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The map label for this gene is gltX

Identifier: 160903261

GI number: 160903261

Start: 2014668

End: 2016134

Strand: Direct

Name: gltX

Synonym: Pmob_1830

Alternate gene names: 160903261

Gene position: 2014668-2016134 (Clockwise)

Preceding gene: 160903260

Following gene: 160903262

Centisome position: 92.86

GC content: 33.54

Gene sequence:

>1467_bases
ATGATCTCAAATACTGTAAGAACAAGGTTTGCACCAAGTCCAACAGGATATCTCCACGTTGGTGGAGCTAGAACGGCACT
TTTTAATTATCTCTTTTCCAAAAAAAATGAAGGAAAATTTATCTTAAGAATCGAAGATACAGACATAGAACGTTCAACAA
AAGAATCTGAAGATCAACTTATAAACACTCTAAAATGGTTGAATTTGAATTGGGATGAAGGACCTATAATTGGCGGAGAT
TATGAACCTTATCGCCAAAGTGAAAGGTTAGAAATCTATCAGCGAAGAGCAAAGGAACTAATAGAAAAAGGTAAAGCATA
CGAGGCATATATCTCACCAGAAGAAATAGAAGAAGTAAAAAACCAGTTAATAAGCGAAGGTAAACCGCCACATTATACCT
ATGATTTGATTTCAAAGTACAACACAAAGGAAAGAATTGAAGAGTACAATTACAAAGGGTTAAAACCCGTTATTTTTTTG
AAGATGCCTCAGAAAGATTATGAACTCGATGATAAAATAAAGGGTAAAGTTATCTTTAAGAAAGGTTCTATTGGTGATTT
TATAATTTTAAGAAGTAATGGTATACCTACATATAATTTTGCTGTCGTTGTAGACGATATCGAAATGAAAATCACCCATG
TTATCAGGGGGGACGATCATTTACCAAATACTTTGCGACAAATGGCGATTTATGAGGCTTTTGAGGTGGAACCCCCAATA
TTTGCACATGTTTCAATGATCTTGGGACCAGATGGCAAAAAATTATCCAAAAGACATGGAGCTACCTCTGTCGAAGAGTT
TATGGTTCAAGGATATTTGCCTGAAGCTGTTGACAATTATCTGGCACTGCTTGGTTGGTCTCATCCTGAGGGTAAGGAAA
TATTGCCTCTCTCTGAAATAATTGATAATTTTACTTTGGATAGAGTCAATTCAAGCCCTGCAATATTCGATGAGAAAAAA
TTAAAATGGATGAATGGGCAGTATCTAAGAAATAAGCCTTTGGAAGAAATCTATCAACTTGCAGAACCTTTTGTGACTGG
ATCAAAACTTCTAAGCAAAGAACAATATGAAGTAAACAAAAAATGGGTTATTAAAGCTTTAGAAACTATTCTTTCTTCTG
TTGAAATATTGAGCGAAATACCTGAAAAAATGGAAGTTTTTTTAAAAGATGTTTATCCAGATACAAACGATCCTGAATTT
AAAGAATACTTTGATAAAAGAGGAGTAAAAGAAGCTATCAACTTGATTTATAAGTATTTCAATGAAGATGACAGATGGGA
TACACAAACTATAACAGAGAATTTAAAGAATGCTATGAACGAAGCCAATCCTCAAAAGAAACCATTTTACATGTCTTTAA
GAAAAATTTTAACGGATTCATTTCATGGACCCGATTTGATAAATACGATATTTTTATTAGGTAGGAATACTGTTTTGGAA
AGACTTCAAAGGGTGATCGAGATTTGA

Upstream 100 bases:

>100_bases
GTCCATCCTTAAAAGGGTGGGCAGCGGGGCGAAGGGGCGCTAAAACAAATTTTAGAGTTTCTAAAAACAATAAAATCAAA
AAATTTTGGAGGACTAAACA

Downstream 100 bases:

>100_bases
AAAGATTTCCTACAATTAAAATATACGATACTTTAAGTGGACAAGTAGTTGATTTAGTACCGGTGAAAGAAGGCGAAATA
AAGATTTATCTTTGTGGACC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase 2; GluRS 2

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MISNTVRTRFAPSPTGYLHVGGARTALFNYLFSKKNEGKFILRIEDTDIERSTKESEDQLINTLKWLNLNWDEGPIIGGD
YEPYRQSERLEIYQRRAKELIEKGKAYEAYISPEEIEEVKNQLISEGKPPHYTYDLISKYNTKERIEEYNYKGLKPVIFL
KMPQKDYELDDKIKGKVIFKKGSIGDFIILRSNGIPTYNFAVVVDDIEMKITHVIRGDDHLPNTLRQMAIYEAFEVEPPI
FAHVSMILGPDGKKLSKRHGATSVEEFMVQGYLPEAVDNYLALLGWSHPEGKEILPLSEIIDNFTLDRVNSSPAIFDEKK
LKWMNGQYLRNKPLEEIYQLAEPFVTGSKLLSKEQYEVNKKWVIKALETILSSVEILSEIPEKMEVFLKDVYPDTNDPEF
KEYFDKRGVKEAINLIYKYFNEDDRWDTQTITENLKNAMNEANPQKKPFYMSLRKILTDSFHGPDLINTIFLLGRNTVLE
RLQRVIEI

Sequences:

>Translated_488_residues
MISNTVRTRFAPSPTGYLHVGGARTALFNYLFSKKNEGKFILRIEDTDIERSTKESEDQLINTLKWLNLNWDEGPIIGGD
YEPYRQSERLEIYQRRAKELIEKGKAYEAYISPEEIEEVKNQLISEGKPPHYTYDLISKYNTKERIEEYNYKGLKPVIFL
KMPQKDYELDDKIKGKVIFKKGSIGDFIILRSNGIPTYNFAVVVDDIEMKITHVIRGDDHLPNTLRQMAIYEAFEVEPPI
FAHVSMILGPDGKKLSKRHGATSVEEFMVQGYLPEAVDNYLALLGWSHPEGKEILPLSEIIDNFTLDRVNSSPAIFDEKK
LKWMNGQYLRNKPLEEIYQLAEPFVTGSKLLSKEQYEVNKKWVIKALETILSSVEILSEIPEKMEVFLKDVYPDTNDPEF
KEYFDKRGVKEAINLIYKYFNEDDRWDTQTITENLKNAMNEANPQKKPFYMSLRKILTDSFHGPDLINTIFLLGRNTVLE
RLQRVIEI
>Mature_488_residues
MISNTVRTRFAPSPTGYLHVGGARTALFNYLFSKKNEGKFILRIEDTDIERSTKESEDQLINTLKWLNLNWDEGPIIGGD
YEPYRQSERLEIYQRRAKELIEKGKAYEAYISPEEIEEVKNQLISEGKPPHYTYDLISKYNTKERIEEYNYKGLKPVIFL
KMPQKDYELDDKIKGKVIFKKGSIGDFIILRSNGIPTYNFAVVVDDIEMKITHVIRGDDHLPNTLRQMAIYEAFEVEPPI
FAHVSMILGPDGKKLSKRHGATSVEEFMVQGYLPEAVDNYLALLGWSHPEGKEILPLSEIIDNFTLDRVNSSPAIFDEKK
LKWMNGQYLRNKPLEEIYQLAEPFVTGSKLLSKEQYEVNKKWVIKALETILSSVEILSEIPEKMEVFLKDVYPDTNDPEF
KEYFDKRGVKEAINLIYKYFNEDDRWDTQTITENLKNAMNEANPQKKPFYMSLRKILTDSFHGPDLINTIFLLGRNTVLE
RLQRVIEI

