Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 160902849

GI number: 160902849

Start: 1509519

End: 1510214

Strand: Direct

Name: fliA [H]

Synonym: Pmob_1404

Alternate gene names: 160902849

Gene position: 1509519-1510214 (Clockwise)

Preceding gene: 160902848

Following gene: 160902850

Centisome position: 69.58

GC content: 30.75

Gene sequence:

>696_bases
ATGAAATACAAAATTAATGAAGAACAACTGGTAATGGAGTTTTTGCCAAAAATAAAGATTATAGCTTTGAATCTGAAAAC
CACTTTACCAAAAAATATAGAATTAGAGGATTTGATTCAGGAGGGGATTATTGGATTACTTCAATCATATAAAAGATACA
ACCCGGAAAAAGGTACTTCCTTTTACACCTACGCTCTAAAAAGGATAAAAGGTTCAATGTATGATTATCTGAGGAGAATA
GATTGGCTTCCAAAGGACGTTCGAAGTTTAGTAAAAAAGTACGAAGATTTGATTTACGAATGTAAAGATAATGAGTACAT
AGACGATAATTCAGTGGCAGAAAAACTTCATATTGAAAAAAATGATGTAGATAAAATAAAGTTCTCCTTGAGCAAAAGGC
AGATTCTTCAATTGGATGAGTACTTTTTAAACAATGAAGAAGATACCTGGCTTGAAGATATACAAGAAGAAAACGACCCT
GAAATACTGGCTTATAAAGATATGTTACATGATAAATTAACAGAAAGTATCGAGAAATTGGAAGAAAGAGAAAAATTGAT
ACTTTCTCTATACTATAACGAAGGCTTAACCTTTAAAGAAATAGGAAGTGTTTTAGAAATAAGTGAATCACGGGTTTCCC
AGTTACACTCAGTAATATTGGTGAAATTAAAAAAGATGATTCAAGGAAGTGAATAA

Upstream 100 bases:

>100_bases
TAGAAGTGTTGAATTTAACATCGCTAATGGAGAATTAAAAGTTAAGAAGGTCGGCGGGGGAGAAAAAGTTGAAATCGCAG
TAATTTAAGGTGGCAAATAT

Downstream 100 bases:

>100_bases
ATATGGCTTCACTTCATGAGTTACAAATATTAGTGACCAAATCTATAGATGTAGCAAACAATGTCCAGGCGATATCTCAT
TCTTATGATGCAGCTAAAAA

Product: RNA polymerase sigma-28 subunit FliA/WhiG

Products: NA

Alternate protein names: Sigma-27; Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MKYKINEEQLVMEFLPKIKIIALNLKTTLPKNIELEDLIQEGIIGLLQSYKRYNPEKGTSFYTYALKRIKGSMYDYLRRI
DWLPKDVRSLVKKYEDLIYECKDNEYIDDNSVAEKLHIEKNDVDKIKFSLSKRQILQLDEYFLNNEEDTWLEDIQEENDP
EILAYKDMLHDKLTESIEKLEEREKLILSLYYNEGLTFKEIGSVLEISESRVSQLHSVILVKLKKMIQGSE

Sequences:

>Translated_231_residues
MKYKINEEQLVMEFLPKIKIIALNLKTTLPKNIELEDLIQEGIIGLLQSYKRYNPEKGTSFYTYALKRIKGSMYDYLRRI
DWLPKDVRSLVKKYEDLIYECKDNEYIDDNSVAEKLHIEKNDVDKIKFSLSKRQILQLDEYFLNNEEDTWLEDIQEENDP
EILAYKDMLHDKLTESIEKLEEREKLILSLYYNEGLTFKEIGSVLEISESRVSQLHSVILVKLKKMIQGSE
>Mature_231_residues
MKYKINEEQLVMEFLPKIKIIALNLKTTLPKNIELEDLIQEGIIGLLQSYKRYNPEKGTSFYTYALKRIKGSMYDYLRRI
DWLPKDVRSLVKKYEDLIYECKDNEYIDDNSVAEKLHIEKNDVDKIKFSLSKRQILQLDEYFLNNEEDTWLEDIQEENDP
EILAYKDMLHDKLTESIEKLEEREKLILSLYYNEGLTFKEIGSVLEISESRVSQLHSVILVKLKKMIQGSE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for class 3 flagellar operons [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=38.3928571428571, Blast_Score=149, Evalue=1e-37,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27457; Mature: 27457

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYKINEEQLVMEFLPKIKIIALNLKTTLPKNIELEDLIQEGIIGLLQSYKRYNPEKGTS
CCCCCCHHHHHHHHCCCEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
FYTYALKRIKGSMYDYLRRIDWLPKDVRSLVKKYEDLIYECKDNEYIDDNSVAEKLHIEK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
NDVDKIKFSLSKRQILQLDEYFLNNEEDTWLEDIQEENDPEILAYKDMLHDKLTESIEKL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
EEREKLILSLYYNEGLTFKEIGSVLEISESRVSQLHSVILVKLKKMIQGSE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKYKINEEQLVMEFLPKIKIIALNLKTTLPKNIELEDLIQEGIIGLLQSYKRYNPEKGTS
CCCCCCHHHHHHHHCCCEEEEEEECHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCH
FYTYALKRIKGSMYDYLRRIDWLPKDVRSLVKKYEDLIYECKDNEYIDDNSVAEKLHIEK
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
NDVDKIKFSLSKRQILQLDEYFLNNEEDTWLEDIQEENDPEILAYKDMLHDKLTESIEKL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHH
EEREKLILSLYYNEGLTFKEIGSVLEISESRVSQLHSVILVKLKKMIQGSE
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]