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The map label for this gene is grpE [H]

Identifier: 160903255

GI number: 160903255

Start: 2009101

End: 2009805

Strand: Direct

Name: grpE [H]

Synonym: Pmob_1824

Alternate gene names: 160903255

Gene position: 2009101-2009805 (Clockwise)

Preceding gene: 160903254

Following gene: 160903256

Centisome position: 92.6

GC content: 31.77

Gene sequence:

>705_bases
ATGGAAGAAAGAAAAAAAACTGAAGAAATAACAGAACAAGAGGAACAAAAAGAAATAAAAGAAAAAGAATTAAGTCAGGA
AGATATCGAATCGTTGAAAGAGAAATTAGAAGAATTAGAAAAAGAAAATAATGAGTTAAAACACGAGTTGAAAAATCTAG
AAAAAGAAAGAGATGAATTTAAAGAGTATTCTATATATCTTAAAACTAAATTTGAAGATTACAAAAATTTAGTGGAGAAA
GAAAAAAAGCAGATAAAACTGAACACAACAAAAATGATAATAGAAAAATTACTTGTCCCATTTGAAAAGTTGAATCTTTC
TTTGAATTACAAAGATGAACCCGAATTTGTTTCTGCTGTTGAGATGGTTTACAAAGATATGTTGAAAGTCTTTGACTCCT
TGAAAATGAATTTTATAGTTCCACAAAAAGGAGATCAATTTGATCCATTCGAACATGATGTTGTTGACAAGTTTGAAACA
AAAGAAGTGAGTGAATACTGTATATACGATGTCCAATCCATCGGGTACAAATTGGAAGGTGAAGTAATAAAACCAGCAAG
GGTTATAGTGGCTGTTAAACCGAAGGAGAACGCCCCGAACGGGGTCAAGGAAAAAGTTGAAGACAAGAAAAACCCTTGTG
ACGAAAATGTTGATTCTGACGAACAAACTGCGGAAGGGATCGACTCAAAAGAAGGTGACAAATAA

Upstream 100 bases:

>100_bases
TTGAATTCGGGGTTTTTAAGGGGCTTGCCCCTTAACGGTTGGTGAAAGGGTGCAAACTTAAAAATAGTAATGATCAGTTT
TTAGGAGGTGTTTTTTTGAA

Downstream 100 bases:

>100_bases
TTATGGCGGAAAGAAAAGATTATTACAAAATCTTGGGAGTAGATAGAAACGCATCACAGGAAGAGATAAAAAAAGCCTAT
AGACAAAAAGTCAAAGAATG

Product: GrpE protein

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MEERKKTEEITEQEEQKEIKEKELSQEDIESLKEKLEELEKENNELKHELKNLEKERDEFKEYSIYLKTKFEDYKNLVEK
EKKQIKLNTTKMIIEKLLVPFEKLNLSLNYKDEPEFVSAVEMVYKDMLKVFDSLKMNFIVPQKGDQFDPFEHDVVDKFET
KEVSEYCIYDVQSIGYKLEGEVIKPARVIVAVKPKENAPNGVKEKVEDKKNPCDENVDSDEQTAEGIDSKEGDK

Sequences:

>Translated_234_residues
MEERKKTEEITEQEEQKEIKEKELSQEDIESLKEKLEELEKENNELKHELKNLEKERDEFKEYSIYLKTKFEDYKNLVEK
EKKQIKLNTTKMIIEKLLVPFEKLNLSLNYKDEPEFVSAVEMVYKDMLKVFDSLKMNFIVPQKGDQFDPFEHDVVDKFET
KEVSEYCIYDVQSIGYKLEGEVIKPARVIVAVKPKENAPNGVKEKVEDKKNPCDENVDSDEQTAEGIDSKEGDK
>Mature_234_residues
MEERKKTEEITEQEEQKEIKEKELSQEDIESLKEKLEELEKENNELKHELKNLEKERDEFKEYSIYLKTKFEDYKNLVEK
EKKQIKLNTTKMIIEKLLVPFEKLNLSLNYKDEPEFVSAVEMVYKDMLKVFDSLKMNFIVPQKGDQFDPFEHDVVDKFET
KEVSEYCIYDVQSIGYKLEGEVIKPARVIVAVKPKENAPNGVKEKVEDKKNPCDENVDSDEQTAEGIDSKEGDK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

None

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 27629; Mature: 27629

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEERKKTEEITEQEEQKEIKEKELSQEDIESLKEKLEELEKENNELKHELKNLEKERDEF
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYSIYLKTKFEDYKNLVEKEKKQIKLNTTKMIIEKLLVPFEKLNLSLNYKDEPEFVSAV
HHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEECCCCCCHHHHHHH
EMVYKDMLKVFDSLKMNFIVPQKGDQFDPFEHDVVDKFETKEVSEYCIYDVQSIGYKLEG
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
EVIKPARVIVAVKPKENAPNGVKEKVEDKKNPCDENVDSDEQTAEGIDSKEGDK
CEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MEERKKTEEITEQEEQKEIKEKELSQEDIESLKEKLEELEKENNELKHELKNLEKERDEF
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KEYSIYLKTKFEDYKNLVEKEKKQIKLNTTKMIIEKLLVPFEKLNLSLNYKDEPEFVSAV
HHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCEECCCCCCHHHHHHH
EMVYKDMLKVFDSLKMNFIVPQKGDQFDPFEHDVVDKFETKEVSEYCIYDVQSIGYKLEG
HHHHHHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCC
EVIKPARVIVAVKPKENAPNGVKEKVEDKKNPCDENVDSDEQTAEGIDSKEGDK
CEECCEEEEEEECCCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]