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=327, Percent_Identity=37.9204892966361, Blast_Score=238, Evalue=9e-63,
Organism=Escherichia coli, GI1788743, Length=484, Percent_Identity=34.9173553719008, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI226510925, Length=257, Percent_Identity=30.7392996108949, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1786895, Length=128, Percent_Identity=34.375, Blast_Score=75, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI71993394, Length=342, Percent_Identity=38.3040935672515, Blast_Score=216, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6324540, Length=496, Percent_Identity=30.8467741935484, Blast_Score=237, Evalue=3e-63,
Organism=Saccharomyces cerevisiae, GI6321192, Length=213, Percent_Identity=28.6384976525822, Blast_Score=74, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=115, Percent_Identity=31.304347826087, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665321, Length=499, Percent_Identity=31.6633266533066, Blast_Score=239, Evalue=3e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE2_PETMO (A9BGT8)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568842.1
- ProteinModelPortal:   A9BGT8
- SMR:   A9BGT8
- GeneID:   5758046
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1830
- HOGENOM:   HBG628189
- OMA:   LEDTDQK
- ProtClustDB:   PRK01406
- BioCyc:   PMOB403833:PMOB_1830-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 56769; Mature: 56769

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 257-257

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISNTVRTRFAPSPTGYLHVGGARTALFNYLFSKKNEGKFILRIEDTDIERSTKESEDQL
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
INTLKWLNLNWDEGPIIGGDYEPYRQSERLEIYQRRAKELIEKGKAYEAYISPEEIEEVK
HHHHHHEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
NQLISEGKPPHYTYDLISKYNTKERIEEYNYKGLKPVIFLKMPQKDYELDDKIKGKVIFK
HHHHHCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEE
KGSIGDFIILRSNGIPTYNFAVVVDDIEMKITHVIRGDDHLPNTLRQMAIYEAFEVEPPI
ECCCCCEEEEECCCCCCEEEEEEEECCCEEEEHEECCCCCCHHHHHHHHHHHHHCCCCCH
FAHVSMILGPDGKKLSKRHGATSVEEFMVQGYLPEAVDNYLALLGWSHPEGKEILPLSEI
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
IDNFTLDRVNSSPAIFDEKKLKWMNGQYLRNKPLEEIYQLAEPFVTGSKLLSKEQYEVNK
HCCCCHHCCCCCCCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWVIKALETILSSVEILSEIPEKMEVFLKDVYPDTNDPEFKEYFDKRGVKEAINLIYKYF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
NEDDRWDTQTITENLKNAMNEANPQKKPFYMSLRKILTDSFHGPDLINTIFLLGRNTVLE
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
RLQRVIEI
HHHHHHCC
>Mature Secondary Structure
MISNTVRTRFAPSPTGYLHVGGARTALFNYLFSKKNEGKFILRIEDTDIERSTKESEDQL
CCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHH
INTLKWLNLNWDEGPIIGGDYEPYRQSERLEIYQRRAKELIEKGKAYEAYISPEEIEEVK
HHHHHHEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHH
NQLISEGKPPHYTYDLISKYNTKERIEEYNYKGLKPVIFLKMPQKDYELDDKIKGKVIFK
HHHHHCCCCCCCHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCEEEEE
KGSIGDFIILRSNGIPTYNFAVVVDDIEMKITHVIRGDDHLPNTLRQMAIYEAFEVEPPI
ECCCCCEEEEECCCCCCEEEEEEEECCCEEEEHEECCCCCCHHHHHHHHHHHHHCCCCCH
FAHVSMILGPDGKKLSKRHGATSVEEFMVQGYLPEAVDNYLALLGWSHPEGKEILPLSEI
HHHHHHHCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHH
IDNFTLDRVNSSPAIFDEKKLKWMNGQYLRNKPLEEIYQLAEPFVTGSKLLSKEQYEVNK
HCCCCHHCCCCCCCCCCHHHHHHCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
KWVIKALETILSSVEILSEIPEKMEVFLKDVYPDTNDPEFKEYFDKRGVKEAINLIYKYF
HHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
NEDDRWDTQTITENLKNAMNEANPQKKPFYMSLRKILTDSFHGPDLINTIFLLGRNTVLE
CCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHH
RLQRVIEI
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA