028, Blast_Score=318, Evalue=9e-87,
Organism=Drosophila melanogaster, GI24642048, Length=575, Percent_Identity=35.1304347826087, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI18859995, Length=587, Percent_Identity=34.7529812606474, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24650267, Length=593, Percent_Identity=34.7386172006745, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24642039, Length=577, Percent_Identity=35.1819757365685, Blast_Score=285, Evalue=8e-77,
Organism=Drosophila melanogaster, GI17137792, Length=567, Percent_Identity=33.8624338624339, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI24642035, Length=577, Percent_Identity=33.4488734835355, Blast_Score=281, Evalue=8e-76,
Organism=Drosophila melanogaster, GI18859993, Length=589, Percent_Identity=31.918505942275, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24642037, Length=576, Percent_Identity=32.1180555555556, Blast_Score=265, Evalue=7e-71,
Organism=Drosophila melanogaster, GI24642051, Length=573, Percent_Identity=31.413612565445, Blast_Score=254, Evalue=1e-67,
Organism=Drosophila melanogaster, GI24642057, Length=601, Percent_Identity=29.1181364392679, Blast_Score=163, Evalue=3e-40,
Organism=Drosophila melanogaster, GI24645930, Length=592, Percent_Identity=27.8716216216216, Blast_Score=152, Evalue=8e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 63314; Mature: 63314

Theoretical pI: Translated: 7.93; Mature: 7.93

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCIESARTPIDRRHIVSTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVRVLLLEAGG
CEECCCCCCCCCHHHEECCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
KDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAGLNGRALSYPRGRVLGGCSSINGMIYMR
CCCEEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCCEECCCCCCEEECCCCCCCEEEEE
GQRDDYDRWARETGDAGWSWDSVLPVFKRSEDHHAGASDAHGAGGYWRVEKQRLRWEILE
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
SFAQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNTSKAFLRPAMARANLTVITGA
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHCCEEEEECC
HAQRVIFEGRRAIGVEYRGGGTEYVARARIEVLLTSGAVNSPQLLELSGIGDGRRLQALG
CHHHHHHCCCEEEEEEECCCCHHHHHHEEEEEEEECCCCCCCCEEEEECCCCCCEEHHHH
IEVVQDLPGVGENLQDHLQLRMAFRVQGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
PSQLGAFAKSDPDDPTLASPDLEYHVQPLSLDRFGEPLHSFNAFTASVCHLRPTSRGSVH
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCHHHHHCHHHEEEEEECCCCCCCEE
VASADPHAAPVIAPNYLSTDYDRHVAANALRLTRRIASAPALARYRPEEILPGTRYQTEA
EECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCHHH
ELQEAAGAVGTTIFHPVGTCRMGRADDPRAVVDSRLRVRGIAGLRIVDASVMPFITSGNT
HHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEECCCC
NSPTLMIAERASDMIRADRRAARDAAASVHTDAVVTPA
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MLCIESARTPIDRRHIVSTERTLEGEFDYVIVGAGTAGCVLANRLTEDPDVRVLLLEAGG
CEECCCCCCCCCHHHEECCCCCCCCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
KDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAGLNGRALSYPRGRVLGGCSSINGMIYMR
CCCEEEEEEEEEEEEECCCCCCCCEEECCCCCCCCCCEECCCCCCEEECCCCCCCEEEEE
GQRDDYDRWARETGDAGWSWDSVLPVFKRSEDHHAGASDAHGAGGYWRVEKQRLRWEILE
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
SFAQAAQQTGIPATDDFNRGDNTGVGYFEVNQKRGVRWNTSKAFLRPAMARANLTVITGA
HHHHHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCEECCCHHHHHHHHHHCCEEEEECC
HAQRVIFEGRRAIGVEYRGGGTEYVARARIEVLLTSGAVNSPQLLELSGIGDGRRLQALG
CHHHHHHCCCEEEEEEECCCCHHHHHHEEEEEEEECCCCCCCCEEEEECCCCCCEEHHHH
IEVVQDLPGVGENLQDHLQLRMAFRVQGVRTLNTLSARWWGKLMIGAEYALLQRGPMSMA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
PSQLGAFAKSDPDDPTLASPDLEYHVQPLSLDRFGEPLHSFNAFTASVCHLRPTSRGSVH
HHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCCHHHHHCHHHEEEEEECCCCCCCEE
VASADPHAAPVIAPNYLSTDYDRHVAANALRLTRRIASAPALARYRPEEILPGTRYQTEA
EECCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHCCCCCCCCHHH
ELQEAAGAVGTTIFHPVGTCRMGRADDPRAVVDSRLRVRGIAGLRIVDASVMPFITSGNT
HHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHCCCEEEECCCEEEECCCCCEEEECCCC
NSPTLMIAERASDMIRADRRAARDAAASVHTDAVVTPA
CCCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]