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The map label for this gene is pnp

Identifier: 160876518

GI number: 160876518

Start: 4026186

End: 4028288

Strand: Reverse

Name: pnp

Synonym: Sbal195_3412

Alternate gene names: 160876518

Gene position: 4028288-4026186 (Counterclockwise)

Preceding gene: 160876520

Following gene: 160876517

Centisome position: 75.33

GC content: 45.51

Gene sequence:

>2103_bases
GTGAATCCTATTGTAAAGAGTTTTGAGTATGGTCAACATACAGTCACCCTGGAAACAGGTGTTATAGCGCGTCAAGCAGA
TGCAGCCGTTTTAGCGAGTATGGGTGACACGACTGTGTTGGTCACTGTTGTCGGTAAAAAAGAAGCAGATGCTGGTCGTG
ACTTTTTCCCTCTGACTGTTAACTATCAAGAAAAAACCTACGCAGCAGGTAAAATTCCTGGTGGTTTCTTTAAGCGTGAA
GGCCGTCCTTCAGAAGATGAGACATTAATCGCTCGTCTGATCGACCGTCCAATTCGCCCTCTTTTCCCTAATGGTTTCAA
AAACGAAGTTCAAGTTATCATTACTGTAGTATCTGTCGATCCACAAATCGAGCCAGATATCATCTCTATGATCGGTACTT
CTGCGGCACTGGCTATTTCTGGTATTCCATTCAGCGGTCCTTTAGGTGCTGCACGTGTGGGTTACATTGATGGCGAATAC
GTGTTGAACCCAAGTGTTGCGCAACTTGCAACCAGCCAACTGAACTTAGTGGTTGCTGGTACTGCTGGTGCAGTATTAAT
GGTGGAATCAGAAGCCCAAGCATTGCCAGAAGAAGTGATGTTAGGTTCTGTTGTTTACGGTCACGATCAACAACAAGTGG
TTATCAAAGCGATTGCTGAATTTAAAGCAGAAGCCGGTAAACCAACTTGGGATTGGACTGCACCAACGCAAGATGCCGAT
TTAGTTGCCCAAATCAAAGAGCTTGCTGAAGCTGGTTTAGGCGATGCATATAAAATCCAAGTTAAACAAGATCGTTATGC
TCAAGTTTCTGTTGTTAAAGCAGCAACAAAAGAAGCCTTGTTAGCCAGCAATCCAAGCATTGATTTACGTGAAGTAGACA
ACTTGCTGGGTAGCTTAGAGAAGAAAGTGGTTCGTGGTCGCATCATCCGTGGTGAGCCACGTATCGATGGTCGTGAACCA
GACATGATCCGTGCATTGAGCGTGTTAGCGGGCGTATTGCCACGTACTCACGGTAGTGCATTGTTCACTCGCGGCGAAAC
TCAAGCGTTAGTGACTTGTACTTTAGGTACTGAACGTGATGCACAGAAGATTGACAGCATCATGGGCGAGCGCACTAACC
GTTTCATGCTGCACTATAACTTCCCTCCATACTCTGTTGGTGAAACAGGCATGGTGGGTTCACCTAAGCGCCGCGAAATC
GGTCATGGTAAGTTAGCATGGCGCGGTATCAATGCTGTTATGCCTTCTGCTGCTGAATTCCCATACAGCGTGCGCGTTGT
ATCTGAAATCACTGAATCTAACGGTTCAAGCTCTATGGCGTCAGTATGTGGTACTTCATTAGCTCTGATGGATGCTGGTG
TGCCTATCAAAACTTCTGTAGCGGGTATCGCTATGGGTCTAGTGAAAGAAGGCGATGATTTCGTTGTTCTTTCTGACATC
TTAGGTGATGAAGATCATTTAGGTGATATGGACTTTAAAGTTGCGGGTACGCGTGATGGTATTACTGCACTGCAGATGGA
TATCAAGATCGAAGGTATCACTAAAGAAATCATGGATATCGCACTGCAACAAGCCTATGGCGCACGTGTACATATCCTGA
ACGTGATGGATCAAGCGATTGGTTCACACCGTGATGATATTTCTGACCACGCTCCACGTATCACTGTTATCAAGATCAAC
CCAGAAAAAATTCGTGACGTTATCGGTAAAGGCGGCGCAGTTATCCGTGCTCTTACTGAAGAAACCGGCACTACGATTGA
ACTGGAAGATGACGGTACTGTGAAGATCGCTTCTTCAAACGGCGAAGCAACCAAAGAAGCTATCCGTCGTATCGAAGAAA
TCACTTCAGAAGTTGAAGTGGGTCGTATCTACAACGGTAAAGTTATCCGTATCGTTGATTTCGGTGCGTTCGTGAACATT
CTGCCGGGCAAAGACGGTTTAGTACACATTTCACAAATCAGTGATGAGCGTGTTGCTAACGTGTCTGATCACCTTGAACT
GAACCAAGAAGTTGCTGTTAAAGTAATGGAAGTGGATCGTCAAGGCCGCGTACGTCTGTCAATTAAAGAAGCACAAACTA
AAGAAACTGCCGCTGCTGAATAA

Upstream 100 bases:

>100_bases
AGTTACGTATTTGTCATCATTATCTGCTCTTTATCTATTGATCCGATTTGCAGTATACTTACGCGCGTAATTCAAGGTCA
TTAATTAAGGAATGGGTCAC

Downstream 100 bases:

>100_bases
TGAGTCTTTGACTTATTATTGGCACACACTTTCTAAGTGATTGCATCAAAAGGAGATCAAATGATCTCCTTTTTTGTTTT
TGGCATTAAGAAAGTCAGTA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 700; Mature: 700

Protein sequence:

>700_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEY
VLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQDAD
LVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE

Sequences:

>Translated_700_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEY
VLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQDAD
LVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
>Mature_700_residues
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTVNYQEKTYAAGKIPGGFFKRE
GRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVDPQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEY
VLNPSVAQLATSQLNLVVAGTAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQDAD
LVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLEKKVVRGRIIRGEPRIDGREP
DMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERDAQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREI
GHGKLAWRGINAVMPSAAEFPYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
LGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAIGSHRDDISDHAPRITVIKIN
PEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSNGEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNI
LPGKDGLVHISQISDERVANVSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=716, Percent_Identity=37.7094972067039, Blast_Score=434, Evalue=1e-121,
Organism=Escherichia coli, GI145693187, Length=691, Percent_Identity=73.3719247467438, Blast_Score=1041, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=678, Percent_Identity=32.8908554572271, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI281362905, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651641, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI24651643, Length=711, Percent_Identity=36.8495077355837, Blast_Score=445, Evalue=1e-125,
Organism=Drosophila melanogaster, GI161079377, Length=657, Percent_Identity=36.5296803652968, Blast_Score=405, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_SHEB5 (A3D7K1)

Other databases:

- EMBL:   CP000563
- RefSeq:   YP_001051583.1
- ProteinModelPortal:   A3D7K1
- STRING:   A3D7K1
- GeneID:   4842573
- GenomeReviews:   CP000563_GR
- KEGG:   sbl:Sbal_3234
- NMPDR:   fig|325240.3.peg.3880
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF54814 KH_prok; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 75327; Mature: 75327

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEYVLNPSVAQLATSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHHHCCEEEEEEE
TAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQDAD
CCCEEEEEECHHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLE
HHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAAEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
CEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITVIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
CHHHHHCCHHHEEEEEEECCCCCEEEEEEHHCCCHHCCCC
>Mature Secondary Structure
MNPIVKSFEYGQHTVTLETGVIARQADAAVLASMGDTTVLVTVVGKKEADAGRDFFPLTV
CCCCCCCCCCCCEEEEEECCCEEECCCHHHHHCCCCCEEEEEEECCCCCCCCCCEEEEEE
NYQEKTYAAGKIPGGFFKREGRPSEDETLIARLIDRPIRPLFPNGFKNEVQVIITVVSVD
ECCCCEEECCCCCCCHHCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEEEEECC
PQIEPDIISMIGTSAALAISGIPFSGPLGAARVGYIDGEYVLNPSVAQLATSQLNLVVAG
CCCCHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEECCCEEECCHHHHHHHCCEEEEEEE
TAGAVLMVESEAQALPEEVMLGSVVYGHDQQQVVIKAIAEFKAEAGKPTWDWTAPTQDAD
CCCEEEEEECHHHHCCHHHHHCCEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
LVAQIKELAEAGLGDAYKIQVKQDRYAQVSVVKAATKEALLASNPSIDLREVDNLLGSLE
HHHHHHHHHHCCCCCEEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KKVVRGRIIRGEPRIDGREPDMIRALSVLAGVLPRTHGSALFTRGETQALVTCTLGTERD
HHHHHCEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEEEECCCCCH
AQKIDSIMGERTNRFMLHYNFPPYSVGETGMVGSPKRREIGHGKLAWRGINAVMPSAAEF
HHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEECCHHHCCCCCCC
PYSVRVVSEITESNGSSSMASVCGTSLALMDAGVPIKTSVAGIAMGLVKEGDDFVVLSDI
CEEEHHHHHHHHCCCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEHHH
LGDEDHLGDMDFKVAGTRDGITALQMDIKIEGITKEIMDIALQQAYGARVHILNVMDQAI
CCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHHCCCEEEEHHHHHHHH
GSHRDDISDHAPRITVIKINPEKIRDVIGKGGAVIRALTEETGTTIELEDDGTVKIASSN
CCCCCCHHHCCCEEEEEEECHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCEEEEECCC
GEATKEAIRRIEEITSEVEVGRIYNGKVIRIVDFGAFVNILPGKDGLVHISQISDERVAN
CHHHHHHHHHHHHHHHHHHCCEEECCEEEEEEECCCEEEEECCCCCEEEEEECCCHHHCC
VSDHLELNQEVAVKVMEVDRQGRVRLSIKEAQTKETAAAE
CHHHHHCCHHHEEEEEEECCCCCEEEEEEHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA