Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 160903134

GI number: 160903134

Start: 1858596

End: 1859639

Strand: Direct

Name: carA [H]

Synonym: Pmob_1699

Alternate gene names: 160903134

Gene position: 1858596-1859639 (Clockwise)

Preceding gene: 160903133

Following gene: 160903135

Centisome position: 85.67

GC content: 33.52

Gene sequence:

>1044_bases
ATGAAAGGATTTATAAAGTTAAATGATTCTACCATTTTTGAAGGAGAAATTATCAGTAAAAAAGATTTTGGACAAGGCGA
AGTTGTCTTTAACACGTCTATGACTGGATACGAGGAATCAATAACGGATCCATCTTATGCAGGAGAAATACTGGTAATGA
CCTATCCATTGATAGGAAATTATGGTGTAAATTTAGAGAATTTACAGTCAAAAAGACCCCCCATCAAAGGTTTGATAGTT
AAAGATTACTGTCAATTTCCCTCTCATCACACATCTCAGTATTCACTTTTAGATTATTTAGACCAACATGAAGTACCTAT
TTTAAGTAAAGTAGATACGAGGGCCCTTACGAAAAAGTTAAGAATAGAAGGTAGTATGAATGGTGTGATAACCACCGAGA
AGGATTTTGAAGTTCCAACTTCTGATGAGAATTTACTTGATAGTGTTTCAACAAAGGAAATTTATAGAATTCAAGGGAAC
GGTCCAAAAGTAGCCCTGATAGATTTAGGAGTTAAGAAAAATATAGTGAACCAGCTCAAATCTGAAGGATACGATATTTA
TGTTTTTCCATATAACAGTACTAAAAATGATGTAGATAAGGTTCAACCAGATTTAGTATTATTCTCCAACGGTCCCGGTG
ATCCAAAAAAGGCAAAAGAAGCCATACTTTTAGCTAAAGAGTTTATAGGTGAGAAACCACTTTTTGGGATTTGTTTGGGT
CATCAAATAATCTCTTTGGCGTTAGGTTTTAACACTGTAAAAATGAAATTTGGTCACCGAGGTACCAACCATCCTGTGAT
GAATTTGTTGAACAATAAATGTTATATCACTTCTCAAAACCACGGTTATATGGTTGAAAAAGAGTCAGTGAAAAACAAAG
ATGTAATAATTACCTACATCAATTTGAATGATCAAAGTATCGAGGGAATCATGCACAAAAAGTATCCCATTTTTACTGTC
CAATTTCATCCAGAAGGTGGTCCTGGGGTTCACGATACAACTTTTATTTTTAGAAAAATCAAAGATTTAATCAATAGAAA
CTAA

Upstream 100 bases:

>100_bases
AATGTTTTAAGATTCGTCCCTCCTCTTATAATCACAAAAGAAGAGATAGCCAAGGGTATGAATATTCTCGAAGAAGTTTT
TTTAGAAATGGGGTATTAAG

Downstream 100 bases:

>100_bases
GAGCATACTTCCGGGGAGGTCAACAATGCCAAAAAAACGTGAAATAAAAAATGTTTTAGTTATAGGTTCTGGACCAATAA
TAATAGGTCAAGCAGCAGAA

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MKGFIKLNDSTIFEGEIISKKDFGQGEVVFNTSMTGYEESITDPSYAGEILVMTYPLIGNYGVNLENLQSKRPPIKGLIV
KDYCQFPSHHTSQYSLLDYLDQHEVPILSKVDTRALTKKLRIEGSMNGVITTEKDFEVPTSDENLLDSVSTKEIYRIQGN
GPKVALIDLGVKKNIVNQLKSEGYDIYVFPYNSTKNDVDKVQPDLVLFSNGPGDPKKAKEAILLAKEFIGEKPLFGICLG
HQIISLALGFNTVKMKFGHRGTNHPVMNLLNNKCYITSQNHGYMVEKESVKNKDVIITYINLNDQSIEGIMHKKYPIFTV
QFHPEGGPGVHDTTFIFRKIKDLINRN

Sequences:

