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The map label for this gene is appF [H]

Identifier: 160903017

GI number: 160903017

Start: 1720925

End: 1722103

Strand: Direct

Name: appF [H]

Synonym: Pmob_1579

Alternate gene names: 160903017

Gene position: 1720925-1722103 (Clockwise)

Preceding gene: 160903016

Following gene: 160903021

Centisome position: 79.32

GC content: 35.96

Gene sequence:

>1179_bases
GTGCCAAATAGTGACAAAGTGATCTTAAAAGTTGAAAATTTGAAAAAATATTTTCCTGTTAGAGCCGGGGTCTTCAAAAG
AACAGTCGCCCAAGTGCTAGCTGTAGACGATATTAGTTTTGAAATAAAAGAAGGCGAAACTCTGGGGCTTGTTGGTGAGT
CCGGTTGTGGTAAAAGTACAACAGGCATGACAATCCTGAGATTATACGAACCAACAGATGGACGAATTATAATGGAGGAA
CAAGACACTACACCATGGTTCATGAAAGGTACCACCGTTAATAAGTATGTTAAGAAGATTTATTCAGACAGATTTGAAAA
AATGAAAAAAGAATTAGGTTCAGAAGAAGAAGTAATTAAGAAATTAGATAATCAAATAGACAAAAAATACGCACAGTTAT
ATTTTCAAAACGGAGTAAGTGAAATAAGAAAAGATTTGTTGAATAATTTGGCTGAAAAAAGACGATCCTTTAGAAAAAAT
GCCCAAGTCATCTTTCAAGACCCCTATTCTTCCTTAAATCCAAGAATGAGAGTTCTGGATATCATAGGAGAAGGTATGAA
AGTTAATAAAATGGGTACAGCTTCTGAAATAAGAGATAGAGTAGCTAACTTAATGGAAACAGTAGGATTATCGAAAGATT
ATGTTTATAGGTATCCCCACCAATTTTCTGGTGGACAAAGGCAAAGAGTAGGGATAGCGCGTGCTTTGGCATTGGACCCA
AAATTAATCATTTCTGATGAAGCAGTTTCCGCTTTAGATGTTTCCATTCAATCTCAGATAATAAATTTAATGGTTGACTT
GAAAAATGATTACGGTTTAACCTATGTTTTTATAGCTCACGATTTGGCCGTTGTGAAGCACATAAGCGACAGAATAGCGG
TTATGTATCTAGGAAAGATAGCTGAATTAACAACGAAAAAAGAGCTCTTTGATGAGCCATTACACCCTTATACGGTTTCT
TTGATGTCGGCAATTCCTATACCAGATCCGGAAGTGAAAAAGAAAAGAGTGGTTTTACAAGGTGATGTACCAAGCCCTCT
AAATCCACCCTCGGGTTGTAGATTTCATCCTCGTTGTCCAATTGCTAAAGATATTTGTAGCAAAGAAGAACCACCACTTA
AAGAAGTTAAGCCAGGACATTTTGTATCATGTCATTTTCCTGGTGAGTTTAAAATTTAA

Upstream 100 bases:

>100_bases
ATCCTAACTTGGAAGAAGTAGAACCTCGCCATTTTTCCCGTTGTTGGCACATAGACAAATTATTGGACGAAATGAACAAA
GTCAAAGAAGGTGAAGTATA

Downstream 100 bases:

>100_bases
AAGCTTGGGTTTTACCCAAGCTTTTAAATACTTTAGATTTCTCCTTTTTCGTGCAACTTTAGATCTATTTTCTTCTCTAA
CTTTTGTAGTATGACTGGTA

Product: oligopeptide/dipeptide ABC transporter ATPase

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MPNSDKVILKVENLKKYFPVRAGVFKRTVAQVLAVDDISFEIKEGETLGLVGESGCGKSTTGMTILRLYEPTDGRIIMEE
QDTTPWFMKGTTVNKYVKKIYSDRFEKMKKELGSEEEVIKKLDNQIDKKYAQLYFQNGVSEIRKDLLNNLAEKRRSFRKN
AQVIFQDPYSSLNPRMRVLDIIGEGMKVNKMGTASEIRDRVANLMETVGLSKDYVYRYPHQFSGGQRQRVGIARALALDP
KLIISDEAVSALDVSIQSQIINLMVDLKNDYGLTYVFIAHDLAVVKHISDRIAVMYLGKIAELTTKKELFDEPLHPYTVS
LMSAIPIPDPEVKKKRVVLQGDVPSPLNPPSGCRFHPRCPIAKDICSKEEPPLKEVKPGHFVSCHFPGEFKI

