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The map label for this gene is rsmE [H]

Identifier: 161509805

GI number: 161509805

Start: 1687030

End: 1687791

Strand: Reverse

Name: rsmE [H]

Synonym: USA300HOU_1578

Alternate gene names: 161509805

Gene position: 1687791-1687030 (Counterclockwise)

Preceding gene: 161509806

Following gene: 161509804

Centisome position: 58.75

GC content: 31.5

Gene sequence:

>762_bases
GTGAATAATGTGCAACGTTATTTCATAGACCAAAACGCTGATGTAAGTCAGCGTTTTTTTATTACAAAAAAAGAAGATAT
TCATCATATAACAAATGTAATGAGATATGATGTTGGTAGTAAAATTATTTTAACTTTTAATGATCAAACAGTTTATTTAT
GTGAAATTGCTGACATAACAAGTGATAAAATTGAAGTTGATTTAAAAGAAAAACAAAATATTAATACAGAATTGCCAGTT
GATGTTACGATTTGCAGTGGACTAATCAAAGCTGACAAATATGAGTGGTTGCTACAAAAAGCTACTGAATTGGGTGCTTC
ATCATTTATAGCTGTGAGCATGGAACGTTCAATTGTTAAATTAAACGAGGCAAAAGTTGCCAAAAAGATAGAGCGTTGGC
AAAAAATAATTAAAGAAGCTGCTGAACAAAGCTATCGTTTAGTGATTCCATCTATTCAATTCGAGTCGAATTTAAAATTA
ATTTGTGATACTATTGATAATTATGACTATATTCTTATTGCATATGAAGAGGAAGCAAAAGATGGTGAGTTAAGCAATTT
CAAGCAAACTTTACAACAATTCAATGCTCAGGATAAAGTGTTGATGATTTTTGGTCCTGAGGGTGGTTTGTCAGAAAATG
AAATTTCATTATTTAGTAATACTAGTACAGTTGTTGGTCTTGGGCCGAGAATTTTACGGGCAGAAACTGCGCCATTGTAT
GCGTTAAGTGCAATAAGTTATGAAAAAGAATTAATGGGGTGA

Upstream 100 bases:

>100_bases
AAGTATGAAGGTATACAGTCACATATGGAGCGTGTAGGTTTTAAAATTATTTCAGAACAACATGACAACGGTTGGGTTTG
TCTTGTTGGCCAGAAAGTGA

Downstream 100 bases:

>100_bases
ATATTTATGTCAACAGTTGCGTTTCACACATTAGGTTGTAAAGTAAACCATTATGAAACTGAAGCAATCTGGCAATTATT
TAAAGAAGCAAACTATGAGC

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MNNVQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPV
DVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKL
ICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLY
ALSAISYEKELMG

Sequences:

>Translated_253_residues
MNNVQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPV
DVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKL
ICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLY
ALSAISYEKELMG
>Mature_253_residues
MNNVQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADITSDKIEVDLKEKQNINTELPV
DVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKL
ICDTIDNYDYILIAYEEEAKDGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLY
ALSAISYEKELMG

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=218, Percent_Identity=32.1100917431193, Blast_Score=100, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28797; Mature: 28797

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNVQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADIT
CCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCC
SDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVK
CCEEEEEEHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
LNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAK
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEECCCCEEEEEECCCCCEEEEEEECCCC
DGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLY
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCCEEEECCCCEE
ALSAISYEKELMG
EEHHHHHHHHHCC
>Mature Secondary Structure
MNNVQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFNDQTVYLCEIADIT
CCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEEECCC
SDKIEVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELGASSFIAVSMERSIVK
CCEEEEEEHHCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHH
LNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICDTIDNYDYILIAYEEEAK
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECEEECCCCEEEEEECCCCCEEEEEEECCCC
DGELSNFKQTLQQFNAQDKVLMIFGPEGGLSENEISLFSNTSTVVGLGPRILRAETAPLY
CCCHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECCCEEEECCCCEEEECCCCEE
ALSAISYEKELMG
EEHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9023197; 9384377 [H]