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The map label for this gene is cysS

Identifier: 160903262

GI number: 160903262

Start: 2016131

End: 2017540

Strand: Direct

Name: cysS

Synonym: Pmob_1831

Alternate gene names: 160903262

Gene position: 2016131-2017540 (Clockwise)

Preceding gene: 160903261

Following gene: 160903263

Centisome position: 92.93

GC content: 34.54

Gene sequence:

>1410_bases
TTGAAAAGATTTCCTACAATTAAAATATACGATACTTTAAGTGGACAAGTAGTTGATTTAGTACCGGTGAAAGAAGGCGA
AATAAAGATTTATCTTTGTGGACCAACAGTCTACAACTTGTTACACATTGGCAATGCAAGACCTATTATAATATTTGATG
CCTTTAGACGTTTTTTGGAATATATTGGTTACAAAGTTACATTAGTACAGAACTTCACTGATATAGACGATAAGATCATT
GAACAAGCAAAAAAAGAGAACTTACCTTTCGAAGAGGTAGGGAAAAGATATATAATTGAATACTGGAGAGATATGACGGC
TCTTAAAGCTAGGGCTTTTAATTTCCACCCAAAAACTACCAACTATGTTGATGAAATTATTTCTTATATAAAAGAACTTG
AAGAAAAAGGCTATGCTTATAAGGCTGAAAATGGAGATGTTTACTTTGAAGTGGAAAAATTTTCAAGATATGGAGAGTTA
TCTCACAGAAAGGTCGAAGATCTGAAAGTAGGGGTTAGGGTAGAAGTCTCCGAATACAAAAAAAATCCTTTGGACTTTGC
CCTATGGAAGGCTTCAAAAGAAGGGGAACCATTTTGGGAAAGTCCATGGGGAAAAGGTCGCCCAGGGTGGCACATAGAAT
GTTCTGTTATGTCGTCTGAAATATTAGGCGACACCTTTGATATTCATGCGGGAGGTAACGATCTCATCTTTCCTCATCAC
GAGAACGAAAGAGCCCAAGCTATAGCAAAAAGTGGTAAGGATTTTGCAAAATACTGGATGCACAACGGAATGATTCGTAT
GGCACAAGATAAGATGTCAAAATCTTTGGGGAACGTCTGGTATTTAAGAGACCTTTTGAAAAAGTTTGATTCGGATGTCT
TAAAAATTTTTATACTCAGCAAGCATTATCGTATACCTATAGATGTAAGTGAAGAATTGCTTCGTAACCAAGAGGTATCA
GTTAATAGAGTTAAAGAATCTTTGAATGAGGCTGAAACTTTCTTTAACGGGAAAGTCCCTTGTCCTCAGAAAATGAATTA
TTTTAAAGAGCAAGAGGAGTATCTCATTAGTAGCCTTTCGAATGATTTTGATACACCATCAGTTGTGGCAAGAATTTTCG
AATTATCAAGAGATTTAAACAAAGCGTTAAATTCACGTGATGAAGAAACAATAAAAAATAATTATTATATAATAAGAAAT
ATTTACGGGAGTGTCCTGGGGGTTTTTGAAACTAACGAACAAATACAGAAGAACAATGTAGAATTAAATCAACTAATGGA
AATCATTCTAAACGTAAGATCTGCTCTTAGAGAAGAAAAGTTATACAATCTTTCAGACTATATACGGGATAACTTAAGCA
AAATTGGCATAGAAATAAAAGATACCCCTGAAGGAACAAAATGGTCTTAG

Upstream 100 bases:

>100_bases
AAATTTTAACGGATTCATTTCATGGACCCGATTTGATAAATACGATATTTTTATTAGGTAGGAATACTGTTTTGGAAAGA
CTTCAAAGGGTGATCGAGAT

Downstream 100 bases:

