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The map label for this gene is arcB1

Identifier: 161508331

GI number: 161508331

Start: 69785

End: 70783

Strand: Reverse

Name: arcB1

Synonym: USA300HOU_0067

Alternate gene names: 161508331

Gene position: 70783-69785 (Counterclockwise)

Preceding gene: 161508332

Following gene: 161508330

Centisome position: 2.46

GC content: 33.63

Gene sequence:

>999_bases
ATGAAAAATTTAAGAAATAGAAACTTTTTAACTTTGTTAGACTTCACACAAAAAGAAATGGAATTTTTACTTAATTTATC
TGAAGATCTTAAACGCGCAAAATATGCAGGAATAGAACAACAAAAAATGAAAGGTAAAAATATCGCTCTACTTTTTGAAA
AAGATTCAACACGCACTCGATGTGCATTTGAAACAGCGGCTTATGATCAAGGTGCACATGTAACATACCTTGGGCCAACA
GGTTCTCAAATGGGTAAAAAAGAGTCTACCAAAGATACTGCTCGTGTTTTAGGTGGAATGTATGATGGCATAGAATATCG
TGGATTCTCACAAAGAGTAGTTGAGGATTTGGCAAAATATTCTGGCGTTCCCGTATGGAATGGTTTAACAGATGAAGATC
ATCCTACACAAGTACTTGCTGATTTTTTAACAGCTAAAGAAGTATTGAAAAAACCATATAATGAAATTAACTTCACATAT
GTTGGAGATGGACGTAATAACGTTGCAAATGCCTTAATGCAAGGAGCAGCAATCATGGGCATGACTTTCCATCTTGTATG
TCCTAAAGAACTAAATCCAACTGATGAATTATTAAATCGGTGTAATGATATAGCTGACAAAAACGGTGGTGAAATCCTTA
TAACTGATGATATTGATGAAGGTGTCAAAGGATCTGATGTAATTTACACAGATGTTTGGGTATCTATGGGTGAACCTGAT
GAAGTTTGGGAAAAACGTATCAAATTATTAGAACCATACCGTGTAACTAAAGAATTAATGAAAAAAACAGGTAATCCACA
TACAATTTTTGAACATTGCTTACCATCATTCCATGATACTGAAACAATAATCGGTAAACAAATTCAAGAAAAATATGGTC
TAACTGAAATGGAAGTAACAAATGAAGTATTTGAAAGTGAACAATCAGTTGTTTTCCAAGAAGCAGAAAATAGAGCACAT
ACAATAAAAGCAGTCATGGTAGCAACATTAGGAGAGTAA

Upstream 100 bases:

>100_bases
AGAGAATTAAAAATAGAAAATATTATATATCAAGATAAAAGAAATTGGATTATAAAAAAATAACATGAATAAAATATTAA
TTGAAAAGGAGATATTCAAA

Downstream 100 bases:

>100_bases
ATGAAAGGAGGATATAAATATGTCTAAAATCGTCGTAGCTTTGGGTGGTAACGCTTTAGGACAATCGCCTAAAGAACAAT
TAGATTTATTAAAAAGTACA

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase 1

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPT
GSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPD
EVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAH
TIKAVMVATLGE

Sequences:

>Translated_332_residues
MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPT
GSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPD
EVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAH
TIKAVMVATLGE
>Mature_332_residues
MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTRCAFETAAYDQGAHVTYLGPT
GSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTY
VGDGRNNVANALMQGAAIMGMTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPD
EVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVTNEVFESEQSVVFQEAENRAH
TIKAVMVATLGE

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=328, Percent_Identity=37.8048780487805, Blast_Score=203, Evalue=1e-52,
Organism=Escherichia coli, GI1790703, Length=332, Percent_Identity=58.1325301204819, Blast_Score=408, Evalue=1e-115,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=58.0838323353293, Blast_Score=405, Evalue=1e-114,
Organism=Escherichia coli, GI48994908, Length=372, Percent_Identity=22.5806451612903, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI2367364, Length=232, Percent_Identity=28.0172413793103, Blast_Score=71, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6322373, Length=328, Percent_Identity=28.3536585365854, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6322331, Length=190, Percent_Identity=30, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24642586, Length=230, Percent_Identity=26.9565217391304, Blast_Score=73, Evalue=3e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTCC1_STAES (Q8CU41)

Other databases:

- EMBL:   AE015929
- RefSeq:   NP_763658.1
- ProteinModelPortal:   Q8CU41
- SMR:   Q8CU41
- STRING:   Q8CU41
- EnsemblBacteria:   EBSTAT00000040533
- GeneID:   1056967
- GenomeReviews:   AE015929_GR
- KEGG:   sep:SE0103
- eggNOG:   COG0078
- GeneTree:   EBGT00050000023906
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- PhylomeDB:   Q8CU41
- ProtClustDB:   PRK04284
- BioCyc:   SEPI176280:SE_0103-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 37513; Mature: 37513

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 107-107 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTR
CCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCC
CAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKY
EEEHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTYVGDGRNNVANALMQGAAIMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHCHHHHC
MTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPD
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCHH
EVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVT
HHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
NEVFESEQSVVFQEAENRAHTIKAVMVATLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNLRNRNFLTLLDFTQKEMEFLLNLSEDLKRAKYAGIEQQKMKGKNIALLFEKDSTRTR
CCCCCCCCCEEEEHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHCCCCEEEEEECCCCCCC
CAFETAAYDQGAHVTYLGPTGSQMGKKESTKDTARVLGGMYDGIEYRGFSQRVVEDLAKY
EEEHHHHCCCCCEEEEECCCCHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
SGVPVWNGLTDEDHPTQVLADFLTAKEVLKKPYNEINFTYVGDGRNNVANALMQGAAIMG
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCEEEECCCCHHHHHHHHHCHHHHC
MTFHLVCPKELNPTDELLNRCNDIADKNGGEILITDDIDEGVKGSDVIYTDVWVSMGEPD
EEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCHH
EVWEKRIKLLEPYRVTKELMKKTGNPHTIFEHCLPSFHDTETIIGKQIQEKYGLTEMEVT
HHHHHHHHHHCHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHHH
NEVFESEQSVVFQEAENRAHTIKAVMVATLGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922