>Translated_347_residues
MKGFIKLNDSTIFEGEIISKKDFGQGEVVFNTSMTGYEESITDPSYAGEILVMTYPLIGNYGVNLENLQSKRPPIKGLIV
KDYCQFPSHHTSQYSLLDYLDQHEVPILSKVDTRALTKKLRIEGSMNGVITTEKDFEVPTSDENLLDSVSTKEIYRIQGN
GPKVALIDLGVKKNIVNQLKSEGYDIYVFPYNSTKNDVDKVQPDLVLFSNGPGDPKKAKEAILLAKEFIGEKPLFGICLG
HQIISLALGFNTVKMKFGHRGTNHPVMNLLNNKCYITSQNHGYMVEKESVKNKDVIITYINLNDQSIEGIMHKKYPIFTV
QFHPEGGPGVHDTTFIFRKIKDLINRN
>Mature_347_residues
MKGFIKLNDSTIFEGEIISKKDFGQGEVVFNTSMTGYEESITDPSYAGEILVMTYPLIGNYGVNLENLQSKRPPIKGLIV
KDYCQFPSHHTSQYSLLDYLDQHEVPILSKVDTRALTKKLRIEGSMNGVITTEKDFEVPTSDENLLDSVSTKEIYRIQGN
GPKVALIDLGVKKNIVNQLKSEGYDIYVFPYNSTKNDVDKVQPDLVLFSNGPGDPKKAKEAILLAKEFIGEKPLFGICLG
HQIISLALGFNTVKMKFGHRGTNHPVMNLLNNKCYITSQNHGYMVEKESVKNKDVIITYINLNDQSIEGIMHKKYPIFTV
QFHPEGGPGVHDTTFIFRKIKDLINRN

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=351, Percent_Identity=37.6068376068376, Blast_Score=240, Evalue=1e-63,
Organism=Homo sapiens, GI21361331, Length=359, Percent_Identity=38.4401114206128, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI169790915, Length=359, Percent_Identity=38.4401114206128, Blast_Score=225, Evalue=4e-59,
Organism=Escherichia coli, GI1786215, Length=373, Percent_Identity=36.7292225201072, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI1789760, Length=139, Percent_Identity=28.7769784172662, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193204318, Length=369, Percent_Identity=40.3794037940379, Blast_Score=246, Evalue=2e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=356, Percent_Identity=40.4494382022472, Blast_Score=244, Evalue=1e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=385, Percent_Identity=38.1818181818182, Blast_Score=232, Evalue=6e-62,
Organism=Drosophila melanogaster, GI45555749, Length=366, Percent_Identity=40.1639344262295, Blast_Score=246, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24642586, Length=366, Percent_Identity=40.1639344262295, Blast_Score=246, Evalue=2e-65,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 39042; Mature: 39042

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFIKLNDSTIFEGEIISKKDFGQGEVVFNTSMTGYEESITDPSYAGEILVMTYPLIGN
CCCEEEECCCEEECCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCCEEEEEEEEEECC
YGVNLENLQSKRPPIKGLIVKDYCQFPSHHTSQYSLLDYLDQHEVPILSKVDTRALTKKL
CCCCHHHHHHCCCCCCEEEEEHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHE
RIEGSMNGVITTEKDFEVPTSDENLLDSVSTKEIYRIQGNGPKVALIDLGVKKNIVNQLK
EECCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHH
SEGYDIYVFPYNSTKNDVDKVQPDLVLFSNGPGDPKKAKEAILLAKEFIGEKPLFGICLG
HCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHH
HQIISLALGFNTVKMKFGHRGTNHPVMNLLNNKCYITSQNHGYMVEKESVKNKDVIITYI
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCEEEEEECCCCEEEECCCCCCCCEEEEEE
NLNDQSIEGIMHKKYPIFTVQFHPEGGPGVHDTTFIFRKIKDLINRN
ECCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKGFIKLNDSTIFEGEIISKKDFGQGEVVFNTSMTGYEESITDPSYAGEILVMTYPLIGN
CCCEEEECCCEEECCEEECCCCCCCCEEEEECCCCCCHHHCCCCCCCCEEEEEEEEEECC
YGVNLENLQSKRPPIKGLIVKDYCQFPSHHTSQYSLLDYLDQHEVPILSKVDTRALTKKL
CCCCHHHHHHCCCCCCEEEEEHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHE
RIEGSMNGVITTEKDFEVPTSDENLLDSVSTKEIYRIQGNGPKVALIDLGVKKNIVNQLK
EECCCCCCEEEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHH
SEGYDIYVFPYNSTKNDVDKVQPDLVLFSNGPGDPKKAKEAILLAKEFIGEKPLFGICLG
HCCCEEEEEECCCCCCHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCEEEEHHH
HQIISLALGFNTVKMKFGHRGTNHPVMNLLNNKCYITSQNHGYMVEKESVKNKDVIITYI
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHCCEEEEEECCCCEEEECCCCCCCCEEEEEE
NLNDQSIEGIMHKKYPIFTVQFHPEGGPGVHDTTFIFRKIKDLINRN
ECCCHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]