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The map label for this gene is amtB [H]

Identifier: 160876832

GI number: 160876832

Start: 4394176

End: 4395489

Strand: Direct

Name: amtB [H]

Synonym: Sbal195_3728

Alternate gene names: 160876832

Gene position: 4394176-4395489 (Clockwise)

Preceding gene: 160876831

Following gene: 160876839

Centisome position: 82.18

GC content: 50.23

Gene sequence:

>1314_bases
ATGATGCTAGCGAACTTCAGACTCATTTTTAAATATCTATTGATATTATTCAGTATGTTGGCGACCAATGCTGTCGCCGA
TGAGAGTCAGTTCAGCGGCGCAAATACCGCGTGGATCTTAAGCTCATCCGCGCTGGTGTTGTTGATGACCTTACCCGGAT
TAGCCTTGTTTTATGGCGGCTTAGTGCGCAGCAAGAACGTGCTCTCGATCTTGATGCAATGCTTCTCGATCGCCGCCCTT
GTCTCAGTTTTATGGTTTGCCATAGGTTATTCACTCGCCTTCGATACAGGTAATGGATTTATCGGTGGCTTAGGTAAAGC
ATTACTGTCGGGCATTGGCCGAGATAGTCTCAGCGGGGATATTCCTGAGCCCCTGTTTATGCTATTTCAAATGACCTTTG
CCATTATAACGCCAGCATTGATCATCGGTGGCTTTGCCGAGCGAATGAAATTTTCGGCCGTGCTCATCTTTTCTGGAGCT
TGGTTATTATTGGTTTATGCGCCGATCACCCACTGGGTGTGGGGCGGTGGCTGGTTAGCACAGCTAGGACTGTACGATTT
CGCGGGCGGAACCGTGGTGCATATTACCGCCGGGGTTGCAGCGCTCGTGGCGGCTAAAGTGCTAGGTCCGCGCAAGGGAT
TTTTAAACTCAGCGATTATGCCGCACAACCTCACCATGACAGTCACAGGTGCGGGCATGCTGTGGGTCGGTTGGTTCGGT
TTTAATGGTGGTAGCGCGCTTGGCGCCAATGGCACAGCTGCCATGGCGATTCTAGCAACTCATCTCGCCGCTTCTATGGG
CGCGATGACTTGGGCAGCAATTGAGTGGATTAAATTTGGCAAACCCAGCGCCCTTGGCATAGTCACAGGCATGGTAGCAG
GCTTAGGCACTATTACTCCGGCGTCTGGTTATGTTGGCCCTGCAGGTGCCTTAGTGATTGGTTTACTCGGCGGGATAGTG
TGTTTTTACTCAACGGTCTATATCAAACAGAAACTAAAGATCGATGACTCCTTAGACGTTTTCCCCGTGCACGGGGTCGG
CGGCATTCTCGGAACCCTTTTAGCCGGCGTGTTTAGCTCGACCCAATTAGGCGTCTTCAGCGGCTACGGTTTTGCGGCCG
TGAACGAAACCATGATAGACCAACTTGGGGTACAGATTATTGGGGTTGCAGCAACATTCACTTATACAGCTGTGGTCACT
TGGTTACTGTTCGTGATCATCCGTAAACTAGTTGGCGGCCTTCGTGTAAGCACAGAACAAGAAGTTAACGGCTTAGATTT
ATCGGAACACGAAGAAACTGGCTATAGTCTTTAA

Upstream 100 bases:

>100_bases
CCGCTGCCCATACGGGTAAAGTGGGCGATGGCAAAATCTTTGTGACCGAACTCCAACAAGTCGTGCGCATTCGCACTTCG
GAAGAAGGAGTGGATGCCCT

Downstream 100 bases:

>100_bases
ACGCTTTAGGCTACAACTTAAGCTATAACGCTTGAACAGCAATAAGGGCACCTAGGTGCCCTTATTGCTGACTGATTTTA
TGATGCTAACGTTTAAAATG

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MMLANFRLIFKYLLILFSMLATNAVADESQFSGANTAWILSSSALVLLMTLPGLALFYGGLVRSKNVLSILMQCFSIAAL
VSVLWFAIGYSLAFDTGNGFIGGLGKALLSGIGRDSLSGDIPEPLFMLFQMTFAIITPALIIGGFAERMKFSAVLIFSGA
WLLLVYAPITHWVWGGGWLAQLGLYDFAGGTVVHITAGVAALVAAKVLGPRKGFLNSAIMPHNLTMTVTGAGMLWVGWFG
FNGGSALGANGTAAMAILATHLAASMGAMTWAAIEWIKFGKPSALGIVTGMVAGLGTITPASGYVGPAGALVIGLLGGIV
CFYSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGVFSGYGFAAVNETMIDQLGVQIIGVAATFTYTAVVT
WLLFVIIRKLVGGLRVSTEQEVNGLDLSEHEETGYSL

Sequences:

>Translated_437_residues
MMLANFRLIFKYLLILFSMLATNAVADESQFSGANTAWILSSSALVLLMTLPGLALFYGGLVRSKNVLSILMQCFSIAAL
VSVLWFAIGYSLAFDTGNGFIGGLGKALLSGIGRDSLSGDIPEPLFMLFQMTFAIITPALIIGGFAERMKFSAVLIFSGA
WLLLVYAPITHWVWGGGWLAQLGLYDFAGGTVVHITAGVAALVAAKVLGPRKGFLNSAIMPHNLTMTVTGAGMLWVGWFG
FNGGSALGANGTAAMAILATHLAASMGAMTWAAIEWIKFGKPSALGIVTGMVAGLGTITPASGYVGPAGALVIGLLGGIV
CFYSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGVFSGYGFAAVNETMIDQLGVQIIGVAATFTYTAVVT
WLLFVIIRKLVGGLRVSTEQEVNGLDLSEHEETGYSL
>Mature_437_residues
MMLANFRLIFKYLLILFSMLATNAVADESQFSGANTAWILSSSALVLLMTLPGLALFYGGLVRSKNVLSILMQCFSIAAL
VSVLWFAIGYSLAFDTGNGFIGGLGKALLSGIGRDSLSGDIPEPLFMLFQMTFAIITPALIIGGFAERMKFSAVLIFSGA
WLLLVYAPITHWVWGGGWLAQLGLYDFAGGTVVHITAGVAALVAAKVLGPRKGFLNSAIMPHNLTMTVTGAGMLWVGWFG
FNGGSALGANGTAAMAILATHLAASMGAMTWAAIEWIKFGKPSALGIVTGMVAGLGTITPASGYVGPAGALVIGLLGGIV
CFYSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSSTQLGVFSGYGFAAVNETMIDQLGVQIIGVAATFTYTAVVT
WLLFVIIRKLVGGLRVSTEQEVNGLDLSEHEETGYSL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=418, Percent_Identity=47.3684210526316, Blast_Score=344, Evalue=6e-96,
Organism=Caenorhabditis elegans, GI17550182, Length=440, Percent_Identity=30.4545454545455, Blast_Score=154, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=439, Percent_Identity=29.6127562642369, Blast_Score=147, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI32564042, Length=414, Percent_Identity=27.2946859903382, Blast_Score=124, Evalue=9e-29,
Organism=Caenorhabditis elegans, GI133931100, Length=436, Percent_Identity=26.605504587156, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6324187, Length=424, Percent_Identity=37.9716981132075, Blast_Score=248, Evalue=2e-66,
Organism=Saccharomyces cerevisiae, GI6325396, Length=418, Percent_Identity=35.8851674641148, Blast_Score=224, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=32.7751196172249, Blast_Score=202, Evalue=6e-53,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=30.2600472813239, Blast_Score=129, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 45770; Mature: 45770

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMLANFRLIFKYLLILFSMLATNAVADESQFSGANTAWILSSSALVLLMTLPGLALFYGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LVRSKNVLSILMQCFSIAALVSVLWFAIGYSLAFDTGNGFIGGLGKALLSGIGRDSLSGD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCCCC
IPEPLFMLFQMTFAIITPALIIGGFAERMKFSAVLIFSGAWLLLVYAPITHWVWGGGWLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCHHHH
QLGLYDFAGGTVVHITAGVAALVAAKVLGPRKGFLNSAIMPHNLTMTVTGAGMLWVGWFG
HHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCEEEEEEEE
FNGGSALGANGTAAMAILATHLAASMGAMTWAAIEWIKFGKPSALGIVTGMVAGLGTITP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
ASGYVGPAGALVIGLLGGIVCFYSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCC
TQLGVFSGYGFAAVNETMIDQLGVQIIGVAATFTYTAVVTWLLFVIIRKLVGGLRVSTEQ
CCCCEECCCCHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVNGLDLSEHEETGYSL
CCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMLANFRLIFKYLLILFSMLATNAVADESQFSGANTAWILSSSALVLLMTLPGLALFYGG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
LVRSKNVLSILMQCFSIAALVSVLWFAIGYSLAFDTGNGFIGGLGKALLSGIGRDSLSGD
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCCCCCCC
IPEPLFMLFQMTFAIITPALIIGGFAERMKFSAVLIFSGAWLLLVYAPITHWVWGGGWLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHHHHHHHHHHHHCCCHHHH
QLGLYDFAGGTVVHITAGVAALVAAKVLGPRKGFLNSAIMPHNLTMTVTGAGMLWVGWFG
HHHHHHCCCCEEEEEHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCEEEEEEEE
FNGGSALGANGTAAMAILATHLAASMGAMTWAAIEWIKFGKPSALGIVTGMVAGLGTITP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
ASGYVGPAGALVIGLLGGIVCFYSTVYIKQKLKIDDSLDVFPVHGVGGILGTLLAGVFSS
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHHHHHHHHCC
TQLGVFSGYGFAAVNETMIDQLGVQIIGVAATFTYTAVVTWLLFVIIRKLVGGLRVSTEQ
CCCCEECCCCHHHHHHHHHHHHCHHEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EVNGLDLSEHEETGYSL
CCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]