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The map label for this gene is nadE2 [H]

Identifier: 160903300

GI number: 160903300

Start: 2061126

End: 2062853

Strand: Direct

Name: nadE2 [H]

Synonym: Pmob_1869

Alternate gene names: 160903300

Gene position: 2061126-2062853 (Clockwise)

Preceding gene: 160903299

Following gene: 160903301

Centisome position: 95.0

GC content: 33.97

Gene sequence:

>1728_bases
ATGAAAATAAGAATATCTTTGGCTCAAATGAATTCTACAGTTGGCGACTATCCAGGAAATGTTGAAAAGATAAAAGATTT
CATCTCTAAGGCAGACGAAAAAGGTGCCGATATAATATTATTTCCGGAATTATCTCTGAATGGATACCCACCTGAAGATT
TAATCTTAAAAACTCAATTTCTCAAGGATTCTTTGAAAAGTATAGAAGAAATACAAGATTTTAGTAAATCTAAAGATGTT
GTAATAGTTTTAGGTGCTGTTGACTGGGATGTTGAATCCTACAACTCTGCTTTTGTTATATACAAAGGCGAAATCTATGG
TAGTTATAAAAAGATGTTCTTACCAAACTACTCTGTTTTTGACGAAAAAAGGTATTTTACTGCCGGTAGAGCACCTTTTT
TAATGGAAATGGAAAGGATCAAAATTGGAATAACAATTTGTGAAGATCTGTGGGTTCCCAATGGACCGGCTGTTTCTTTA
GCCCAAAACGGAGCCAATTTGATTTTAAACCTTTCTTCATCTCCTTTTTATAAGGGGAGAAATAAAGTAAGGTTTGAAAT
GCTTAAAACTAGAGCATCAGAGTTATCGAGTTGGATCGCTTATTGCAATATTATAGGTGGTCAAGATGAGTTAGTTTTCG
ATGGTGGGAGTGTAGTTATTAACCCATACGGAGAAATAGAATTAAGTGCTCCTTCTTTTGAAGAAGGGTTGTATTTTATA
GATATAGATCCCCTAGAACCTACTAGAGCAAATTTAAGGGAAGGAAAAAGGAAACATTACAATCAAAGTGCCTACTATGA
AAGCGTAAATACAATCAAAATAGAAAAAAAGATTAGAGAAAAAACTCCTATAAAAGCTGTCAAAGTTGACTCTTTTGATA
TATACGAGCAATTGTACCTTGCTGTAAAAACTGGGATAAAAGATTATGTATTGAAAAATGGTTTCCAAAAAGTTGTTCTA
GGACTAAGTGGAGGAATAGATTCTTCGCTCACAGCCGCTATTGCAGCGGATGCTATTGGCCCTGAAAATGTTGTGGGATT
ACTAATGCCTTCTCAATACTCATCTAAAGGCAGTATCGACGATTCGATAGAGTTATCAAAAAATTTGGGAATAAATTATA
AAATAATACCAATAAATGACATATACGAAAAGTATATTGAAAATTTAAAGGAAAGCTTTAAAAACACCGATGAAGACAAA
ACTGAAGAAAACATTCAAGCAAGGATAAGGGGAAACTTAGTAATGGCATTTTCGAACAAATTCGGATATTTAGCCTTAGC
TTGCGGCAACAAAAGTGAAGCAGCTACAGGATACGCTACATTGTATGGAGACATGGCAGGAGGATTCTCTCCCATTAAAG
ACTTATACAAAACAGACTTATATAAAGTTGCCAGAAAATACAATGAACTGCATGGAAAGGAAATTATTATAAAATCGATA
TTAGAAAAAGCCCCTTCAGCGGAACTTCGACCAAATCAAAAAGATGAGGATATTTTGCCGCCATATGCTTTACTCGATGA
GATTTTATTCAAGTATATAGACAGAGAAATGTCATATGATGAATTACTACAGGAAGGATACGATGAAGGGTTATTGAAAA
ATGTAATAAACATGGTCAACAAGAACGAATACAAAAGAAGGCAATCTGCTCCTGGTATAAAACTAACTGAAAGAAGTTTT
GGAAAAGACAGAAGAATGCCAATTACAAATAAATATATCCCTTGGTAG

Upstream 100 bases:

>100_bases
ATTATGGCAAGAATGGTAAAATATATTGGTGTTTCTTTTTTCAATATCCGAAAAAGGAGGTCCATGCTTTTAAAAAATAA
ACCGAAAGCATAAATTAGCT

Downstream 100 bases:

>100_bases
TTAATGTCAAATGCACAGGGTTAGAGCGCTAAATAAGGATCTTTTTCCTTATGGGTGGGCTGCGAGGTCAAGAGATCTTC
GATCTTTTCCTTGTGGGGTG

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MKIRISLAQMNSTVGDYPGNVEKIKDFISKADEKGADIILFPELSLNGYPPEDLILKTQFLKDSLKSIEEIQDFSKSKDV
VIVLGAVDWDVESYNSAFVIYKGEIYGSYKKMFLPNYSVFDEKRYFTAGRAPFLMEMERIKIGITICEDLWVPNGPAVSL
AQNGANLILNLSSSPFYKGRNKVRFEMLKTRASELSSWIAYCNIIGGQDELVFDGGSVVINPYGEIELSAPSFEEGLYFI
DIDPLEPTRANLREGKRKHYNQSAYYESVNTIKIEKKIREKTPIKAVKVDSFDIYEQLYLAVKTGIKDYVLKNGFQKVVL
GLSGGIDSSLTAAIAADAIGPENVVGLLMPSQYSSKGSIDDSIELSKNLGINYKIIPINDIYEKYIENLKESFKNTDEDK
TEENIQARIRGNLVMAFSNKFGYLALACGNKSEAATGYATLYGDMAGGFSPIKDLYKTDLYKVARKYNELHGKEIIIKSI
LEKAPSAELRPNQKDEDILPPYALLDEILFKYIDREMSYDELLQEGYDEGLLKNVINMVNKNEYKRRQSAPGIKLTERSF
GKDRRMPITNKYIPW

Sequences:

>Translated_575_residues
MKIRISLAQMNSTVGDYPGNVEKIKDFISKADEKGADIILFPELSLNGYPPEDLILKTQFLKDSLKSIEEIQDFSKSKDV
VIVLGAVDWDVESYNSAFVIYKGEIYGSYKKMFLPNYSVFDEKRYFTAGRAPFLMEMERIKIGITICEDLWVPNGPAVSL
AQNGANLILNLSSSPFYKGRNKVRFEMLKTRASELSSWIAYCNIIGGQDELVFDGGSVVINPYGEIELSAPSFEEGLYFI
DIDPLEPTRANLREGKRKHYNQSAYYESVNTIKIEKKIREKTPIKAVKVDSFDIYEQLYLAVKTGIKDYVLKNGFQKVVL
GLSGGIDSSLTAAIAADAIGPENVVGLLMPSQYSSKGSIDDSIELSKNLGINYKIIPINDIYEKYIENLKESFKNTDEDK
TEENIQARIRGNLVMAFSNKFGYLALACGNKSEAATGYATLYGDMAGGFSPIKDLYKTDLYKVARKYNELHGKEIIIKSI
LEKAPSAELRPNQKDEDILPPYALLDEILFKYIDREMSYDELLQEGYDEGLLKNVINMVNKNEYKRRQSAPGIKLTERSF
GKDRRMPITNKYIPW
>Mature_575_residues
MKIRISLAQMNSTVGDYPGNVEKIKDFISKADEKGADIILFPELSLNGYPPEDLILKTQFLKDSLKSIEEIQDFSKSKDV
VIVLGAVDWDVESYNSAFVIYKGEIYGSYKKMFLPNYSVFDEKRYFTAGRAPFLMEMERIKIGITICEDLWVPNGPAVSL
AQNGANLILNLSSSPFYKGRNKVRFEMLKTRASELSSWIAYCNIIGGQDELVFDGGSVVINPYGEIELSAPSFEEGLYFI
DIDPLEPTRANLREGKRKHYNQSAYYESVNTIKIEKKIREKTPIKAVKVDSFDIYEQLYLAVKTGIKDYVLKNGFQKVVL
GLSGGIDSSLTAAIAADAIGPENVVGLLMPSQYSSKGSIDDSIELSKNLGINYKIIPINDIYEKYIENLKESFKNTDEDK
TEENIQARIRGNLVMAFSNKFGYLALACGNKSEAATGYATLYGDMAGGFSPIKDLYKTDLYKVARKYNELHGKEIIIKSI
LEKAPSAELRPNQKDEDILPPYALLDEILFKYIDREMSYDELLQEGYDEGLLKNVINMVNKNEYKRRQSAPGIKLTERSF
GKDRRMPITNKYIPW

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17538756, Length=580, Percent_Identity=21.8965517241379, Blast_Score=100, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6321865, Length=628, Percent_Identity=22.4522292993631, Blast_Score=100, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 64967; Mature: 64967

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRISLAQMNSTVGDYPGNVEKIKDFISKADEKGADIILFPELSLNGYPPEDLILKTQF
CEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHH
LKDSLKSIEEIQDFSKSKDVVIVLGAVDWDVESYNSAFVIYKGEIYGSYKKMFLPNYSVF
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEEECCEECCHHHHCCCCCCCC
DEKRYFTAGRAPFLMEMERIKIGITICEDLWVPNGPAVSLAQNGANLILNLSSSPFYKGR
CCCHHEECCCCCHHEEEEHEEEEEEEEHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCC
NKVRFEMLKTRASELSSWIAYCNIIGGQDELVFDGGSVVINPYGEIELSAPSFEEGLYFI
CHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCEEECCCCCEEECCCCHHCCEEEE
DIDPLEPTRANLREGKRKHYNQSAYYESVNTIKIEKKIREKTPIKAVKVDSFDIYEQLYL
ECCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEEEHHHHHHCCCCEEEEECCHHHHHHHHH
AVKTGIKDYVLKNGFQKVVLGLSGGIDSSLTAAIAADAIGPENVVGLLMPSQYSSKGSID
HHHHCHHHHHHHCCHHHHHHECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
DSIELSKNLGINYKIIPINDIYEKYIENLKESFKNTDEDKTEENIQARIRGNLVMAFSNK
CCHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCEEEEEECC
FGYLALACGNKSEAATGYATLYGDMAGGFSPIKDLYKTDLYKVARKYNELHGKEIIIKSI
CCEEEEEECCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LEKAPSAELRPNQKDEDILPPYALLDEILFKYIDREMSYDELLQEGYDEGLLKNVINMVN
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHC
KNEYKRRQSAPGIKLTERSFGKDRRMPITNKYIPW
CHHHHHHHCCCCCEEEHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKIRISLAQMNSTVGDYPGNVEKIKDFISKADEKGADIILFPELSLNGYPPEDLILKTQF
CEEEEEHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHH
LKDSLKSIEEIQDFSKSKDVVIVLGAVDWDVESYNSAFVIYKGEIYGSYKKMFLPNYSVF
HHHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCEEEEEECCEECCHHHHCCCCCCCC
DEKRYFTAGRAPFLMEMERIKIGITICEDLWVPNGPAVSLAQNGANLILNLSSSPFYKGR
CCCHHEECCCCCHHEEEEHEEEEEEEEHHCCCCCCCEEEEECCCCEEEEECCCCCCCCCC
NKVRFEMLKTRASELSSWIAYCNIIGGQDELVFDGGSVVINPYGEIELSAPSFEEGLYFI
CHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEECCCEEECCCCCEEECCCCHHCCEEEE
DIDPLEPTRANLREGKRKHYNQSAYYESVNTIKIEKKIREKTPIKAVKVDSFDIYEQLYL
ECCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEEEHHHHHHCCCCEEEEECCHHHHHHHHH
AVKTGIKDYVLKNGFQKVVLGLSGGIDSSLTAAIAADAIGPENVVGLLMPSQYSSKGSID
HHHHCHHHHHHHCCHHHHHHECCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCC
DSIELSKNLGINYKIIPINDIYEKYIENLKESFKNTDEDKTEENIQARIRGNLVMAFSNK
CCHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCHHHHHHHHEECCCEEEEEECC
FGYLALACGNKSEAATGYATLYGDMAGGFSPIKDLYKTDLYKVARKYNELHGKEIIIKSI
CCEEEEEECCCCCCCCCHHEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LEKAPSAELRPNQKDEDILPPYALLDEILFKYIDREMSYDELLQEGYDEGLLKNVINMVN
HHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHC
KNEYKRRQSAPGIKLTERSFGKDRRMPITNKYIPW
CHHHHHHHCCCCCEEEHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]