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The map label for this gene is truB [H]

Identifier: 160876521

GI number: 160876521

Start: 4031127

End: 4032092

Strand: Reverse

Name: truB [H]

Synonym: Sbal195_3415

Alternate gene names: 160876521

Gene position: 4032092-4031127 (Counterclockwise)

Preceding gene: 160876522

Following gene: 160876520

Centisome position: 75.4

GC content: 48.34

Gene sequence:

>966_bases
ATGGCTCGTCGTCCAAAAGGTCGTTTTATTGATGGGATAGTGCTGCTGGATAAATCCACTGGCATGAGTTCTAACTTTGC
ATTGCAAAGGGTAAAGCGCTTTTTTAATGCGAACAAAGCAGGGCATACGGGCGCACTCGATCCGTTAGCGACAGGTATGT
TACCTGTGTGCTTAGGCGAAGGGACTAAGTTTTCCCAGCATCTGTTAGATGCCGATAAACGTTATTTGGTCACCGCAAAG
CTGGGCGAGCGAACGGATACCAGTGATTCCGATGGCGAAGTGGTGCAAACCCGCGCCATTGATTTTACTGAGGCGCAGTT
ATTAACCGCGCTTGATTTCTTCCGTGGTGAGACTCAGCAAGTGCCTTCTATGTATTCCGCGCTTAAATATCAGGGCCAGC
CCCTGTATAAATATGCCCGTGAAGGCATTGAAGTGCCGCGCGAATCTCGCCCTATTACGGTATTTGAGCTTAATTTTATC
GGTCTTGAAGGCGATGAGCTGACCTTAGACATTCATTGTTCTAAGGGCACTTATATTCGCACTATCATAGACGATCTCGG
TGAAATGCTCGGTTGCGGCGCCCATGTCATCATGTTGCGTCGCACTCAAGTTGCACAATATCCCTATGCAAGAATGGTTT
CCTTAGAACAACTTGAAGCGCTTGTTGCTCAAGCACATGAACAACAGATAGATCCGAGCGTCTTACTTGATCCGCTGTTA
TTACCTATGGATACGGCTGTGGCTGATTTCCCTGAGGTGAATGTGCCAGATGCAATCGCCCCTTACTTAATGCAAGGTCA
GGCTGTTCGGGTCCCCGTTAATGCAGACTTGAAAACCGATGAACTGGTGCGTATCACTTTAGGTGACATTCGCCGATTTG
TGGGGATTGGCACTATGAACGAAGACGGCCTACTCGCGCCGAAACGACTCATAGTGATCCATGACGAGCCTGCAGAAACT
GATTAG

Upstream 100 bases:

>100_bases
CGAAGCAGCAGCAATTTGGTAGCGAAGAGGCATCAGTTGAAGATGAAGTCTTAGGTGATGATGTTGCCGATGATGCAGAT
GAAACGGAAGGTAAAGATTA

Downstream 100 bases:

>100_bases
CCCGCTATCCATGAACAAATTAATAAATTAGTTCATGGATTAGTCTCCACCTTTATTGCGCTAGGGTGGGTTTCTGGGTA
GAATAGCGCGCCCTCTGGCT

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEGTKFSQHLLDADKRYLVTAK
LGERTDTSDSDGEVVQTRAIDFTEAQLLTALDFFRGETQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFI
GLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYARMVSLEQLEALVAQAHEQQIDPSVLLDPLL
LPMDTAVADFPEVNVPDAIAPYLMQGQAVRVPVNADLKTDELVRITLGDIRRFVGIGTMNEDGLLAPKRLIVIHDEPAET
D

Sequences:

>Translated_321_residues
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEGTKFSQHLLDADKRYLVTAK
LGERTDTSDSDGEVVQTRAIDFTEAQLLTALDFFRGETQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFI
GLEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYARMVSLEQLEALVAQAHEQQIDPSVLLDPLL
LPMDTAVADFPEVNVPDAIAPYLMQGQAVRVPVNADLKTDELVRITLGDIRRFVGIGTMNEDGLLAPKRLIVIHDEPAET
D
>Mature_320_residues
ARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGEGTKFSQHLLDADKRYLVTAKL
GERTDTSDSDGEVVQTRAIDFTEAQLLTALDFFRGETQQVPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFIG
LEGDELTLDIHCSKGTYIRTIIDDLGEMLGCGAHVIMLRRTQVAQYPYARMVSLEQLEALVAQAHEQQIDPSVLLDPLLL
PMDTAVADFPEVNVPDAIAPYLMQGQAVRVPVNADLKTDELVRITLGDIRRFVGIGTMNEDGLLAPKRLIVIHDEPAETD

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=191, Percent_Identity=37.696335078534, Blast_Score=115, Evalue=4e-26,
Organism=Escherichia coli, GI2367200, Length=313, Percent_Identity=61.9808306709265, Blast_Score=400, Evalue=1e-113,
Organism=Caenorhabditis elegans, GI17553978, Length=276, Percent_Identity=27.536231884058, Blast_Score=81, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6324037, Length=145, Percent_Identity=37.2413793103448, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=276, Percent_Identity=25.7246376811594, Blast_Score=83, Evalue=6e-17,
Organism=Drosophila melanogaster, GI281364189, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281364187, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281364185, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281364183, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI62471759, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17975520, Length=263, Percent_Identity=25.4752851711027, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 35548; Mature: 35417

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHEEECC
GTKFSQHLLDADKRYLVTAKLGERTDTSDSDGEVVQTRAIDFTEAQLLTALDFFRGETQQ
CCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFIGLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAQYPYARMVSLEQLEALVAQAHEQQIDPSVLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LPMDTAVADFPEVNVPDAIAPYLMQGQAVRVPVNADLKTDELVRITLGDIRRFVGIGTMN
CCCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCCC
EDGLLAPKRLIVIHDEPAETD
CCCCCCCCEEEEEECCCCCCC
>Mature Secondary Structure 
ARRPKGRFIDGIVLLDKSTGMSSNFALQRVKRFFNANKAGHTGALDPLATGMLPVCLGE
CCCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHHEEECC
GTKFSQHLLDADKRYLVTAKLGERTDTSDSDGEVVQTRAIDFTEAQLLTALDFFRGETQQ
CCHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHCCCHHH
VPSMYSALKYQGQPLYKYAREGIEVPRESRPITVFELNFIGLEGDELTLDIHCSKGTYIR
HHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEEEEEEEEEEECCCEEEEEEEECCCCCHH
TIIDDLGEMLGCGAHVIMLRRTQVAQYPYARMVSLEQLEALVAQAHEQQIDPSVLLDPLL
HHHHHHHHHHCCCCEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHH
LPMDTAVADFPEVNVPDAIAPYLMQGQAVRVPVNADLKTDELVRITLGDIRRFVGIGTMN
CCCHHHHHCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCCEEEEEHHHHHHHHHCCCCC
EDGLLAPKRLIVIHDEPAETD
CCCCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA