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The map label for this gene is nagP [H]

Identifier: 161509942

GI number: 161509942

Start: 1838303

End: 1839769

Strand: Reverse

Name: nagP [H]

Synonym: USA300HOU_1715

Alternate gene names: 161509942

Gene position: 1839769-1838303 (Counterclockwise)

Preceding gene: 161509944

Following gene: 161509941

Centisome position: 64.04

GC content: 34.49

Gene sequence:

>1467_bases
ATGTATAAATTTTTCCAAAATTTAGGGCGTTCGTTAATGTTACCAGTTGCCATTTTACCGGCTGCAGCAATTATTGCCGG
AATTGGTAATACGTTAAATGCACTTCATGCGACGCCGAAAATCGCAATGTTCTTTACTACGGTAGGTACTACGATTTTAG
AGCAATTAGGTATTTTATTTGCAATTGGTGTAGCTATAGGAATGGCAAAGAAAAATGATGGTGTAGTCGCGCTTGCAGCT
ACGTTAGGATATTTTTTAGTTACAGTTGTATTATCACCAATGAAATTAGCGCCTTTATTAGGAATGAAAGCTTCGGAAAT
AAATTCAGCTTTTGAAAAGATGAATAATGGCAATGTCTTTGTTGGTATTGTAATAGGTTTAATAGCAGCTTATGCATACA
ATAAGTTTAGTGAAACAGAATTACCATTAGCATTATCATTTTTTAGTGGTAAACGTCTAGTTCCAATTATGACTGCATTT
TACTGTACATTTTTAGTTGTCATATTGTTATTCTTATGGCCACTACTTTATTCATGGATTGTAAAATTTGGTGAATCTAT
AGTCGGTTTAGGATCGTTTGGAGCTTTCATATATGGTGTTGCCAATAGATTATTAATTCCTACAGGATTACATCATGCAC
TGAATAGTGTGTTCTGGTTTGATACGATTGGTATTAATGATATTGGTAAATTCCAAAGTGGCAAAGATGCTATAAAAGGT
ATTACAGGACGCTATCAAGCGGGATTCTTCCCAATCATGATGTTCGGTATTCCAGCGGCTGCGTTAGCAATGTATCATAC
AGCTAAAACTACTCAGAAAAAACAAGTTTACGGTTGGTTCCTAGCGAGTTCAGTTGCAGCATTTTTCGTTGGTGTTACAG
AGCCGATTGAGTTTGCATTTATGTTTGTTGCGCCAATCTTATATGTTGTTCATGCGTTATTAACAGGATTATCTTTATTT
ATCGCAGCTACATTCCATTGGACAGCTGGATTTTCATTTAGTGCAGGTTTGATAGATTATGTGTTATCACTAATTAATCC
AGTATCAAATCATCCATTGATGTTGCTTGTTCAAGGTGTTGTATTTTTCATCTTATACTATGTGATTTTCCGTGTAGTAA
TCCAAGTATTTAACTTGAATACGATTGGTAGAGGTGAAAATGAATTAGTTGACCCAACAGTTGTAAAAGATAATATTGCT
CCTGGTGAAAATGATATTAAACAAAGTAAATATCATCAACATGCTATACAAATATTAGAAGGTTTAGGCGGTCAAGAGAA
TATTGTTAATTTAACCAATTGTGCAACAAGGTTGCGTCTAGAGTTAAAAGACACATCTATTATTGATAAACAGAAGATTA
AAAACGCCGGTGCAGTTGGTGTTACTGTTAATGGCAAACATTCAACTCAAGTTATTGTCGGTACACACGTACAGCAGGTT
GCTGATGAAATTGAAAAACATTTATAA

Upstream 100 bases:

>100_bases
TTAGTAAGCCTAGGTGACTATAACGGAGTATCGAAGTAGTTATTCTAGTTACATTAGTAATCTTTATATTTCTACATTAT
TTTAAGAAGGATGACATTCA

Downstream 100 bases:

>100_bases
AATTTAAACAGCTAAAGCGCAAATCTAACTTCAATAGGGTTTGCGCTTTTTTTAGTCAAATAGTAGAATGATTTATATCT
GAATTTTCAGAAATGGAGTG

Product: PTS family porter component IIBC

Products: NA

Alternate protein names: EIICB-Nag; N-acetylglucosamine permease IIC component; PTS system N-acetylglucosamine-specific EIIC component; N-acetylglucosamine-specific phosphotransferase enzyme IIB component; PTS system N-acetylglucosamine-specific EIIB component [H]

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAA
TLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKG
ITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLF
IAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIA
PGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQV
ADEIEKHL

Sequences:

>Translated_488_residues
MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAA
TLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKG
ITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLF
IAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIA
PGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQV
ADEIEKHL
>Mature_488_residues
MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILFAIGVAIGMAKKNDGVVALAA
TLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKG
ITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLF
IAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIA
PGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQV
ADEIEKHL

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1263

COG function: function code G; Phosphotransferase system IIC components, glucose/maltose/N-acetylglucosamine-specific

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1786894, Length=498, Percent_Identity=41.566265060241, Blast_Score=315, Evalue=6e-87,
Organism=Escherichia coli, GI1787343, Length=494, Percent_Identity=36.0323886639676, Blast_Score=277, Evalue=1e-75,
Organism=Escherichia coli, GI1787908, Length=523, Percent_Identity=34.0344168260038, Blast_Score=271, Evalue=9e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018113
- InterPro:   IPR001996
- InterPro:   IPR003352
- InterPro:   IPR013013
- InterPro:   IPR011535
- InterPro:   IPR010974 [H]

Pfam domain/function: PF00367 PTS_EIIB; PF02378 PTS_EIIC [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 53115; Mature: 53115

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: PS01035 PTS_EIIB_TYPE_1_CYS ; PS51098 PTS_EIIB_TYPE_1 ; PS51103 PTS_EIIC_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILF
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVF
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
VGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAFYCTFLVVILLFLWPLLYSWI
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKG
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
ITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAF
CCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MFVAPILYVVHALLTGLSLFIAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
VFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIAPGENDIKQSKYHQHAIQILE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
GLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQV
CCCCCCCEEEHHHHHHHHEEEECCCHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHH
ADEIEKHL
HHHHHHCC
>Mature Secondary Structure
MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMFFTTVGTTILEQLGILF
CCHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
AIGVAIGMAKKNDGVVALAATLGYFLVTVVLSPMKLAPLLGMKASEINSAFEKMNNGNVF
HHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEE
VGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAFYCTFLVVILLFLWPLLYSWI
HHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVFWFDTIGINDIGKFQSGKDAIKG
HHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHC
ITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQVYGWFLASSVAAFFVGVTEPIEFAF
CCCCCCCCHHHHHHHHCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
MFVAPILYVVHALLTGLSLFIAATFHWTAGFSFSAGLIDYVLSLINPVSNHPLMLLVQGV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHH
VFFILYYVIFRVVIQVFNLNTIGRGENELVDPTVVKDNIAPGENDIKQSKYHQHAIQILE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHH
GLGGQENIVNLTNCATRLRLELKDTSIIDKQKIKNAGAVGVTVNGKHSTQVIVGTHVQQV
CCCCCCCEEEHHHHHHHHEEEECCCHHHHHHHHCCCCCEEEEECCCCCCEEEEHHHHHHH
ADEIEKHL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9272861; 9384377 [H]