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The map label for this gene is sirA [H]

Identifier: 161508383

GI number: 161508383

Start: 133135

End: 134109

Strand: Reverse

Name: sirA [H]

Synonym: USA300HOU_0126

Alternate gene names: 161508383

Gene position: 134109-133135 (Counterclockwise)

Preceding gene: 161508404

Following gene: 161508382

Centisome position: 4.67

GC content: 32.62

Gene sequence:

>975_bases
ATGCTTGTTGTTACGCTTGCTTTCCTACTTGTTTTAGCAGGATGTAGTGGGAATTCAAATAAACAATCATCTGATAACAA
AGATAAGGAAACAACTTCAATTAAACATGCAATGGGTACAACTGAAATTAAAGGGAAACCAAAGCGTGTTGTTACGCTAT
ATCAAGGTGCCACTGACGTCGCTGTATCTTTAGGTGTTAAACCTGTAGGTGCTGTAGAATCATGGACACAAAAACCGAAA
TTCGAATACATAAAAAATGATTTAAAAGATACTAAGATTGTAGGTCAAGAACCTGCACCTAACTTAGAGGAAATCTCTAA
ATTAAAACCGGACTTAATTGTCGCGTCAAAAGTTAGAAATGAAAAAGTTTACGATCAATTATCTAAAATCGCACCAACAG
TTTCTACTGATACAGTTTTCAAATTCAAAGATACAACTAAGTTAATGGGGAAAGCTTTAGGGAAAGAAAAAGAAGCTGAA
GATTTACTTAAAAAGTACGATGATAAAGTAGCTGCATTCCAAAAAGATGCAAAAGCAAAGTATAAAGATGCATGGCCATT
GAAAGCTTCAGTTGTTAACTTCCGTGCTGATCATACAAGAATTTATGCTGGTGGATATGCTGGTGAAATCTTAAATGATT
TAGGATTCAAACGTAATAAAGACTTACAAAAACAAGTTGATAATGGTAAAGATATTATCCAACTTACATCTAAAGAAAGC
ATTCCATTAATGAACGCTGATCATATTTTTGTAGTAAAATCAGATCCAAATGCGAAAGATGCTGCATTAGTTAAAAAGAC
TGAAAGCGAATGGACTTCAAGTAAAGAGTGGAAAAATTTAGACGCAGTTAAAAACAACCAAGTATCTGATGATTTAGATG
AAATCACTTGGAACTTAGCTGGCGGATATAAATCTTCATTAAAACTTATTGACGATTTATATGAAAAGTTAAATATTGAA
AAACAATCAAAATAA

Upstream 100 bases:

>100_bases
TGACTTAGGAAAAAATTTAATTCATACTAAATCGTGATAATGATTCTCATTGTCATACATCACGAAGGAGGCTAATTAGT
CAATGAATAAAGTAATTAAA

Downstream 100 bases:

>100_bases
TTAAGGAGTTTTACGATGCTACTTAAACCAAAATACCAAATCGTTATTGCTGGTTTATGTCTTGCAATAGTAGCTATCTT
AAGTTTAATGATTGGAAATA

Product: iron (Fe3+) ABC transporter binding protein

Products: ADP; phosphate; iron dicitrate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPK
FEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAE
DLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKES
IPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIE
KQSK

Sequences:

>Translated_324_residues
MLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPK
FEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAE
DLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKES
IPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIE
KQSK
>Mature_324_residues
MLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPK
FEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAE
DLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKES
IPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIE
KQSK

Specific function: Part of the ABC transporter complex yfiYZ/yfhA/yusV involved in import of the iron-hydroxamate siderophores schizokinen, arthrobactin and corprogen. Binds the siderophores and delivers them to the surface of yfiZ/yfhA (Probable) [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable). Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082411, Length=283, Percent_Identity=22.2614840989399, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36030; Mature: 36030

Theoretical pI: Translated: 9.61; Mature: 9.61

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDV
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
AVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRN
HHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCEEECCCCCCCHHHHHHCCCCCEEHHHHHH
EKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQKDAKAK
HHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
YKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKES
CCCCCCCHHHHEEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCC
IPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLA
CCEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCHHHHHHHHC
GGYKSSLKLIDDLYEKLNIEKQSK
CCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAMGTTEIKGKPKRVVTLYQGATDV
CHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEEEEECCCHHH
AVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQEPAPNLEEISKLKPDLIVASKVRN
HHCCCCCCCCHHHHHHCCCCHHHHHHHHHHCEEECCCCCCCHHHHHHCCCCCEEHHHHHH
EKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALGKEKEAEDLLKKYDDKVAAFQKDAKAK
HHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
YKDAWPLKASVVNFRADHTRIYAGGYAGEILNDLGFKRNKDLQKQVDNGKDIIQLTSKES
CCCCCCCHHHHEEEECCCEEEEECCHHHHHHHHHCCCCCCCHHHHHCCCCCEEEECCCCC
IPLMNADHIFVVKSDPNAKDAALVKKTESEWTSSKEWKNLDAVKNNQVSDDLDEITWNLA
CCEECCCEEEEEECCCCCCHHHHHHHHHHHHCCCHHHCCCHHHCCCCCCCCHHHHHHHHC
GGYKSSLKLIDDLYEKLNIEKQSK
CCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; iron dicitrate [Periplasm]; H2O [C]

Specific reaction: ATP + iron dicitrate [Periplasm] + H2O = ADP + phosphate + iron dicitrate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8946165; 9384377 [H]