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The map label for this gene is rep [H]

Identifier: 160877210

GI number: 160877210

Start: 4864301

End: 4866358

Strand: Reverse

Name: rep [H]

Synonym: Sbal195_4106

Alternate gene names: 160877210

Gene position: 4866358-4864301 (Counterclockwise)

Preceding gene: 160877214

Following gene: 160877197

Centisome position: 91.01

GC content: 48.2

Gene sequence:

>2058_bases
ATGATCCCATCCCTTTCTGTCGACTTGTTTGGATATTGCGCAAAGATGAAACTCAATCCCGCCCAAAACGATGCTGTTCA
TTATGTTTCAGGCCCTTGCCTAGTATTGGCGGGTGCGGGCAGTGGTAAGACGCGCGTAATTATCAATAAAATCGGTTACT
TGGTCGAAAAGTGCGGCTATAAGGCACGCAATATTGCGGCGGTGACCTTCACCAACAAGGCTGCCCGTGAGATGAAAGAG
CGGGTCGCTCAGTCCATGGGACGCAAAGAGGCCAGAGGTCTGTGGATTTCAACCTTCCACACCTTAGGGCTTGAAATCAT
TAAGCGTGAGCATAAGGTCTTAGGCTTAAAGCCGGGATTTTCGCTGTTTGATGATCAAGATACCTTGGCACTGCTCAAAG
AGTTAACCCAAGACGAGTTAGACGAAGATAAAGATTTACTCAAAGCGCTCGCCAGTACGATTTCAAACTGGAAGGGCGGT
TTAATTATTCCCGAGCATGCACGCAAGATCGCTAAAGATGAGCAGCAGCATTTATTTGCCATGTTGTATCAGCGTTATGC
CCAGCACATGAAAGCCTATAACGCCTTAGATTTTGACGATTTAATCCTGATGCCAACCTTATTGTTGCGGCATCATGAAG
AAGTGCGTACCCGCTGGCAAACCAAGATCCAATACTTGCTGGTGGATGAATATCAAGATACCAATACCAGCCAATATGAG
ATGGTAAAGCTGTTAGTGGGTGATCGGGCGCGCTTTACCGTAGTGGGTGACGACGATCAGTCGATCTATTCATGGCGCGG
TGCTAAACCACAAAACTTAGTGTTATTGGGTACGGATTTTCCGCAGCTTAAGTTAATTAAGCTCGAGCAGAATTACCGTT
CTAGTCAACGGATCCTGCGGGCTGCTAACATTCTTATCGCGAACAATCCCCATGTTTACGATAAAGCGCTCTTTAGTGAG
CTTGCCTATGGGGAGCCGCTGCGGGTGCTATTTGCCGCCAACGAGGAGCAAGAGGCTGAACGTGTGGTCGCGGAAATAGT
GCGACACAAGTTTGTTGGCCGTACTCAGTTTGGCGACTATGCGATTCTGTATCGCGGCAACCATCAATCGCGGTTACTCG
AGCGCGCGCTGATGACGAACCGCATTCCTTACAAATTGAGTGGTGGCACGTCATTCTTCGCCCGTGCTGAAATCAAAGAC
ATCATGGGCTATCTAAAGCTGGTGGTGAATCCCGATGATGATAATGCTTTCCTGCGCATAGTTAACTTGCCTAAACGTGG
CATAGGCCCATCGACTTTAGAGCGCTTAGGTAATTTCGCTAATGAGAAGCATATTTCTATGTTTGCGGCGATTTTTGAGC
CTGAACTCAATCATCATTTACCGCCGGCGGCTATGTCATCCCTGTATCAATTCGGTCGTTTCATTGTCGAGACAGGGGAT
CATGCAGAGCGTGGTGAACCTGTCGAGGCTGTGAAGCATTTGATCCGTAAGATCAATTATGAAGATTATCTATACGAAAC
CAGCACGAGTGCCAAAGCCGCTGAAATGCGGATGAAGAATATCTCAGAGCTATACCGTTGGGTAACTGAGATGCTCGCGG
GTGATGAATTAGATGAGCCAATGACCTTGCCTGAGGTTGTGACTCGACTGACGCTCAGAGACATGATGGAGCGCAATAGC
GAGGACGAAGGCGGCGATCAAGTGCAGTTGATGACCTTGCATGCTTCTAAGGGCTTAGAGTTCCCGTTTGTGTTTATGGT
GGGAGTGGAGGAGCGGATCTTACCCCATCAGACCAGTATAGATGAAGACAATGTCGATGAAGAGCGCCGCTTAGCCTACG
TGGGCATCACTCGGGCTCAACGTGAACTGTGGTTTACCCTCTGTCGTGAAAGACGTCAATTTGGTGAAACCATGCGTTGT
GAGCCGAGCCGTTTCTTGCTTGAGTTACCTCAAGATGATGTGGTGTGGGAAAATCGTAAACCACAGCAGACAGAACAGGA
GCGGATGCAGACTGGGCGAGCCAACATTTCTAGCTTAAGGGATATGTTTAAAAAATAA

Upstream 100 bases:

>100_bases
GTCGTTGTTGAGGCTTAAATCACAGCCAAGCATGTCTATTGGGTTGAGTTCTGCTCAGCCCAAGCGGGGATTTGTTAGCC
TTAAAACCGCCGATTCTGCT

Downstream 100 bases:

>100_bases
CGAGCGTTTCGCTTACTGTTATTTAAAGTCAATCGCTCGGGTTTTATCTAGGCATATTCGAGCTGAGTACAGATCTGTGA
TTGGGTGTCACTTAAGGTAA

Product: ATP-dependent DNA helicase Rep

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 685; Mature: 685

Protein sequence:

>685_residues
MIPSLSVDLFGYCAKMKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKE
RVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGG
LIIPEHARKIAKDEQQHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYE
MVKLLVGDRARFTVVGDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSE
LAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKD
IMGYLKLVVNPDDDNAFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGD
HAERGEPVEAVKHLIRKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNS
EDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRC
EPSRFLLELPQDDVVWENRKPQQTEQERMQTGRANISSLRDMFKK

Sequences:

>Translated_685_residues
MIPSLSVDLFGYCAKMKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKE
RVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGG
LIIPEHARKIAKDEQQHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYE
MVKLLVGDRARFTVVGDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSE
LAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKD
IMGYLKLVVNPDDDNAFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGD
HAERGEPVEAVKHLIRKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNS
EDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRC
EPSRFLLELPQDDVVWENRKPQQTEQERMQTGRANISSLRDMFKK
>Mature_685_residues
MIPSLSVDLFGYCAKMKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGYKARNIAAVTFTNKAAREMKE
RVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGFSLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGG
LIIPEHARKIAKDEQQHLFAMLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYE
MVKLLVGDRARFTVVGDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILRAANILIANNPHVYDKALFSE
LAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDYAILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKD
IMGYLKLVVNPDDDNAFLRIVNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGD
HAERGEPVEAVKHLIRKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEPMTLPEVVTRLTLRDMMERNS
EDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSIDEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRC
EPSRFLLELPQDDVVWENRKPQQTEQERMQTGRANISSLRDMFKK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=668, Percent_Identity=67.5149700598802, Blast_Score=915, Evalue=0.0,
Organism=Escherichia coli, GI2367296, Length=645, Percent_Identity=38.9147286821705, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=357, Percent_Identity=29.1316526610644, Blast_Score=105, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6322369, Length=447, Percent_Identity=32.4384787472036, Blast_Score=197, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78777; Mature: 78777

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPSLSVDLFGYCAKMKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGY
CCCCCCHHHHHHHHHEECCCCCCCCEEEECCCEEEEEECCCCCCEEHHHHHHHHHHHHCC
KARNIAAVTFTNKAAREMKERVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGF
CCCCEEEEEECCHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCC
SLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQQHLFA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
MLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHH
MVKLLVGDRARFTVVGDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILR
HHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHH
AANILIANNPHVYDKALFSELAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDY
HHCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCE
AILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRI
EEEEECCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEE
VNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGD
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCC
HAERGEPVEAVKHLIRKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEP
CCCCCCHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSI
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCC
DEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVVWENRK
CCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEECCCC
PQQTEQERMQTGRANISSLRDMFKK
CCHHHHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MIPSLSVDLFGYCAKMKLNPAQNDAVHYVSGPCLVLAGAGSGKTRVIINKIGYLVEKCGY
CCCCCCHHHHHHHHHEECCCCCCCCEEEECCCEEEEEECCCCCCEEHHHHHHHHHHHHCC
KARNIAAVTFTNKAAREMKERVAQSMGRKEARGLWISTFHTLGLEIIKREHKVLGLKPGF
CCCCEEEEEECCHHHHHHHHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHEEECCCCC
SLFDDQDTLALLKELTQDELDEDKDLLKALASTISNWKGGLIIPEHARKIAKDEQQHLFA
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHH
MLYQRYAQHMKAYNALDFDDLILMPTLLLRHHEEVRTRWQTKIQYLLVDEYQDTNTSQYE
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCHHH
MVKLLVGDRARFTVVGDDDQSIYSWRGAKPQNLVLLGTDFPQLKLIKLEQNYRSSQRILR
HHHHHHCCCEEEEEECCCCCHHHHCCCCCCCCEEEEECCCCCEEEEEEHHHHHHHHHHHH
AANILIANNPHVYDKALFSELAYGEPLRVLFAANEEQEAERVVAEIVRHKFVGRTQFGDY
HHCEEEECCCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCE
AILYRGNHQSRLLERALMTNRIPYKLSGGTSFFARAEIKDIMGYLKLVVNPDDDNAFLRI
EEEEECCHHHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEECCCCCCEEEEE
VNLPKRGIGPSTLERLGNFANEKHISMFAAIFEPELNHHLPPAAMSSLYQFGRFIVETGD
EECCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHEECCC
HAERGEPVEAVKHLIRKINYEDYLYETSTSAKAAEMRMKNISELYRWVTEMLAGDELDEP
CCCCCCHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
MTLPEVVTRLTLRDMMERNSEDEGGDQVQLMTLHASKGLEFPFVFMVGVEERILPHQTSI
CCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCEEEEECCHHHHCCCCCCC
DEDNVDEERRLAYVGITRAQRELWFTLCRERRQFGETMRCEPSRFLLELPQDDVVWENRK
CCCCCCHHHEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHEECCCCCCEECCCC
PQQTEQERMQTGRANISSLRDMFKK
CCHHHHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]