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The map label for this gene is appB [H]

Identifier: 160902780

GI number: 160902780

Start: 1430703

End: 1431638

Strand: Direct

Name: appB [H]

Synonym: Pmob_1334

Alternate gene names: 160902780

Gene position: 1430703-1431638 (Clockwise)

Preceding gene: 160902779

Following gene: 160902787

Centisome position: 65.94

GC content: 35.58

Gene sequence:

>936_bases
ATGTATTGGAGATATGCTTTTAAAAGGGTGTTAATGGGTGTTATGATTTATTTTGTTATTATTTTTGTGTATGCTGTACT
TTTCAACGTCATGTTTAAACTTCACTATAGCTACCTTTTGGAAGGACCAATATTACCCGAGATTTTCTCTGATTGGTTCG
ATACGATCACTTTTAATTATGGTCAATCTATGTATATAAGATCTTTTAAAGGAGAAACTGACGTCATTAAAATTATCTTA
GAAAGAGTACCTAATACCATGCTTCTTTTTACTATGGCTATTATCATAGATATTTTCTTGGGGGTGTATTTGGGCATAAA
AAAGGCTCAAAAAGCAGGGGGATTCATGGACAAAACAACCTCCATACTTACCATGATTTTTTACGGCCTTCCAACATGGT
GGGTTGGACTGGTAATGATCATGTTTTTTTCTTTCAAAATGCCTTTATTTCCTTCTGGTGGTATATTTAGCATCCCGCCT
CCTGTGGGGTTTATAAAAAGATTTGCAGATATTGTGTATCATTTAATTCTACCTTTAACTACGCTTGTTTTTTTTCGTTT
TTGGGGTAGAGCATATTTATCAAGAAATATCGTGTTAGCAAATTTGCAGAATGATTTCATAATATCCGCAAGGGCTAGGG
GCATTCCTGAAAGAAAAGTTTTGTTTGGGCACGCTTTAAGAGCTTCTGCTCCTCCAATCTTAACAATGTCGGTTTTATCT
GTGCTCGATTCTTTTTCTGGGGCATTGATCATCGAAGGTATTTTTAATTGGCCTGGAATGGGGAACCTTTTTTGGGCTGC
TATTCAACAAGATGATATTCCAGTTCTTTTGGGTAATTTGTCTTTTACAACTCTTTTATACATCTGCGGTATTGTAATTT
TAGACTTAATTTACGGGGTGCTTGACCCTAGAATAAAAGTTGGCGGGAAAGAGTGA

Upstream 100 bases:

>100_bases
AAGAGATAGATCAGGAAGTAGAAAAGGTTTGAAAAATCCCCACAAATGTGGGGTTTTTTAATTTTGATGAAAATAAATAG
ATATTAAGGTGGTTACGTCG

Downstream 100 bases:

>100_bases
ATAAAATCCCGCCAAGAGTTTAGTTTTGTCTTCCCCTATCCCCATTAGTATATGAAATGAATATCTTTTCGAAATTCACC
ATTTGCTTTTCTATGCTTAC

Product: binding-protein-dependent transport systems inner membrane component

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MYWRYAFKRVLMGVMIYFVIIFVYAVLFNVMFKLHYSYLLEGPILPEIFSDWFDTITFNYGQSMYIRSFKGETDVIKIIL
ERVPNTMLLFTMAIIIDIFLGVYLGIKKAQKAGGFMDKTTSILTMIFYGLPTWWVGLVMIMFFSFKMPLFPSGGIFSIPP
PVGFIKRFADIVYHLILPLTTLVFFRFWGRAYLSRNIVLANLQNDFIISARARGIPERKVLFGHALRASAPPILTMSVLS
VLDSFSGALIIEGIFNWPGMGNLFWAAIQQDDIPVLLGNLSFTTLLYICGIVILDLIYGVLDPRIKVGGKE

Sequences:

>Translated_311_residues
MYWRYAFKRVLMGVMIYFVIIFVYAVLFNVMFKLHYSYLLEGPILPEIFSDWFDTITFNYGQSMYIRSFKGETDVIKIIL
ERVPNTMLLFTMAIIIDIFLGVYLGIKKAQKAGGFMDKTTSILTMIFYGLPTWWVGLVMIMFFSFKMPLFPSGGIFSIPP
PVGFIKRFADIVYHLILPLTTLVFFRFWGRAYLSRNIVLANLQNDFIISARARGIPERKVLFGHALRASAPPILTMSVLS
VLDSFSGALIIEGIFNWPGMGNLFWAAIQQDDIPVLLGNLSFTTLLYICGIVILDLIYGVLDPRIKVGGKE
>Mature_311_residues
MYWRYAFKRVLMGVMIYFVIIFVYAVLFNVMFKLHYSYLLEGPILPEIFSDWFDTITFNYGQSMYIRSFKGETDVIKIIL
ERVPNTMLLFTMAIIIDIFLGVYLGIKKAQKAGGFMDKTTSILTMIFYGLPTWWVGLVMIMFFSFKMPLFPSGGIFSIPP
PVGFIKRFADIVYHLILPLTTLVFFRFWGRAYLSRNIVLANLQNDFIISARARGIPERKVLFGHALRASAPPILTMSVLS
VLDSFSGALIIEGIFNWPGMGNLFWAAIQQDDIPVLLGNLSFTTLLYICGIVILDLIYGVLDPRIKVGGKE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence, though, unlike OPP, is incapable of transportin

COG id: COG0601

COG function: function code EP; ABC-type dipeptide/oligopeptide/nickel transport systems, permease components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789965, Length=256, Percent_Identity=31.25, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1788504, Length=288, Percent_Identity=27.0833333333333, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787497, Length=278, Percent_Identity=26.2589928057554, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1789888, Length=321, Percent_Identity=24.9221183800623, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1787761, Length=243, Percent_Identity=30.4526748971193, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787053, Length=267, Percent_Identity=24.7191011235955, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1787550, Length=286, Percent_Identity=23.7762237762238, Blast_Score=72, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35469; Mature: 35469

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCCCCCCCCC
>Mature Secondary Structure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HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]