>100_bases
AACTGGAGGTGTTCTCAAATAGACGTTAATATAATAAACTCAGTACTCGCCTCCCTCAAAAACACCTTTAAAATAATCAC
AAAGATGGATATACAATTGC

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKRFPTIKIYDTLSGQVVDLVPVKEGEIKIYLCGPTVYNLLHIGNARPIIIFDAFRRFLEYIGYKVTLVQNFTDIDDKII
EQAKKENLPFEEVGKRYIIEYWRDMTALKARAFNFHPKTTNYVDEIISYIKELEEKGYAYKAENGDVYFEVEKFSRYGEL
SHRKVEDLKVGVRVEVSEYKKNPLDFALWKASKEGEPFWESPWGKGRPGWHIECSVMSSEILGDTFDIHAGGNDLIFPHH
ENERAQAIAKSGKDFAKYWMHNGMIRMAQDKMSKSLGNVWYLRDLLKKFDSDVLKIFILSKHYRIPIDVSEELLRNQEVS
VNRVKESLNEAETFFNGKVPCPQKMNYFKEQEEYLISSLSNDFDTPSVVARIFELSRDLNKALNSRDEETIKNNYYIIRN
IYGSVLGVFETNEQIQKNNVELNQLMEIILNVRSALREEKLYNLSDYIRDNLSKIGIEIKDTPEGTKWS

Sequences:

>Translated_469_residues
MKRFPTIKIYDTLSGQVVDLVPVKEGEIKIYLCGPTVYNLLHIGNARPIIIFDAFRRFLEYIGYKVTLVQNFTDIDDKII
EQAKKENLPFEEVGKRYIIEYWRDMTALKARAFNFHPKTTNYVDEIISYIKELEEKGYAYKAENGDVYFEVEKFSRYGEL
SHRKVEDLKVGVRVEVSEYKKNPLDFALWKASKEGEPFWESPWGKGRPGWHIECSVMSSEILGDTFDIHAGGNDLIFPHH
ENERAQAIAKSGKDFAKYWMHNGMIRMAQDKMSKSLGNVWYLRDLLKKFDSDVLKIFILSKHYRIPIDVSEELLRNQEVS
VNRVKESLNEAETFFNGKVPCPQKMNYFKEQEEYLISSLSNDFDTPSVVARIFELSRDLNKALNSRDEETIKNNYYIIRN
IYGSVLGVFETNEQIQKNNVELNQLMEIILNVRSALREEKLYNLSDYIRDNLSKIGIEIKDTPEGTKWS
>Mature_469_residues
MKRFPTIKIYDTLSGQVVDLVPVKEGEIKIYLCGPTVYNLLHIGNARPIIIFDAFRRFLEYIGYKVTLVQNFTDIDDKII
EQAKKENLPFEEVGKRYIIEYWRDMTALKARAFNFHPKTTNYVDEIISYIKELEEKGYAYKAENGDVYFEVEKFSRYGEL
SHRKVEDLKVGVRVEVSEYKKNPLDFALWKASKEGEPFWESPWGKGRPGWHIECSVMSSEILGDTFDIHAGGNDLIFPHH
ENERAQAIAKSGKDFAKYWMHNGMIRMAQDKMSKSLGNVWYLRDLLKKFDSDVLKIFILSKHYRIPIDVSEELLRNQEVS
VNRVKESLNEAETFFNGKVPCPQKMNYFKEQEEYLISSLSNDFDTPSVVARIFELSRDLNKALNSRDEETIKNNYYIIRN
IYGSVLGVFETNEQIQKNNVELNQLMEIILNVRSALREEKLYNLSDYIRDNLSKIGIEIKDTPEGTKWS

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI13375695, Length=505, Percent_Identity=30.8910891089109, Blast_Score=251, Evalue=1e-66,
Organism=Homo sapiens, GI303304987, Length=425, Percent_Identity=31.0588235294118, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI21269877, Length=425, Percent_Identity=31.0588235294118, Blast_Score=187, Evalue=2e-47,
Organism=Homo sapiens, GI10835051, Length=425, Percent_Identity=31.0588235294118, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI62240992, Length=425, Percent_Identity=31.0588235294118, Blast_Score=186, Evalue=3e-47,
Organism=Escherichia coli, GI1786737, Length=468, Percent_Identity=39.5299145299145, Blast_Score=360, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17509797, Length=380, Percent_Identity=32.8947368421053, Blast_Score=190, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI17509795, Length=380, Percent_Identity=32.8947368421053, Blast_Score=189, Evalue=2e-48,
Organism=Saccharomyces cerevisiae, GI6324082, Length=391, Percent_Identity=32.4808184143223, Blast_Score=181, Evalue=2e-46,
Organism=Drosophila melanogaster, GI19922218, Length=499, Percent_Identity=31.2625250501002, Blast_Score=248, Evalue=7e-66,
Organism=Drosophila melanogaster, GI19922364, Length=243, Percent_Identity=38.2716049382716, Blast_Score=175, Evalue=5e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYC_PETMO (A9BGT9)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568843.1
- ProteinModelPortal:   A9BGT9
- SMR:   A9BGT9
- GeneID:   5758047
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1831
- HOGENOM:   HBG327651
- OMA:   DFDALNM
- ProtClustDB:   PRK00260
- BioCyc:   PMOB403833:PMOB_1831-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00041_B
- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10890
- PRINTS:   PR00983
- SMART:   SM00840
- TIGRFAMs:   TIGR00435

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e; SSF47323 tRNAsyn_1a_bind

EC number: =6.1.1.16

Molecular weight: Translated: 54789; Mature: 54789

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 274-274

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFPTIKIYDTLSGQVVDLVPVKEGEIKIYLCGPTVYNLLHIGNARPIIIFDAFRRFLE
CCCCCEEEEEECCCCCEEEEEECCCCCEEEEEECHHHHHHHHCCCCCEEEEHHHHHHHHH
YIGYKVTLVQNFTDIDDKIIEQAKKENLPFEEVGKRYIIEYWRDMTALKARAFNFHPKTT
HHCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
NYVDEIISYIKELEEKGYAYKAENGDVYFEVEKFSRYGELSHRKVEDLKVGVRVEVSEYK
HHHHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHHHCCCHHHCCHHHHEECEEEEHHHHC
KNPLDFALWKASKEGEPFWESPWGKGRPGWHIECSVMSSEILGDTFDIHAGGNDLIFPHH
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEECCCCCEECCCC
ENERAQAIAKSGKDFAKYWMHNGMIRMAQDKMSKSLGNVWYLRDLLKKFDSDVLKIFILS
CCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHCC
KHYRIPIDVSEELLRNQEVSVNRVKESLNEAETFFNGKVPCPQKMNYFKEQEEYLISSLS
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
NDFDTPSVVARIFELSRDLNKALNSRDEETIKNNYYIIRNIYGSVLGVFETNEQIQKNNV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHCCC
ELNQLMEIILNVRSALREEKLYNLSDYIRDNLSKIGIEIKDTPEGTKWS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCC
>Mature Secondary Structure
MKRFPTIKIYDTLSGQVVDLVPVKEGEIKIYLCGPTVYNLLHIGNARPIIIFDAFRRFLE
CCCCCEEEEEECCCCCEEEEEECCCCCEEEEEECHHHHHHHHCCCCCEEEEHHHHHHHHH
YIGYKVTLVQNFTDIDDKIIEQAKKENLPFEEVGKRYIIEYWRDMTALKARAFNFHPKTT
HHCEEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
NYVDEIISYIKELEEKGYAYKAENGDVYFEVEKFSRYGELSHRKVEDLKVGVRVEVSEYK
HHHHHHHHHHHHHHHCCCEEEECCCCEEEEEHHHHHCCCHHHCCHHHHEECEEEEHHHHC
KNPLDFALWKASKEGEPFWESPWGKGRPGWHIECSVMSSEILGDTFDIHAGGNDLIFPHH
CCCCCEEEEECCCCCCCCCCCCCCCCCCCCEEEEEEEHHHHCCCCEEEECCCCCEECCCC
ENERAQAIAKSGKDFAKYWMHNGMIRMAQDKMSKSLGNVWYLRDLLKKFDSDVLKIFILS
CCHHHHHHHHCCHHHHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHCHHHHHHHHCC
KHYRIPIDVSEELLRNQEVSVNRVKESLNEAETFFNGKVPCPQKMNYFKEQEEYLISSLS
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHC
NDFDTPSVVARIFELSRDLNKALNSRDEETIKNNYYIIRNIYGSVLGVFETNEQIQKNNV
CCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEEEEEHHHHHHHHHCCCHHHHHCCC
ELNQLMEIILNVRSALREEKLYNLSDYIRDNLSKIGIEIKDTPEGTKWS
CHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA