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The map label for this gene is splF [H]

Identifier: 161510024

GI number: 161510024

Start: 1939504

End: 1940223

Strand: Reverse

Name: splF [H]

Synonym: USA300HOU_1801

Alternate gene names: 161510024

Gene position: 1940223-1939504 (Counterclockwise)

Preceding gene: 161510025

Following gene: 161510023

Centisome position: 67.53

GC content: 33.06

Gene sequence:

>720_bases
ATGAATAAAAATATAATCATCAAAAGTATTGCAGCATTGACGATTTTAACATCAATAACTGGTGTCGGCACAACAATGGT
TGAAGGTATTCAACAAACAGCCAAAGCCGAAAATACTGTTAAACAAATTACAAATACAAATGTTGCACCATACAGTGGTG
TTACATGGATGGGCGCTGGAACAGGATTTGTAGTTGGAAATCATACAATCATTACCAATAAACATGTTACCTATCACATG
AAAGTCGGTGATGAAATCAAAGCACATCCTAATGGTTTTTATAATAACGGTGGTGGACTTTATAAAGTTACTAAGATTGT
AGATTATCCTGGTAAAGAAGATATTGCGGTTGTACAAGTTGAAGAAAAATCAACACAACCAAAAGGTAGAAAATTCAAAG
ATTTCACTAGTAAATTTAATATAGCATCAGAAGCTAAAGAAAATGAACCTATATCAGTCATTGGTTATCCAAATCCTAAT
GGAAATAAACTACAAATGTATGAATCAACTGGTAAAGTATTATCAGTGAATGGGAATATAGTGTCATCGGATGCAATTAT
TCAGCCTGGTAGCTCTGGTTCACCTATATTAAATAGTAAACACGAAGCTATTGGTGTAATCTATGCCGGTAATAAGCCAT
CAGGTGAAAGCACAAGAGGATTTGCTGTTTATTTCTCTCCTGAAATTAAGAAATTCATTGCAGATAATTTAGATAAATAA

Upstream 100 bases:

>100_bases
CAAATCATCTCTGTGATTCCATTTATTCGAAATGATTAAAAAAAATAAAACTTCAAAAAGCTAACATTATAATTATACAA
ATACTTAGAGGAGCAGAAAA

Downstream 100 bases:

>100_bases
TTAAAACTTAGACATTCACCCAATCCTGACAAAATATACTATAACTAACATTTATTAATATATATTGCATTATTTAATAT
GCATCAAAGCCAATCAACGA

Product: serine protease SplF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHM
KVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK

Sequences:

>Translated_239_residues
MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHM
KVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK
>Mature_239_residues
MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAGTGFVVGNHTIITNKHVTYHM
KVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQVEEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPN
GNKLQMYESTGKVLSVNGNIVSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK

Specific function: Unknown

COG id: COG3591

COG function: function code E; V8-like Glu-specific endopeptidase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S1B family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009003
- InterPro:   IPR000126
- InterPro:   IPR001254
- InterPro:   IPR008256 [H]

Pfam domain/function: PF00089 Trypsin [H]

EC number: NA

Molecular weight: Translated: 25655; Mature: 25655

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS00672 V8_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAG
CCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
TGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQV
CEEEEECEEEEECCEEEEEEEECCEEECCCCCEEECCCCEEEEEEEEECCCCCCEEEEEE
EEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNI
ECCCCCCCCCCHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCE
VSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK
EECCCEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCC
>Mature Secondary Structure
MNKNIIIKSIAALTILTSITGVGTTMVEGIQQTAKAENTVKQITNTNVAPYSGVTWMGAG
CCCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCC
TGFVVGNHTIITNKHVTYHMKVGDEIKAHPNGFYNNGGGLYKVTKIVDYPGKEDIAVVQV
CEEEEECEEEEECCEEEEEEEECCEEECCCCCEEECCCCEEEEEEEEECCCCCCEEEEEE
EEKSTQPKGRKFKDFTSKFNIASEAKENEPISVIGYPNPNGNKLQMYESTGKVLSVNGNI
ECCCCCCCCCCHHHHHHHCCCHHHCCCCCCEEEEECCCCCCCEEEEEECCCCEEEECCCE
VSSDAIIQPGSSGSPILNSKHEAIGVIYAGNKPSGESTRGFAVYFSPEIKKFIADNLDK
EECCCEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEEEEECHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA