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The map label for this gene is asnS [H]

Identifier: 160903095

GI number: 160903095

Start: 1815976

End: 1816935

Strand: Direct

Name: asnS [H]

Synonym: Pmob_1659

Alternate gene names: 160903095

Gene position: 1815976-1816935 (Clockwise)

Preceding gene: 160903086

Following gene: 160903096

Centisome position: 83.7

GC content: 34.48

Gene sequence:

>960_bases
ATGGCAGAAGTAGAGAAGCAGGATAGAAGAGTGGATTCTGTGGAGCTAGTAAAAACTTATTTGGAGGACGAGACTTACAG
TGACGCGCTTTTAGTTCAATCAGAGATACTGAGATTATCGAGGGAATTTCTTGCAGAGAAGGGTTTTGTTGAAATTCTAC
CAGTGATTGTCTCTACGATCACCGATCCTTTAAATCACGACGTTTTTGAAGCACAAATTGATTATTACGGTAACAAATAT
TACGTTACTAAATCTATGATTCTGCAAAAACAAGTTAGTGTTTTGATTCATGATAAGATATTTTCCTTTTCGCCAAATTT
AAGGTTGGAAACAGCAGAAAAATATTCTTCTGGTAGACACTTGATTGATTTTGTTCAACTAGATATAGAAGCTAAAGAAA
CTAAAAGAGAAGAGATTATGGATTTGATGGAAGATTTAATAATTTATGTACTAAAAGGTATCTTGACGAAATATTCTGGT
ATAATAGAAAAGTATCACCCAAATTTGACTGTTCCTAAAAAGCCTTTTCAAAAGATAAGCGTAAAAAAGGCAAAAGAGCT
TTACGGTGATGATTATGAAAAGATTTTATCTGAACGAGCTGAAGGGCCTGTGTGGTTAATTGATATTCCGCTCTTGAACA
GGGAGTTTTATGATAAACAAGATATGGATAATCCTGATGTATTGTTGGATTTTGACCTGATATATCCTGAGGGTCATGGG
GAAGGCATATCTGGTGGCGAAAGAGAACACGAGTATGAGCAAATAGTGAAAAGAATCAAATTGAAAGGTAACGGGCTGGA
AAGCTATGAAGATTATTTAAAATTGGCAAAAGAAGGGTTATTAAAACACTCAGCAGGATGTGGTATAGGTATAGAAAGAT
TCACTAAATACATATTAGATTTGGATCATGTTGAAAAAAGTAGATTGTTTGCTAAAGCACCTGGAAAGTATACCATTTAG

Upstream 100 bases:

>100_bases
TTTTGAAAATTACCGACGCAGAAAAAATAGCGTCGGTTTTTTTATTTGTATTTTAAAAGACGATATACCGTCTTTAAAAA
ACACTACAGGAGGGAAAATT

Downstream 100 bases:

>100_bases
TTGTGTTGATTAAAAAACCTAGAAAATGTAACTCAAAGCAAGAGACGTAAATGTTGAACGTCTCTGCTTTTCCTTTCATA
AAAATTAACTTATTTTTCAC

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MAEVEKQDRRVDSVELVKTYLEDETYSDALLVQSEILRLSREFLAEKGFVEILPVIVSTITDPLNHDVFEAQIDYYGNKY
YVTKSMILQKQVSVLIHDKIFSFSPNLRLETAEKYSSGRHLIDFVQLDIEAKETKREEIMDLMEDLIIYVLKGILTKYSG
IIEKYHPNLTVPKKPFQKISVKKAKELYGDDYEKILSERAEGPVWLIDIPLLNREFYDKQDMDNPDVLLDFDLIYPEGHG
EGISGGEREHEYEQIVKRIKLKGNGLESYEDYLKLAKEGLLKHSAGCGIGIERFTKYILDLDHVEKSRLFAKAPGKYTI

Sequences:

>Translated_319_residues
MAEVEKQDRRVDSVELVKTYLEDETYSDALLVQSEILRLSREFLAEKGFVEILPVIVSTITDPLNHDVFEAQIDYYGNKY
YVTKSMILQKQVSVLIHDKIFSFSPNLRLETAEKYSSGRHLIDFVQLDIEAKETKREEIMDLMEDLIIYVLKGILTKYSG
IIEKYHPNLTVPKKPFQKISVKKAKELYGDDYEKILSERAEGPVWLIDIPLLNREFYDKQDMDNPDVLLDFDLIYPEGHG
EGISGGEREHEYEQIVKRIKLKGNGLESYEDYLKLAKEGLLKHSAGCGIGIERFTKYILDLDHVEKSRLFAKAPGKYTI
>Mature_318_residues
AEVEKQDRRVDSVELVKTYLEDETYSDALLVQSEILRLSREFLAEKGFVEILPVIVSTITDPLNHDVFEAQIDYYGNKYY
VTKSMILQKQVSVLIHDKIFSFSPNLRLETAEKYSSGRHLIDFVQLDIEAKETKREEIMDLMEDLIIYVLKGILTKYSGI
IEKYHPNLTVPKKPFQKISVKKAKELYGDDYEKILSERAEGPVWLIDIPLLNREFYDKQDMDNPDVLLDFDLIYPEGHGE
GISGGEREHEYEQIVKRIKLKGNGLESYEDYLKLAKEGLLKHSAGCGIGIERFTKYILDLDHVEKSRLFAKAPGKYTI

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI45439306, Length=341, Percent_Identity=30.2052785923754, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI4758762, Length=312, Percent_Identity=23.7179487179487, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI226491502, Length=325, Percent_Identity=26.4615384615385, Blast_Score=79, Evalue=6e-15,
Organism=Escherichia coli, GI1787161, Length=268, Percent_Identity=25, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71984122, Length=330, Percent_Identity=23.9393939393939, Blast_Score=89, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17551876, Length=319, Percent_Identity=26.9592476489028, Blast_Score=84, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6319872, Length=289, Percent_Identity=28.3737024221453, Blast_Score=87, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6323011, Length=315, Percent_Identity=26.3492063492064, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6321807, Length=319, Percent_Identity=22.884012539185, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17136276, Length=339, Percent_Identity=26.5486725663717, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19921528, Length=312, Percent_Identity=21.7948717948718, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 36911; Mature: 36780

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEVEKQDRRVDSVELVKTYLEDETYSDALLVQSEILRLSREFLAEKGFVEILPVIVSTI
CCCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TDPLNHDVFEAQIDYYGNKYYVTKSMILQKQVSVLIHDKIFSFSPNLRLETAEKYSSGRH
HCHHCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCC
LIDFVQLDIEAKETKREEIMDLMEDLIIYVLKGILTKYSGIIEKYHPNLTVPKKPFQKIS
EEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VKKAKELYGDDYEKILSERAEGPVWLIDIPLLNREFYDKQDMDNPDVLLDFDLIYPEGHG
HHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCCCCEEEEEEEECCCCCC
EGISGGEREHEYEQIVKRIKLKGNGLESYEDYLKLAKEGLLKHSAGCGIGIERFTKYILD
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LDHVEKSRLFAKAPGKYTI
HHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
AEVEKQDRRVDSVELVKTYLEDETYSDALLVQSEILRLSREFLAEKGFVEILPVIVSTI
CCCCCHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
TDPLNHDVFEAQIDYYGNKYYVTKSMILQKQVSVLIHDKIFSFSPNLRLETAEKYSSGRH
HCHHCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHCCCCC
LIDFVQLDIEAKETKREEIMDLMEDLIIYVLKGILTKYSGIIEKYHPNLTVPKKPFQKIS
EEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VKKAKELYGDDYEKILSERAEGPVWLIDIPLLNREFYDKQDMDNPDVLLDFDLIYPEGHG
HHHHHHHCCCCHHHHHHHCCCCCEEEEECCCCCCHHCCCCCCCCCCEEEEEEEECCCCCC
EGISGGEREHEYEQIVKRIKLKGNGLESYEDYLKLAKEGLLKHSAGCGIGIERFTKYILD
CCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
LDHVEKSRLFAKAPGKYTI
HHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12622808 [H]