Sequences:

>Translated_392_residues
MPNSDKVILKVENLKKYFPVRAGVFKRTVAQVLAVDDISFEIKEGETLGLVGESGCGKSTTGMTILRLYEPTDGRIIMEE
QDTTPWFMKGTTVNKYVKKIYSDRFEKMKKELGSEEEVIKKLDNQIDKKYAQLYFQNGVSEIRKDLLNNLAEKRRSFRKN
AQVIFQDPYSSLNPRMRVLDIIGEGMKVNKMGTASEIRDRVANLMETVGLSKDYVYRYPHQFSGGQRQRVGIARALALDP
KLIISDEAVSALDVSIQSQIINLMVDLKNDYGLTYVFIAHDLAVVKHISDRIAVMYLGKIAELTTKKELFDEPLHPYTVS
LMSAIPIPDPEVKKKRVVLQGDVPSPLNPPSGCRFHPRCPIAKDICSKEEPPLKEVKPGHFVSCHFPGEFKI
>Mature_391_residues
PNSDKVILKVENLKKYFPVRAGVFKRTVAQVLAVDDISFEIKEGETLGLVGESGCGKSTTGMTILRLYEPTDGRIIMEEQ
DTTPWFMKGTTVNKYVKKIYSDRFEKMKKELGSEEEVIKKLDNQIDKKYAQLYFQNGVSEIRKDLLNNLAEKRRSFRKNA
QVIFQDPYSSLNPRMRVLDIIGEGMKVNKMGTASEIRDRVANLMETVGLSKDYVYRYPHQFSGGQRQRVGIARALALDPK
LIISDEAVSALDVSIQSQIINLMVDLKNDYGLTYVFIAHDLAVVKHISDRIAVMYLGKIAELTTKKELFDEPLHPYTVSL
MSAIPIPDPEVKKKRVVLQGDVPSPLNPPSGCRFHPRCPIAKDICSKEEPPLKEVKPGHFVSCHFPGEFKI

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG4608

COG function: function code E; ABC-type oligopeptide transport system, ATPase component

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI42490749, Length=138, Percent_Identity=35.5072463768116, Blast_Score=67, Evalue=4e-11,
Organism=Homo sapiens, GI171184400, Length=132, Percent_Identity=36.3636363636364, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1787500, Length=384, Percent_Identity=42.4479166666667, Blast_Score=287, Evalue=9e-79,
Organism=Escherichia coli, GI1789962, Length=380, Percent_Identity=37.6315789473684, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI145693107, Length=377, Percent_Identity=37.4005305039788, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1787758, Length=213, Percent_Identity=44.131455399061, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI48994883, Length=245, Percent_Identity=40, Blast_Score=188, Evalue=5e-49,
Organism=Escherichia coli, GI1789963, Length=246, Percent_Identity=42.6829268292683, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787548, Length=376, Percent_Identity=30.8510638297872, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1787547, Length=170, Percent_Identity=40.5882352941176, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1788506, Length=168, Percent_Identity=43.452380952381, Blast_Score=146, Evalue=2e-36,
Organism=Escherichia coli, GI1787759, Length=283, Percent_Identity=30.3886925795053, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789891, Length=150, Percent_Identity=41.3333333333333, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1789032, Length=177, Percent_Identity=37.8531073446328, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994997, Length=208, Percent_Identity=29.3269230769231, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1787370, Length=155, Percent_Identity=37.4193548387097, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786398, Length=143, Percent_Identity=40.5594405594406, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1787712, Length=187, Percent_Identity=31.5508021390374, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789672, Length=319, Percent_Identity=24.4514106583072, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI87081791, Length=166, Percent_Identity=33.1325301204819, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI1786872, Length=116, Percent_Identity=39.6551724137931, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1788225, Length=183, Percent_Identity=33.879781420765, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1788761, Length=174, Percent_Identity=31.0344827586207, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788644, Length=163, Percent_Identity=32.5153374233129, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1787029, Length=159, Percent_Identity=32.7044025157233, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1790162, Length=309, Percent_Identity=26.537216828479, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788450, Length=123, Percent_Identity=33.3333333333333, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI87081709, Length=166, Percent_Identity=29.5180722891566, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1787089, Length=160, Percent_Identity=33.125, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1789890, Length=164, Percent_Identity=32.3170731707317, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI87082267, Length=196, Percent_Identity=29.5918367346939, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789864, Length=282, Percent_Identity=23.758865248227, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1787164, Length=145, Percent_Identity=31.7241379310345, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790467, Length=167, Percent_Identity=28.1437125748503, Blast_Score=72, Evalue=8e-14,
Organism=Escherichia coli, GI87081834, Length=146, Percent_Identity=29.4520547945205, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1786563, Length=137, Percent_Identity=34.3065693430657, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1786703, Length=156, Percent_Identity=28.8461538461538, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786345, Length=146, Percent_Identity=28.7671232876712, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1789873, Length=108, Percent_Identity=33.3333333333333, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI193209708, Length=90, Percent_Identity=50, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25146777, Length=127, Percent_Identity=40.9448818897638, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17558664, Length=92, Percent_Identity=40.2173913043478, Blast_Score=68, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=131, Percent_Identity=32.824427480916, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324985, Length=129, Percent_Identity=34.8837209302326, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6323217, Length=93, Percent_Identity=38.7096774193548, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI85726357, Length=99, Percent_Identity=38.3838383838384, Blast_Score=69, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593
- InterPro:   IPR010066
- InterPro:   IPR013563 [H]

Pfam domain/function: PF00005 ABC_tran; PF08352 oligo_HPY [H]

EC number: NA

Molecular weight: Translated: 44357; Mature: 44226

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSDKVILKVENLKKYFPVRAGVFKRTVAQVLAVDDISFEIKEGETLGLVGESGCGKST
CCCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
TGMTILRLYEPTDGRIIMEEQDTTPWFMKGTTVNKYVKKIYSDRFEKMKKELGSEEEVIK
CCEEEEEEEECCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KLDNQIDKKYAQLYFQNGVSEIRKDLLNNLAEKRRSFRKNAQVIFQDPYSSLNPRMRVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
IIGEGMKVNKMGTASEIRDRVANLMETVGLSKDYVYRYPHQFSGGQRQRVGIARALALDP
HHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCC
KLIISDEAVSALDVSIQSQIINLMVDLKNDYGLTYVFIAHDLAVVKHISDRIAVMYLGKI
CEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AELTTKKELFDEPLHPYTVSLMSAIPIPDPEVKKKRVVLQGDVPSPLNPPSGCRFHPRCP
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
IAKDICSKEEPPLKEVKPGHFVSCHFPGEFKI
CHHHHHCCCCCCHHHCCCCCEEEEECCCCCCC
>Mature Secondary Structure 
PNSDKVILKVENLKKYFPVRAGVFKRTVAQVLAVDDISFEIKEGETLGLVGESGCGKST
CCCCEEEEEECCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCEEEEECCCCCCCCC
TGMTILRLYEPTDGRIIMEEQDTTPWFMKGTTVNKYVKKIYSDRFEKMKKELGSEEEVIK
CCEEEEEEEECCCCEEEEECCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHH
KLDNQIDKKYAQLYFQNGVSEIRKDLLNNLAEKRRSFRKNAQVIFQDPYSSLNPRMRVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
IIGEGMKVNKMGTASEIRDRVANLMETVGLSKDYVYRYPHQFSGGQRQRVGIARALALDP
HHCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCHHHHHHHHHHHCCC
KLIISDEAVSALDVSIQSQIINLMVDLKNDYGLTYVFIAHDLAVVKHISDRIAVMYLGKI
CEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
AELTTKKELFDEPLHPYTVSLMSAIPIPDPEVKKKRVVLQGDVPSPLNPPSGCRFHPRCP
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCC
IAKDICSKEEPPLKEVKPGHFVSCHFPGEFKI
CHHHHHCCCCCCHHHCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]