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The map label for this gene is rfbB [C]

Identifier: 160877570

GI number: 160877570

Start: 5279560

End: 5280567

Strand: Direct

Name: rfbB [C]

Synonym: Sbal195_4469

Alternate gene names: 160877570

Gene position: 5279560-5280567 (Clockwise)

Preceding gene: 160877569

Following gene: 160877573

Centisome position: 98.73

GC content: 45.73

Gene sequence:

>1008_bases
ATGAAATACTTAGTCACAGGCGCTGCGGGTTTTATCGGCGCCAAAGTCAGTGAACGCCTTTGCTTGCTTGGCCATGAAGT
TATCGGCATTGATAACCTCAATGACTATTACGACGTCAATCTCAAATTGGCACGTTTAGACCTTTTACAAACTTTAGATA
ATTTCCATTTTATTAAACTCGATCTCGCAGACAGAGAAGGTATTGCTGCGCTTTTTGCGCTGCATGCATTCCAACGTGTT
ATCCATCTTGCGGCTCAAGCAGGTGTGCGTTACTCGCTCGACAATCCGTTAGCCTATGCCGACAGTAATTTAATTGGCCA
TCTCACTATTTTAGAAGGATGTCGCCACCATAAAATTGAGCATCTCGTGTATGCTTCATCTTCGTCTGTATATGGATTAA
ATCAGAAAATGCCCTTCTCCACTGAAGACAGCATAGATCATCCCATTTCGCTCTATGCCGCCACGAAGAAAGCCAATGAA
TTGATGTCGCACACTTACTCACATTTATATCAATTACCGACGACAGGTCTGCGCTTTTTTACCGTCTACGGCCCTTGGGG
CCGTCCCGATATGGCTTTATTTAAATTCACCAAAGCCATACTCGCAGGTGAAGTGATCGATGTGTATAACCATGGAGACC
TCAGTCGAGACTTTACCTATATTGATGATATTGTTGAGGGAATCATTCTGGTACAAGCCAAACCACCTCGCCCAAATACG
GACTGGACAGTAGAAGCTGGCACGCCAGCCACCAGCAGCGCGCCATATCGCGTCTTTAATATTGGCAATGGTAGCCCAGT
GCAGTTACTCGACTTTATTACAGCGCTAGAAGATGCCTTAGGCATTAAAGCGAACAAGAACCTGCTACCGATGCAGCCGG
GCGATGTGCATTCGACATGGGCCGACACAAACGACCTATTTGATGCCGTTGGCTATAAGCCACTGGTGGATATCAACACT
GGCGTCATGCAATTTGTGGACTGGTATCGCCAGTTCTATAACAAATAA

Upstream 100 bases:

>100_bases
TCTCCAACCGTATGGTTGATGAACTCAATGATGTTGCGGATAAAGTCTATACCCGAGATCTATTTCACCATGATTAATAC
ACTTTAAATAAAGGTCATAA

Downstream 100 bases:

>100_bases
GTTAGCGCCCAAAAGACAAACAAAAAGGCCTGCAAGAATGCAGGCCTTTGCTTTAATCATGAATGTTAGAAGCGATACTC
AAAACTGCTAAAGAGCGTGG

Product: NAD-dependent epimerase/dehydratase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKYLVTGAAGFIGAKVSERLCLLGHEVIGIDNLNDYYDVNLKLARLDLLQTLDNFHFIKLDLADREGIAALFALHAFQRV
IHLAAQAGVRYSLDNPLAYADSNLIGHLTILEGCRHHKIEHLVYASSSSVYGLNQKMPFSTEDSIDHPISLYAATKKANE
LMSHTYSHLYQLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGEVIDVYNHGDLSRDFTYIDDIVEGIILVQAKPPRPNT
DWTVEAGTPATSSAPYRVFNIGNGSPVQLLDFITALEDALGIKANKNLLPMQPGDVHSTWADTNDLFDAVGYKPLVDINT
GVMQFVDWYRQFYNK

Sequences:

>Translated_335_residues
MKYLVTGAAGFIGAKVSERLCLLGHEVIGIDNLNDYYDVNLKLARLDLLQTLDNFHFIKLDLADREGIAALFALHAFQRV
IHLAAQAGVRYSLDNPLAYADSNLIGHLTILEGCRHHKIEHLVYASSSSVYGLNQKMPFSTEDSIDHPISLYAATKKANE
LMSHTYSHLYQLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGEVIDVYNHGDLSRDFTYIDDIVEGIILVQAKPPRPNT
DWTVEAGTPATSSAPYRVFNIGNGSPVQLLDFITALEDALGIKANKNLLPMQPGDVHSTWADTNDLFDAVGYKPLVDINT
GVMQFVDWYRQFYNK
>Mature_335_residues
MKYLVTGAAGFIGAKVSERLCLLGHEVIGIDNLNDYYDVNLKLARLDLLQTLDNFHFIKLDLADREGIAALFALHAFQRV
IHLAAQAGVRYSLDNPLAYADSNLIGHLTILEGCRHHKIEHLVYASSSSVYGLNQKMPFSTEDSIDHPISLYAATKKANE
LMSHTYSHLYQLPTTGLRFFTVYGPWGRPDMALFKFTKAILAGEVIDVYNHGDLSRDFTYIDDIVEGIILVQAKPPRPNT
DWTVEAGTPATSSAPYRVFNIGNGSPVQLLDFITALEDALGIKANKNLLPMQPGDVHSTWADTNDLFDAVGYKPLVDINT
GVMQFVDWYRQFYNK

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=353, Percent_Identity=26.0623229461756, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56237023, Length=355, Percent_Identity=25.3521126760563, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI56118217, Length=355, Percent_Identity=25.3521126760563, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI189083684, Length=355, Percent_Identity=25.3521126760563, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI42516563, Length=340, Percent_Identity=25, Blast_Score=92, Evalue=5e-19,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=24.5125348189415, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI48994969, Length=354, Percent_Identity=26.8361581920904, Blast_Score=104, Evalue=7e-24,
Organism=Escherichia coli, GI1786974, Length=324, Percent_Identity=25, Blast_Score=103, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=24.1477272727273, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=24.0112994350282, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17568069, Length=345, Percent_Identity=23.1884057971014, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17539532, Length=340, Percent_Identity=24.7058823529412, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6319493, Length=248, Percent_Identity=30.6451612903226, Blast_Score=84, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19923002, Length=350, Percent_Identity=27.7142857142857, Blast_Score=111, Evalue=7e-25,
Organism=Drosophila melanogaster, GI21356223, Length=343, Percent_Identity=24.7813411078717, Blast_Score=88, Evalue=8e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37386; Mature: 37386

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYLVTGAAGFIGAKVSERLCLLGHEVIGIDNLNDYYDVNLKLARLDLLQTLDNFHFIKL
CCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCEEEEE
DLADREGIAALFALHAFQRVIHLAAQAGVRYSLDNPLAYADSNLIGHLTILEGCRHHKIE
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEHHHHHHHHHCCEE
HLVYASSSSVYGLNQKMPFSTEDSIDHPISLYAATKKANELMSHTYSHLYQLPTTGLRFF
EEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGEVIDVYNHGDLSRDFTYIDDIVEGIILVQAKPPRPNT
EEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCC
DWTVEAGTPATSSAPYRVFNIGNGSPVQLLDFITALEDALGIKANKNLLPMQPGDVHSTW
CEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ADTNDLFDAVGYKPLVDINTGVMQFVDWYRQFYNK
CCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYLVTGAAGFIGAKVSERLCLLGHEVIGIDNLNDYYDVNLKLARLDLLQTLDNFHFIKL
CCEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEEHHHHHHHHHCCCEEEEE
DLADREGIAALFALHAFQRVIHLAAQAGVRYSLDNPLAYADSNLIGHLTILEGCRHHKIE
EECCCCCHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEECCCCCEEEHHHHHHHHHCCEE
HLVYASSSSVYGLNQKMPFSTEDSIDHPISLYAATKKANELMSHTYSHLYQLPTTGLRFF
EEEEECCCCEEECCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEE
TVYGPWGRPDMALFKFTKAILAGEVIDVYNHGDLSRDFTYIDDIVEGIILVQAKPPRPNT
EEECCCCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCEEEEEECCCCCCC
DWTVEAGTPATSSAPYRVFNIGNGSPVQLLDFITALEDALGIKANKNLLPMQPGDVHSTW
CEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
ADTNDLFDAVGYKPLVDINTGVMQFVDWYRQFYNK
CCCHHHHHHCCCCEEEECCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]