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The map label for this gene is truA

Identifier: 160903290

GI number: 160903290

Start: 2048616

End: 2049353

Strand: Direct

Name: truA

Synonym: Pmob_1859

Alternate gene names: 160903290

Gene position: 2048616-2049353 (Clockwise)

Preceding gene: 160903289

Following gene: 160903291

Centisome position: 94.43

GC content: 33.2

Gene sequence:

>738_bases
GTGAAATGGGTTGCAGCAACGGTAATGTATGATGGTTCAAACTTTTATGGTTATCAAAGTCAGCCAACATTTCGTACCGT
TCAAGATGAGTTTGAAAAAGCCTTAAAGGTTATTTTTAAAAAAGAAATTCCTTCTTATGCTTGTGGAAGAACTGATACAG
GAGTACATGCGGTAGGCCAAGTTATTTCCTTTAAAGTAGAAAATGAAAATATGACTGAAAAAAATATCAAAGATGCTTTG
AATGCTATTTTACCAGAAGATGTGTATGTAAAAGAAGTCAAAGAAGTAAAAGAAGGGTTTAATCCAAGGGCAGAGGCAAA
AAAAAGAATATATCATTATTTTATTTATATTAGTGAAGATCCAAATATATTTTTAAGAGACAGAGTATGGTGGATCCCTT
TTGCATTGAATTTAGAAAAGATGAGACAGGCAGCAAAATATTTTGAAGGGGAGCATGATTTTACCAGTTTTAAAACCGGT
AACGATGAGAGAAATCCCATAAGGACCATTTATAGGGTAAGAATAATAGAGTTAAGAAAAGATTTAATTTTGATAAGAGT
TGAAGGCAAGTCCTTTTTAAAAAGAATGGTTAGAAATATCGTTGGTTCATTGGTAAAGGTGGGTACAGATGTATGGGAAG
TGGAAAAAATACAGGAAATTTTGGAAGCAAAAAAAAGAGCTTTAGCACCTGCCTCAGCTCCTGCGCAAGGGTTGTATTTT
TACTCAGCTTTATTTTAA

Upstream 100 bases:

>100_bases
ATAAAAGAAATATAGATCTATTAAAAAGATTTGAATCGGAAATCGCATTCAAAATTTTAGAAGCTCAACTATTACAGAAG
ACATATTAGGAGGAGTATTC

Downstream 100 bases:

>100_bases
TAAATGGTATAATCTTAGCCAAAGTGGAGATTGGTTTAATTTATAATGGACCTTCTAACTATTTCGACGAGGTCTTGTTG
AATCTCTACAAATACGCGGA

Product: tRNA pseudouridine synthase ACD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MKWVAATVMYDGSNFYGYQSQPTFRTVQDEFEKALKVIFKKEIPSYACGRTDTGVHAVGQVISFKVENENMTEKNIKDAL
NAILPEDVYVKEVKEVKEGFNPRAEAKKRIYHYFIYISEDPNIFLRDRVWWIPFALNLEKMRQAAKYFEGEHDFTSFKTG
NDERNPIRTIYRVRIIELRKDLILIRVEGKSFLKRMVRNIVGSLVKVGTDVWEVEKIQEILEAKKRALAPASAPAQGLYF
YSALF

Sequences:

>Translated_245_residues
MKWVAATVMYDGSNFYGYQSQPTFRTVQDEFEKALKVIFKKEIPSYACGRTDTGVHAVGQVISFKVENENMTEKNIKDAL
NAILPEDVYVKEVKEVKEGFNPRAEAKKRIYHYFIYISEDPNIFLRDRVWWIPFALNLEKMRQAAKYFEGEHDFTSFKTG
NDERNPIRTIYRVRIIELRKDLILIRVEGKSFLKRMVRNIVGSLVKVGTDVWEVEKIQEILEAKKRALAPASAPAQGLYF
YSALF
>Mature_245_residues
MKWVAATVMYDGSNFYGYQSQPTFRTVQDEFEKALKVIFKKEIPSYACGRTDTGVHAVGQVISFKVENENMTEKNIKDAL
NAILPEDVYVKEVKEVKEGFNPRAEAKKRIYHYFIYISEDPNIFLRDRVWWIPFALNLEKMRQAAKYFEGEHDFTSFKTG
NDERNPIRTIYRVRIIELRKDLILIRVEGKSFLKRMVRNIVGSLVKVGTDVWEVEKIQEILEAKKRALAPASAPAQGLYF
YSALF

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=265, Percent_Identity=26.7924528301887, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI70166645, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI70166634, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI70166599, Length=231, Percent_Identity=26.4069264069264, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI199559785, Length=271, Percent_Identity=24.7232472324723, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI1788657, Length=243, Percent_Identity=37.8600823045267, Blast_Score=148, Evalue=3e-37,
Organism=Caenorhabditis elegans, GI71985046, Length=273, Percent_Identity=26.3736263736264, Blast_Score=70, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6321112, Length=254, Percent_Identity=27.1653543307087, Blast_Score=72, Evalue=7e-14,
Organism=Drosophila melanogaster, GI281362146, Length=243, Percent_Identity=26.7489711934156, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648455, Length=243, Percent_Identity=26.7489711934156, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_PETMO (A9BGW7)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568871.1
- ProteinModelPortal:   A9BGW7
- SMR:   A9BGW7
- GeneID:   5756950
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1859
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK14586
- BioCyc:   PMOB403833:PMOB_1859-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 28525; Mature: 28525

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKWVAATVMYDGSNFYGYQSQPTFRTVQDEFEKALKVIFKKEIPSYACGRTDTGVHAVGQ
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VISFKVENENMTEKNIKDALNAILPEDVYVKEVKEVKEGFNPRAEAKKRIYHYFIYISED
HHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHEEEEEEEEECCC
PNIFLRDRVWWIPFALNLEKMRQAAKYFEGEHDFTSFKTGNDERNPIRTIYRVRIIELRK
CCEEEECEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DLILIRVEGKSFLKRMVRNIVGSLVKVGTDVWEVEKIQEILEAKKRALAPASAPAQGLYF
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSALF
HHHCC
>Mature Secondary Structure
MKWVAATVMYDGSNFYGYQSQPTFRTVQDEFEKALKVIFKKEIPSYACGRTDTGVHAVGQ
CCEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
VISFKVENENMTEKNIKDALNAILPEDVYVKEVKEVKEGFNPRAEAKKRIYHYFIYISED
HHEEEECCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHEEEEEEEEECCC
PNIFLRDRVWWIPFALNLEKMRQAAKYFEGEHDFTSFKTGNDERNPIRTIYRVRIIELRK
CCEEEECEEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCC
DLILIRVEGKSFLKRMVRNIVGSLVKVGTDVWEVEKIQEILEAKKRALAPASAPAQGLYF
CEEEEEECCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHH
YSALF
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA