Click here to switch to the map view.

The map label for this gene is mnmE

Identifier: 160902854

GI number: 160902854

Start: 1514684

End: 1516042

Strand: Direct

Name: mnmE

Synonym: Pmob_1409

Alternate gene names: 160902854

Gene position: 1514684-1516042 (Clockwise)

Preceding gene: 160902853

Following gene: 160902855

Centisome position: 69.82

GC content: 33.85

Gene sequence:

>1359_bases
GTGTTGAATGATACAATAGTAGCCATTTCATCTCCGATTGGTACGGGAGCCATAGGGGTAGTAAGAATTTCTGGCGATCA
CGTTAAAAACATAATAGACCAAGCATTAAAAAGAAAAAAATATACACCTAAGAAGATGTACTATGGTTGGCTTTACGATA
AAGAAGGAGAAAAAGTCGACGAGATTACCTGGGTTTACCATTCACAGCCTCACTCTTATACAGGGGAGGATATGCTTGAA
ATATTTTGCCATGGTGGTAAACTTATCACTTATGCTGTACTCAATACGATTATAAAATATGGGGCAAGACAAGCTTTACC
TGGGGAGTTTACAAAACGTGCTGTCTTAAACGGTAAGATGGATCTCATAAAAGCAGAAGCTGTAAACAATGTGATTACTT
CAGAGACGGAAATCTCTTTAAAAGCGTCTTTTAATCAACTTAAAAATGCCGTTTCGGAAAAAATTAAAGACATAAAAAAC
AGTTTGCTAAATATCTCAGCCCAGATCGAGGTAGAAATGGATTATCCTGATGACATAGAATTTGAAGATCACGATTTAAA
AAACAAATTAGTCCATATCGTAAATAGTATGAATCAACTACTAAAAGAAGCTGAGAACGGTATTATAGCCGTTGAAGGTG
TAAGAACAGTTATAGTAGGAAAGCCGAACTCTGGGAAAAGTACCTTGTTAAACGCCCTTTTGAGAAAAGATAGAGCGATT
GTAACCGATATTCCTGGGACAACTAGGGACACAATAGAAGAAAACTTGAACATAAACGGCATATACATAAGGCTCATAGA
TACAGCCGGAATACGATATACAGAAGATACCTTGGAAAGGGTTGGAATAGAAAGGACCATAAATTCTATAAAGAATTCTC
ATCTAATCTTGTTTGTTTTGGATGGTACAACGCCTTTCACCCAGGAAGACGAACTAATTTATAATAAATTAAATGAATTG
GGAGATAAAACTGTTATAATTATATTAAATAAATCCGACTCTCCTAATTTTACAGAGAATAATTATCTATCTTTGAAACA
AAAAAATCCAAATGATTTTGTTATAATTTCTGCAAAGAACGGAGCAATTAAAAATTTGGAAAATAAGATTTATGAAAAAT
TTTTTGAGAAGGTCAACATAGAAGAACCAACTCTAACTAACCAAAGGCAAAAGATAACTTTAGAATCATCTAAAGAGTTT
GTTTTAAACGCCATTAATTCGCTTGAGAAAGGTTTTTCTAACGATATAATTATGTATGACGTAAGAAAAGCACTTGAAAA
GATCTACGAACTCTCTGGGGAAAATTATACTGAAGAATTACTGGATAAAATCTTCTCCACATTCTGTGTTGGAAAGTAG

Upstream 100 bases:

>100_bases
AAAGATTACCTTTTATCCAGAAACGAAAGAACTCATTAGTTATGGGCCAAAAGATCATATGAAATATATGGAAAAGGTTT
TTTCTTAAGAGGTGAAAAAT

Downstream 100 bases:

>100_bases
GAGGTAAAATATATGTATCTGTTAGTTTCAATTTTTTTTGGACTAATCCCCTTGATACAATTTTTCATAAAAGGTTGGTA
CTTTTTTGGACTATCTTCTA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MLNDTIVAISSPIGTGAIGVVRISGDHVKNIIDQALKRKKYTPKKMYYGWLYDKEGEKVDEITWVYHSQPHSYTGEDMLE
IFCHGGKLITYAVLNTIIKYGARQALPGEFTKRAVLNGKMDLIKAEAVNNVITSETEISLKASFNQLKNAVSEKIKDIKN
SLLNISAQIEVEMDYPDDIEFEDHDLKNKLVHIVNSMNQLLKEAENGIIAVEGVRTVIVGKPNSGKSTLLNALLRKDRAI
VTDIPGTTRDTIEENLNINGIYIRLIDTAGIRYTEDTLERVGIERTINSIKNSHLILFVLDGTTPFTQEDELIYNKLNEL
GDKTVIIILNKSDSPNFTENNYLSLKQKNPNDFVIISAKNGAIKNLENKIYEKFFEKVNIEEPTLTNQRQKITLESSKEF
VLNAINSLEKGFSNDIIMYDVRKALEKIYELSGENYTEELLDKIFSTFCVGK

Sequences:

>Translated_452_residues
MLNDTIVAISSPIGTGAIGVVRISGDHVKNIIDQALKRKKYTPKKMYYGWLYDKEGEKVDEITWVYHSQPHSYTGEDMLE
IFCHGGKLITYAVLNTIIKYGARQALPGEFTKRAVLNGKMDLIKAEAVNNVITSETEISLKASFNQLKNAVSEKIKDIKN
SLLNISAQIEVEMDYPDDIEFEDHDLKNKLVHIVNSMNQLLKEAENGIIAVEGVRTVIVGKPNSGKSTLLNALLRKDRAI
VTDIPGTTRDTIEENLNINGIYIRLIDTAGIRYTEDTLERVGIERTINSIKNSHLILFVLDGTTPFTQEDELIYNKLNEL
GDKTVIIILNKSDSPNFTENNYLSLKQKNPNDFVIISAKNGAIKNLENKIYEKFFEKVNIEEPTLTNQRQKITLESSKEF
VLNAINSLEKGFSNDIIMYDVRKALEKIYELSGENYTEELLDKIFSTFCVGK
>Mature_452_residues
MLNDTIVAISSPIGTGAIGVVRISGDHVKNIIDQALKRKKYTPKKMYYGWLYDKEGEKVDEITWVYHSQPHSYTGEDMLE
IFCHGGKLITYAVLNTIIKYGARQALPGEFTKRAVLNGKMDLIKAEAVNNVITSETEISLKASFNQLKNAVSEKIKDIKN
SLLNISAQIEVEMDYPDDIEFEDHDLKNKLVHIVNSMNQLLKEAENGIIAVEGVRTVIVGKPNSGKSTLLNALLRKDRAI
VTDIPGTTRDTIEENLNINGIYIRLIDTAGIRYTEDTLERVGIERTINSIKNSHLILFVLDGTTPFTQEDELIYNKLNEL
GDKTVIIILNKSDSPNFTENNYLSLKQKNPNDFVIISAKNGAIKNLENKIYEKFFEKVNIEEPTLTNQRQKITLESSKEF
VLNAINSLEKGFSNDIIMYDVRKALEKIYELSGENYTEELLDKIFSTFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=468, Percent_Identity=27.991452991453, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI193082993, Length=460, Percent_Identity=27.8260869565217, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI193082988, Length=502, Percent_Identity=26.6932270916335, Blast_Score=166, Evalue=6e-41,
Organism=Escherichia coli, GI2367268, Length=458, Percent_Identity=34.061135371179, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI87082120, Length=125, Percent_Identity=35.2, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788919, Length=150, Percent_Identity=36, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17507259, Length=464, Percent_Identity=34.051724137931, Blast_Score=224, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6323665, Length=493, Percent_Identity=31.0344827586207, Blast_Score=210, Evalue=4e-55,
Organism=Drosophila melanogaster, GI45550813, Length=478, Percent_Identity=30.3347280334728, Blast_Score=195, Evalue=5e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_PETMO (A9BHZ7)

Other databases:

- EMBL:   CP000879
- RefSeq:   YP_001568435.1
- ProteinModelPortal:   A9BHZ7
- SMR:   A9BHZ7
- GeneID:   5756807
- GenomeReviews:   CP000879_GR
- KEGG:   pmo:Pmob_1409
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- BioCyc:   PMOB403833:PMOB_1409-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51067; Mature: 51067

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 80-80 BINDING 119-119 BINDING 452-452

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNDTIVAISSPIGTGAIGVVRISGDHVKNIIDQALKRKKYTPKKMYYGWLYDKEGEKVD
CCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
EITWVYHSQPHSYTGEDMLEIFCHGGKLITYAVLNTIIKYGARQALPGEFTKRAVLNGKM
CEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCH
DLIKAEAVNNVITSETEISLKASFNQLKNAVSEKIKDIKNSLLNISAQIEVEMDYPDDIE
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCC
FEDHDLKNKLVHIVNSMNQLLKEAENGIIAVEGVRTVIVGKPNSGKSTLLNALLRKDRAI
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEECCCCCHHHHHHHHHHCCCEE
VTDIPGTTRDTIEENLNINGIYIRLIDTAGIRYTEDTLERVGIERTINSIKNSHLILFVL
EECCCCCCHHHHHHCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHHHHCCCEEEEEEE
DGTTPFTQEDELIYNKLNELGDKTVIIILNKSDSPNFTENNYLSLKQKNPNDFVIISAKN
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECC
GAIKNLENKIYEKFFEKVNIEEPTLTNQRQKITLESSKEFVLNAINSLEKGFSNDIIMYD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEHH
VRKALEKIYELSGENYTEELLDKIFSTFCVGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNDTIVAISSPIGTGAIGVVRISGDHVKNIIDQALKRKKYTPKKMYYGWLYDKEGEKVD
CCCCEEEEEECCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCC
EITWVYHSQPHSYTGEDMLEIFCHGGKLITYAVLNTIIKYGARQALPGEFTKRAVLNGKM
CEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCH
DLIKAEAVNNVITSETEISLKASFNQLKNAVSEKIKDIKNSLLNISAQIEVEMDYPDDIE
HHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEEEECCCCCCC
FEDHDLKNKLVHIVNSMNQLLKEAENGIIAVEGVRTVIVGKPNSGKSTLLNALLRKDRAI
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECEEEEEEECCCCCHHHHHHHHHHCCCEE
VTDIPGTTRDTIEENLNINGIYIRLIDTAGIRYTEDTLERVGIERTINSIKNSHLILFVL
EECCCCCCHHHHHHCCCCCEEEEEEEECCCCEECHHHHHHHHHHHHHHHHCCCEEEEEEE
DGTTPFTQEDELIYNKLNELGDKTVIIILNKSDSPNFTENNYLSLKQKNPNDFVIISAKN
CCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEEEEECCCCCEEEEEECC
GAIKNLENKIYEKFFEKVNIEEPTLTNQRQKITLESSKEFVLNAINSLEKGFSNDIIMYD
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCEEEHH
VRKALEKIYELSGENYTEELLDKIFSTFCVGK
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA