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The map label for this gene is rihA [H]

Identifier: 160876874

GI number: 160876874

Start: 4444107

End: 4445063

Strand: Direct

Name: rihA [H]

Synonym: Sbal195_3770

Alternate gene names: 160876874

Gene position: 4444107-4445063 (Clockwise)

Preceding gene: 160876870

Following gene: 160876875

Centisome position: 83.11

GC content: 51.93

Gene sequence:

>957_bases
ATGACTCGTCACATCATTCTTGATTGCGATCCCGGCCATGACGATGCTATCGCGCTGATCTTAGCCTTAGCTCACCCAGA
CCTCGTTCCCCTTGCCGTCACGACTAGCGCAGGCAATCAAACCCCAGATAAAACCCTCAACAACGCCCTGCGGATCTTAA
CCCTGTTAAACCGTGGTGATATCCCAGTCGCGGGCGGTGCCACTAAGCCGCTAACACGTCAACTGATTATCGCCGACAAT
GTTCACGGCGAGACAGGACTCGATGGCCCCGCATTGCCTGAACCTAGCTTCAGCCCACAAGCCATAACGGCCGTTGAACT
GATGGCGCAGCAAATCCGCCAAAGCACTCAGCCAGTGACTTTAGTGCCCACTGGGCCGCTGACGAACATTGCTCTTTTAC
TGGCGAGTCATAGCGAGCTACACCCTAAAATAGAGCGCATTGTGCTGATGGGCGGCGCCGCGGGTGTCGGTAATTGGACG
CCTGCCGCCGAGTTTAATATTTTTGTCGATCCCGAAGCCGCCGATATCGTCTTTAAGTCAGGTATTCCCATCACTATGTG
CGGGCTCGATGTGACCCATCAAGCGCAAATCATGGATGCAGACATAGAACGGATTCGCGCCATCCCAAATCCAATTGCTC
AGTGCGTCGCCGAGTTATTGGATTTCTTTATGATTTACCACAGAGATCCTAAATGGGGCTTTATTGGCGCGCCATTGCAC
GACCCCTGTACTATCGCTTGGTTGCTCAAACCTGAGCTGTTTGAGGCACAAGATTGCTGGGTCGGGATTGAAACCCAAAG
CGAGTTGACCTTAGGCATGACAGTGGTTGATCGCTATCAACTCACTGGCAAAACCGCCAATGCGACTGTGCTATTTGGGC
TCGATAGACTAGGCTTTGTCGATTTACTGGTAGACAGTTTACGTGTCTATGATCCAACTTATCTCAATCGCAGATGA

Upstream 100 bases:

>100_bases
ACGCTCACGGTTTGCTCTGCAAGTACCCTCCACAGGCTAGCGCCTAACTTATGGCATGCTATTCTAAGCATCCAGTACCG
CTGTTTAAAGGAGTAACAGT

Downstream 100 bases:

>100_bases
AAATAACAGTGAGTTTTCTATGAGCCAGATACTCGTTATCGGCAGTGCAAATGCCGATCATGTGATGAAATTTGAGTACC
TGCCCGCAGCAGGGCAAACC

Product: ribonucleoside hydrolase 1

Products: uracil; ribose; cytosine [C]

Alternate protein names: Cytidine/uridine-specific hydrolase [H]

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MTRHIILDCDPGHDDAIALILALAHPDLVPLAVTTSAGNQTPDKTLNNALRILTLLNRGDIPVAGGATKPLTRQLIIADN
VHGETGLDGPALPEPSFSPQAITAVELMAQQIRQSTQPVTLVPTGPLTNIALLLASHSELHPKIERIVLMGGAAGVGNWT
PAAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDADIERIRAIPNPIAQCVAELLDFFMIYHRDPKWGFIGAPLH
DPCTIAWLLKPELFEAQDCWVGIETQSELTLGMTVVDRYQLTGKTANATVLFGLDRLGFVDLLVDSLRVYDPTYLNRR

Sequences:

>Translated_318_residues
MTRHIILDCDPGHDDAIALILALAHPDLVPLAVTTSAGNQTPDKTLNNALRILTLLNRGDIPVAGGATKPLTRQLIIADN
VHGETGLDGPALPEPSFSPQAITAVELMAQQIRQSTQPVTLVPTGPLTNIALLLASHSELHPKIERIVLMGGAAGVGNWT
PAAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDADIERIRAIPNPIAQCVAELLDFFMIYHRDPKWGFIGAPLH
DPCTIAWLLKPELFEAQDCWVGIETQSELTLGMTVVDRYQLTGKTANATVLFGLDRLGFVDLLVDSLRVYDPTYLNRR
>Mature_317_residues
TRHIILDCDPGHDDAIALILALAHPDLVPLAVTTSAGNQTPDKTLNNALRILTLLNRGDIPVAGGATKPLTRQLIIADNV
HGETGLDGPALPEPSFSPQAITAVELMAQQIRQSTQPVTLVPTGPLTNIALLLASHSELHPKIERIVLMGGAAGVGNWTP
AAEFNIFVDPEAADIVFKSGIPITMCGLDVTHQAQIMDADIERIRAIPNPIAQCVAELLDFFMIYHRDPKWGFIGAPLHD
PCTIAWLLKPELFEAQDCWVGIETQSELTLGMTVVDRYQLTGKTANATVLFGLDRLGFVDLLVDSLRVYDPTYLNRR

Specific function: Hydrolyzes cytidine or uridine to ribose and cytosine or uracil, respectively [H]

COG id: COG1957

COG function: function code F; Inosine-uridine nucleoside N-ribohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IUNH family. RihA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786871, Length=310, Percent_Identity=71.2903225806452, Blast_Score=463, Evalue=1e-132,
Organism=Escherichia coli, GI1788486, Length=312, Percent_Identity=41.9871794871795, Blast_Score=245, Evalue=3e-66,
Organism=Escherichia coli, GI1786213, Length=303, Percent_Identity=44.2244224422442, Blast_Score=208, Evalue=5e-55,
Organism=Caenorhabditis elegans, GI32565474, Length=190, Percent_Identity=32.6315789473684, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17565698, Length=212, Percent_Identity=32.0754716981132, Blast_Score=83, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320608, Length=257, Percent_Identity=35.408560311284, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24641839, Length=193, Percent_Identity=35.2331606217617, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24583915, Length=189, Percent_Identity=35.4497354497354, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24641837, Length=197, Percent_Identity=36.0406091370558, Blast_Score=75, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015910
- InterPro:   IPR023186
- InterPro:   IPR001910
- InterPro:   IPR022975 [H]

Pfam domain/function: PF01156 IU_nuc_hydro [H]

EC number: NA

Molecular weight: Translated: 34509; Mature: 34378

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: PS01247 IUNH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRHIILDCDPGHDDAIALILALAHPDLVPLAVTTSAGNQTPDKTLNNALRILTLLNRGD
CCCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IPVAGGATKPLTRQLIIADNVHGETGLDGPALPEPSFSPQAITAVELMAQQIRQSTQPVT
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LVPTGPLTNIALLLASHSELHPKIERIVLMGGAAGVGNWTPAAEFNIFVDPEAADIVFKS
EEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEC
GIPITMCGLDVTHQAQIMDADIERIRAIPNPIAQCVAELLDFFMIYHRDPKWGFIGAPLH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCEECCCCC
DPCTIAWLLKPELFEAQDCWVGIETQSELTLGMTVVDRYQLTGKTANATVLFGLDRLGFV
CCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCHHHHH
DLLVDSLRVYDPTYLNRR
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TRHIILDCDPGHDDAIALILALAHPDLVPLAVTTSAGNQTPDKTLNNALRILTLLNRGD
CCEEEEECCCCCCHHHHHHHHHCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHCCCC
IPVAGGATKPLTRQLIIADNVHGETGLDGPALPEPSFSPQAITAVELMAQQIRQSTQPVT
CCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEE
LVPTGPLTNIALLLASHSELHPKIERIVLMGGAAGVGNWTPAAEFNIFVDPEAADIVFKS
EEECCCHHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCCCCEEEEEC
GIPITMCGLDVTHQAQIMDADIERIRAIPNPIAQCVAELLDFFMIYHRDPKWGFIGAPLH
CCCEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHEECCCCCCEECCCCC
DPCTIAWLLKPELFEAQDCWVGIETQSELTLGMTVVDRYQLTGKTANATVLFGLDRLGFV
CCEEEEEEECCCCCCCCCCEEEECCCCCEEEEEEEEEEEEECCCCCCEEEEEECCHHHHH
DLLVDSLRVYDPTYLNRR
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uridine; H2O; cytidine [C]

Specific reaction: uridine + H2O = uracil + ribose cytidine + H2O = cytosine + ribose [C]

General reaction: Pyrimidine-specific nucleoside hydrolase [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA