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The map label for this gene is dnaX [C]

Identifier: 160903372

GI number: 160903372

Start: 2153503

End: 2154543

Strand: Direct

Name: dnaX [C]

Synonym: Pmob_1943

Alternate gene names: 160903372

Gene position: 2153503-2154543 (Clockwise)

Preceding gene: 160903371

Following gene: 160903373

Centisome position: 99.26

GC content: 27.28

Gene sequence:

>1041_bases
TTGAGTGATTCATTTTTTTATGATACAATAGAAAAAATTCAATCGAAAATAGAAGTAATTAAGGCAAGTTCCATGTGTTT
TGTTGCAGAAGATAATTTTATTTTAAATAATTTTAGAGATGAATTATTAAATTCAGTTTCAGCTTTCAATCAGTTAGATA
CATTAATTATTAAACCTTCTGGTGGAAATATTAAGATAGAGCAAGTTAGGGATGTGGAAGAGTTTTTAATGTATAAACCT
AACTATGCAAAGGTGAAGGTTGTTTTTTTTGAGGATATTGATAGATTGAACATAGAAGCAGCAAATGCCGCACTCAAATT
AATCGAAGAGCCTCCAGAATATGCTTTAGTATTTGGAACTACTACGAGATGGAATTATTTGTTACCAACCATAAAAAGTC
GTTTTATAAGGTATGATCTTTCTATCCCTTCACTTTTGGTTGAAAATATTAATCGCAAATATGGTGAAATAGCGATTTAT
TTCAATTTTTTCCGACATTATGATTTGGGGGTTTTATTGTTTTTGAACGATGAAAGTACCGATGTTTCTAAAGTTAAAGA
TGAGGTCAAGAGGGTGCTTAACTTTTCATCCAACAATATCGATGAATTGATAAGCAATTTTAAATTATCTCTTGATTTGC
CATACAACAAACTGAAGTTTTCTCTTTCATATTATAAATTGTGGAATATATTAATAAATTCAGACGATAAAGAGTTCTTT
TATCTAATAAAAAATATTTCTCAAATAAAAAATGATGTTGAAAATTTATCTTTTTTAAGTAATATATCTTCTTTAGGTGC
TATTTTGTTGAGAGATGCGATGGTATTTTTGAATTCTTCCAAATGGAAATTTTTTTGGAACAACTCGTTAGTTTATTTCT
TTGGCTTAAATGAACATATTGTTAATTTAGAGCAAATTAAAGATAATATATCATATTTGAATAGGGTATCTACGATGAGA
TTGGCTAACTTTAACTTTGAAATGGAAATATTTACCCATTTTTTTAGAATTAAAAGATGCTTTATTAGAACCATTAAATA
A

Upstream 100 bases:

>100_bases
AAAGAGAGAAAAATACTTTAGTGTACGCGTCCAAAATTGATGAAATAAACGGATTGATTTTAGATTTGTACAAATAAAAG
TTGTATAGAAGGTGTTTTTT

Downstream 100 bases:

>100_bases
TAATACAATGGGAGTGAAATCTTTTGATAGATCTTGAAGCTGAAGTTTATGGGGTAGAGTTGGAAAAGTTAGGTCCCATA
TATTACTATAATTATATAGG

Product: DNA polymerase III gamma/tau subunits-like protein

Products: diphosphate; DNAn+1

Alternate protein names: DNA Polymerase III Gamma Subunit-Related Protein; DNA Polymerase III Delta Prime Subunit; DNA Polymerase III Delta Subunit

Number of amino acids: Translated: 346; Mature: 345

Protein sequence:

>346_residues
MSDSFFYDTIEKIQSKIEVIKASSMCFVAEDNFILNNFRDELLNSVSAFNQLDTLIIKPSGGNIKIEQVRDVEEFLMYKP
NYAKVKVVFFEDIDRLNIEAANAALKLIEEPPEYALVFGTTTRWNYLLPTIKSRFIRYDLSIPSLLVENINRKYGEIAIY
FNFFRHYDLGVLLFLNDESTDVSKVKDEVKRVLNFSSNNIDELISNFKLSLDLPYNKLKFSLSYYKLWNILINSDDKEFF
YLIKNISQIKNDVENLSFLSNISSLGAILLRDAMVFLNSSKWKFFWNNSLVYFFGLNEHIVNLEQIKDNISYLNRVSTMR
LANFNFEMEIFTHFFRIKRCFIRTIK

Sequences:

>Translated_346_residues
MSDSFFYDTIEKIQSKIEVIKASSMCFVAEDNFILNNFRDELLNSVSAFNQLDTLIIKPSGGNIKIEQVRDVEEFLMYKP
NYAKVKVVFFEDIDRLNIEAANAALKLIEEPPEYALVFGTTTRWNYLLPTIKSRFIRYDLSIPSLLVENINRKYGEIAIY
FNFFRHYDLGVLLFLNDESTDVSKVKDEVKRVLNFSSNNIDELISNFKLSLDLPYNKLKFSLSYYKLWNILINSDDKEFF
YLIKNISQIKNDVENLSFLSNISSLGAILLRDAMVFLNSSKWKFFWNNSLVYFFGLNEHIVNLEQIKDNISYLNRVSTMR
LANFNFEMEIFTHFFRIKRCFIRTIK
>Mature_345_residues
SDSFFYDTIEKIQSKIEVIKASSMCFVAEDNFILNNFRDELLNSVSAFNQLDTLIIKPSGGNIKIEQVRDVEEFLMYKPN
YAKVKVVFFEDIDRLNIEAANAALKLIEEPPEYALVFGTTTRWNYLLPTIKSRFIRYDLSIPSLLVENINRKYGEIAIYF
NFFRHYDLGVLLFLNDESTDVSKVKDEVKRVLNFSSNNIDELISNFKLSLDLPYNKLKFSLSYYKLWNILINSDDKEFFY
LIKNISQIKNDVENLSFLSNISSLGAILLRDAMVFLNSSKWKFFWNNSLVYFFGLNEHIVNLEQIKDNISYLNRVSTMRL
ANFNFEMEIFTHFFRIKRCFIRTIK

Specific function: DNA Polymerase III Is A Complex, Multichain Enzyme Responsible For Most Of The Replicative Synthesis In Bacteria. This DNA Polymerase Also Exhibits 3' To 5' Exonuclease Activity. The Tau Chain Serves As A Scaffold To Help In The Dimerizaton Of The Core Co

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.7

Molecular weight: Translated: 40775; Mature: 40644

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: PS00012 PHOSPHOPANTETHEINE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSFFYDTIEKIQSKIEVIKASSMCFVAEDNFILNNFRDELLNSVSAFNQLDTLIIKPS
CCCCHHHHHHHHHHHHHHHHEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
GGNIKIEQVRDVEEFLMYKPNYAKVKVVFFEDIDRLNIEAANAALKLIEEPPEYALVFGT
CCCEEEHHHHCHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEC
TTRWNYLLPTIKSRFIRYDLSIPSLLVENINRKYGEIAIYFNFFRHYDLGVLLFLNDEST
CCCCCCHHHHHHHHHEEEECCHHHHHHHHHCCCCCCEEEEEEEHHHCCCEEEEEECCCCC
DVSKVKDEVKRVLNFSSNNIDELISNFKLSLDLPYNKLKFSLSYYKLWNILINSDDKEFF
CHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCHHHEEEEHHHEEEEEEECCCCHHHH
YLIKNISQIKNDVENLSFLSNISSLGAILLRDAMVFLNSSKWKFFWNNSLVYFFGLNEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEECCHHH
VNLEQIKDNISYLNRVSTMRLANFNFEMEIFTHFFRIKRCFIRTIK
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSFFYDTIEKIQSKIEVIKASSMCFVAEDNFILNNFRDELLNSVSAFNQLDTLIIKPS
CCCHHHHHHHHHHHHHHHHEECCEEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEECC
GGNIKIEQVRDVEEFLMYKPNYAKVKVVFFEDIDRLNIEAANAALKLIEEPPEYALVFGT
CCCEEEHHHHCHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEEC
TTRWNYLLPTIKSRFIRYDLSIPSLLVENINRKYGEIAIYFNFFRHYDLGVLLFLNDEST
CCCCCCHHHHHHHHHEEEECCHHHHHHHHHCCCCCCEEEEEEEHHHCCCEEEEEECCCCC
DVSKVKDEVKRVLNFSSNNIDELISNFKLSLDLPYNKLKFSLSYYKLWNILINSDDKEFF
CHHHHHHHHHHHHCCCCCCHHHHHHCCEEEEECCCHHHEEEEHHHEEEEEEECCCCHHHH
YLIKNISQIKNDVENLSFLSNISSLGAILLRDAMVFLNSSKWKFFWNNSLVYFFGLNEHI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEECCCEEEEEECCHHH
VNLEQIKDNISYLNRVSTMRLANFNFEMEIFTHFFRIKRCFIRTIK
HHHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: deoxynucleoside triphosphate; DNAn

Specific reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA