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The map label for this gene is norB [H]

Identifier: 161508538

GI number: 161508538

Start: 319310

End: 320695

Strand: Reverse

Name: norB [H]

Synonym: USA300HOU_0287

Alternate gene names: 161508538

Gene position: 320695-319310 (Counterclockwise)

Preceding gene: 161508541

Following gene: 161508534

Centisome position: 11.16

GC content: 37.52

Gene sequence:

>1386_bases
GTGACATCTGTGAATAACACACAATCTTCACCACGCAGTAATATTATTATTGCGATTATGTTGTCTGCATTAACATATTG
GTTGTTTGCACAATCATTTATTAATATAGGACCTCTCGTTGGTCAAACGTATCAAACCTCTCCTGCCGTGTTAAATTTAT
CTATTAGTTTAACTTCCTTCGCCACAGGTATCTTCATGGTGGCTGCAGGTGATATTGCTGATAAAATAGGACAACTGAGA
ATGACATACATGGGTCTCATAATCAGTATGTTTGCATCTCTTCTATTAATTATATCGGACATCACTGCACTGCTCATCAT
CGGTAGAATTTTACAAGGTCTATCAGCAGCTATCTTGTTACCTTCAACAGTTGGCGTGTTAAATAATCAATTTAAAGGAG
AACATTTAAGACGAGCGATTAGTTATCTAATGATTAGTACTGTTGGTGGCATCGGCCTAGCTGGTGTTATCGGCGGTTTA
ATTGCCTCAAATTTCGGATGGCAAACGAATTTCATCATTAGTATAGTCATTGCTTTCATTGCCATATTGCTTCTAAAAGG
CACACCTGAAAAAGTAAGTCAACATAGCCACCGTCATCCATTCGATTACAAAGGTATGTCGATTTTCGCTGTTATGATTG
GTAGCTTTACATTATTGTTAACACAAGGATTCGAACAAGGTTGGTTTAGTACATTTTCAATCATTTGTCTGAGCATTTTT
ATCATCACTACGTTGATATTCATCATCATCGAACGTCGACATGAAGTACCTTTTATTGATTTCTCAGTATTACGCAACCG
TCCGTTCATTGGTGCATTTTTAAATAACTTTGTTTTAAATAGCGGTCTAGGCGTAACAGTGGTCTTTTTCATATATGCTC
AAACACACCTTGGTTTATCAGCTGCGCAATCTGGACTTGTTACATTGCCATATGCCATTGTGGCAGTTGCGATGATTCGT
TTAGGTGAAAAAGCAACATTACGTTTCGGTGGCAAATTGATGCTCATCATTGGTCCGTTGTTCCCTGTCATCGGCATCAC
TATTATTAGCATGACTCAATTATTGGCATCACAATATGTCATTGCAGTTATCATTGGTTTCGTCATATGTGCGATAGGTA
ATGGTTTAGTCGCAACACCTGGACTTACGATTGCAATTTTCAGTATGCCTAATGAAAAAGTTGGTTTAGCTACAGGATTA
TATAAAATGAGTGGTACATTAGGTGGCGCCTTTGGTATAGCACTAAGTACTACAGTTTTCAGTATGTTACAACTAAACTA
TGCACCAAGTGTAGCTGCAACCGTAACATTTATAGTCAGCATTGTATTGATGATCCTTGGCTCATTGTCTGCATACATGA
TCATTCCAAAAACAGTTAAATCTTAA

Upstream 100 bases:

>100_bases
TATGATATGTAAGGAAATCATGACTTATGTGTGAGTGAACGATCATCTATACATCCGTTCACTTCATCTCATGACTTTCT
ATATTTAATTTTTACAAGGA

Downstream 100 bases:

>100_bases
ATATAATAGAAGAATTATGTTTCGAAATATCTTTATCACTTTAAAATGATATACAAGAAATCCAAGAAAAATAAGCGAAC
TGAATAAATAAAGATTCAAT

Product: major facilitator transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MTSVNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLR
MTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGL
IASNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIF
IITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIR
LGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGL
YKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Sequences:

>Translated_461_residues
MTSVNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLR
MTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGL
IASNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIF
IITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIR
LGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGL
YKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
>Mature_460_residues
TSVNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSFATGIFMVAAGDIADKIGQLRM
TYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLI
ASNFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIFI
ITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRL
GEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLY
KMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS

Specific function: Multidrug efflux pump that acts independently of norA and is one of the factors that confers resistance against diverse quinolones and chemical compounds [H]

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=374, Percent_Identity=24.5989304812834, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI1788710, Length=407, Percent_Identity=24.0786240786241, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI87081983, Length=354, Percent_Identity=27.683615819209, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790195, Length=302, Percent_Identity=22.8476821192053, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49329; Mature: 49198

Theoretical pI: Translated: 10.17; Mature: 10.17

Prosite motif: PS00962 RIBOSOMAL_S2_1 ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHH
ATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALLIIGRILQGLSAAILL
HHHHHHEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIASNFGWQTNFIISIVIAFI
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
AILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLTQGFEQGWFSTFSIICLSIF
HHHHHCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
IITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVLNSGLGVTVVFFIYAQTHLGLS
HHHHHHHHHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHH
AAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLIIGPLFPVIGITIISMTQLLASQYV
HHCCCCCHHHHHHHHHHHHHCCCCCEEEECCEEEEHHHHHHHHHHHHHHHHHHHHHHHHH
IAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVGLATGLYKMSGTLGGAFGIALSTTVF
HHHHHHHHHHHHCCCEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHH
SMLQLNYAPSVAATVTFIVSIVLMILGSLSAYMIIPKTVKS
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC
>Mature Secondary Structure 
TSVNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTSPAVLNLSISLTSF
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEHHHH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HHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHEEEECHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA