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The map label for this gene is carA [H]

Identifier: 160876632

GI number: 160876632

Start: 4145814

End: 4146974

Strand: Reverse

Name: carA [H]

Synonym: Sbal195_3526

Alternate gene names: 160876632

Gene position: 4146974-4145814 (Counterclockwise)

Preceding gene: 160876633

Following gene: 160876631

Centisome position: 77.55

GC content: 48.23

Gene sequence:

>1161_bases
ATGGAGGTCGCGTTGACAAAGTCTGCCTTACTCGTACTCGAAGATGGAACTGTATTCTCTGGCACAGCAATTGGTGCCGA
TGGTCTCTCTGTTGGTGAAGTTGTTTTTAACACTTCCATGACAGGTTACCAAGAAATTTTAACTGATCCATCCTACTCAC
GCCAGATTGTAACATTAACCTACCCACACATAGGCAACACTGGTACCAATGATGAAGATGTTGAATCCGATGGGGTTCAC
GCTTGTGGTCTTATCATTCGTGATCTTCCGCTCATCGCAAGTAGTTTCCGCAATCAACAAAGCTTAAGTGATTATCTCAA
AGCGAACAATGTCGTTGGCATAGCAGATATCGATACCCGCAAGTTAACTCGTATTCTCCGTGAAAAAGGTGCACAAGCCG
GTTGTATTATGGCAGGTGCAGATTTAGACGAAGCAAAAGCGCTCGCCGCTGCCAAAGCCTTCCCCGGTTTAAAAGGCATG
GATCTCGCTAAAGAAGTCACAACTAAAGCGGCCTATCCATGGCGCAAAGGTAGCTGGCGTTTAGTGGGCGGTTTACCTGC
AGATACGCCAGAAAATGAGCTTAAATACAAAGTAGTAGCCTACGACTACGGCGTTAAGCAAAACATTCTGCGTATGTTAG
TGGACCGCGGTTGCGACGTCACTGTTGTGCCAGCTAAAACCTCTGCCGCCGACGTGCTGGCGATGAATCCCGATGGTGTG
TTCCTTTCGAACGGCCCTGGCGATCCAGAACCATGCGATTACGCAATTGAAGCGATTCAACAGATCCTGAAAACCGACAC
ACCGATTTTCGGTATCTGTTTAGGTCATCAGTTGTTGGCTCTGGCCTCAGGTGCAAAAACCTTAAAGATGAAGTTTGGTC
ACCATGGTGCAAACCACCCAGTGAGCAACATCGAGAAAGGTAACGTGATGATCACCAGCCAAAACCACGGTTTTGCTGCC
GATGAAAGCACGCTACCCGCCAACATTAAGGTGACGCATAAGTCGCTGTTTGATGGTTCACTGCAAGGTATCCATCTTAC
GGACAAACCTGCGTTTAGCTTCCAAGGTCACCCTGAAGCGAGCCCAGGCCCGAACGATGCCGCACCTCTGTTCGATCACT
TTATCGAGCTTATTGAACAATACCGTCAGAACGCCAAGTAG

Upstream 100 bases:

>100_bases
TCCTGTAAACTCGCTGGAATTTGTCAAAATTTCGTAAATTAGCGGAAATTGGTATCTTAACATAACATAAAAACATTATA
TTTCAGTGGCTTGGCTCTTT

Downstream 100 bases:

>100_bases
TACGCTAGCTAGGAGAAGAGAGAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGCGCCGGCCCAAT
CGTGATTGGTCAGGCATGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPENELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK

Sequences:

>Translated_386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPENELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK
>Mature_386_residues
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNDEDVESDGVH
ACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTKAAYPWRKGSWRLVGGLPADTPENELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=38.6422976501305, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=37.2093023255814, Blast_Score=228, Evalue=5e-60,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=37.2093023255814, Blast_Score=228, Evalue=5e-60,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=77.7486910994764, Blast_Score=626, Evalue=0.0,
Organism=Caenorhabditis elegans, GI193204318, Length=388, Percent_Identity=37.1134020618557, Blast_Score=233, Evalue=2e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=397, Percent_Identity=40.3022670025189, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6322331, Length=406, Percent_Identity=36.2068965517241, Blast_Score=240, Evalue=3e-64,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=39.8457583547558, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=39.8457583547558, Blast_Score=236, Evalue=1e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41304; Mature: 41304

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEECCEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNDEDVESDGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTKAAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
LVGGLPADTPENELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
EEECCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEKGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIEQYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVALTKSALLVLEDGTVFSGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEECCEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNDEDVESDGVHACGLIIRDLPLIASSFRNQQSLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTKAAYPWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCEE
LVGGLPADTPENELKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAADVLAMNPDGV
EEECCCCCCCCCCCEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEKGNVMITSQNHGFAADESTLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIEQYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]