Click here to switch to the map view.

The map label for this gene is prfC [H]

Identifier: 160876477

GI number: 160876477

Start: 3980110

End: 3981681

Strand: Reverse

Name: prfC [H]

Synonym: Sbal195_3371

Alternate gene names: 160876477

Gene position: 3981681-3980110 (Counterclockwise)

Preceding gene: 160876478

Following gene: 160876476

Centisome position: 74.46

GC content: 47.2

Gene sequence:

>1572_bases
ATGTCTATAGAAATCAATAAGCGACGCACTTTTGCTATTATATCGCACCCCGACGCGGGTAAAACAACCATTACCGAAAA
AGTGCTGTTATTCGGAAACGCTTTGCAAAAAGCGGGAACGGTTAAAGGCAAAAAATCGGGTCAGCATGCTAAGTCTGACT
GGATGGAAATGGAGAAGGACCGTGGTATTTCTATTACTACGTCTGTGATGCAGTTCCCCTATGGTGGCGCCCTCGTTAAC
CTACTCGACACGCCTGGTCACGAAGACTTCTCTGAAGATACCTATCGTACTCTGACCGCTGTTGACTCCTGTCTGATGGT
CATCGACTCCGCTAAGGGTGTTGAAGAGCGTACCATTAAGTTGATGGAAGTGACACGTCTGCGCGATACGCCGATCGTGA
CTTTTATGAACAAACTCGACCGCGATATCCGTGATCCTATCGATCTGATGGATGAAGTCGAAAGCGTGCTGAATATTGCC
TGCGCACCTATTACTTGGCCTATTGGCAGCGGTAAAGAATTCAAAGGTATTTACCATATACTGCGTGATGAAGTGGTCTT
GTATCAAGGCGGTATGGGACACACTATTCAAGAGCGTCGTGTAATTAAAGGCATTAATAACCCTGATCTTGAGAAAGCGA
TTGGCAGTTACGCTGCTGATTTGCGCGATGAGATGGAATTAGTGCGCGGCGCGTCGCATGAATTTGACCATGCGGCTTTC
TTGAAAGGTGAGCTGACGCCAGTGTTCTTCGGTACCGCGTTAGGTAACTTTGGTGTGGATCATATTCTTGACGGTATCGT
TGAGTGGGCACCTAAGCCATTACCGCGCGAAAGCGATGCACGCGTAGTTATGCCTGAAGAAGAGAAATTCACCGGTTTCG
TGTTCAAGATCCAAGCTAACATGGATCCAAAACACCGTGACCGTGTCGCCTTTATGCGTGTGTGTTCTGGCCGTTACGAG
CAAGGCATGAAAATGCACCATGTGCGTATTGGTAAAGATGTTAACGTTAGCGATGCATTAACCTTTATGGCGGGCGATCG
TGAGCGTGCCGAGGAAGCTTACCCAGGCGATATCATTGGTTTACATAACCATGGCACTATCCGTATTGGCGATACTTTTA
CCCAAGGTGAAAAATTCCGTTTCACTGGCGTGCCTAACTTCGCCCCTGAAATGTTCCGCCGTATTCGTCTGCGCGATCCG
CTTAAGCAAAAGCAGCTCTTAAAAGGCTTAGTACAGTTGTCAGAAGAAGGCGCTGTGCAAGTATTCCGCCCAATCGATAC
TAACGATTTAATTGTTGGCGCCGTGGGTGTGCTGCAGTTTGAAGTGGTTGTTGGTCGTCTTAAGAGCGAATATAACGTTG
AAGCCATTTACGAAGGTATTAGTGTTTCGACCGCTCGCTGGGTTTATTGTAAGGATGAGCGTAAGCTTGAAGAGTTCCGC
CGCAAGTGTAGCCAAAACTTAGCACTCGATGGTGGTGACAACTTAACCTATATTGCGCCAACTATGGTCAACCTGAATCT
TTCGATGGAAAGGTATCCTGATATTGAGTTTGCAAAAACCCGCGAGCATTAA

Upstream 100 bases:

>100_bases
CTATACTCCGCGCCAAGATATCAAATGACAAATCATTATTTTAGTTTTGTGTACAAGCGTGTGTACAAACTGATTGAACA
ACGAGTAAGACTTAATAAAT

Downstream 100 bases:

>100_bases
TTAGGTTGGGTTTAGAATAGTTGTTGAAATGGCGGTGAATTCACCGTCATTTTTGTATCTAATGAAATCTAAAAACGGTT
CTGGATGTGAATGATGGCGA

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MSIEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGALVN
LLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIA
CAPITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHAAF
LKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARVVMPEEEKFTGFVFKIQANMDPKHRDRVAFMRVCSGRYE
QGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDP
LKQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEEFR
RKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Sequences:

>Translated_523_residues
MSIEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGALVN
LLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIA
CAPITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHAAF
LKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARVVMPEEEKFTGFVFKIQANMDPKHRDRVAFMRVCSGRYE
QGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDP
LKQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEEFR
RKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
>Mature_522_residues
SIEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKDRGISITTSVMQFPYGGALVNL
LDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIKLMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIAC
APITWPIGSGKEFKGIYHILRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHAAFL
KGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARVVMPEEEKFTGFVFKIQANMDPKHRDRVAFMRVCSGRYEQ
GMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIGLHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPL
KQKQLLKGLVQLSEEGAVQVFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEEFRR
KCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=491, Percent_Identity=27.4949083503055, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI19923640, Length=509, Percent_Identity=25.9332023575639, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI25306283, Length=393, Percent_Identity=27.4809160305344, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI25306287, Length=481, Percent_Identity=25.3638253638254, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI157426893, Length=172, Percent_Identity=28.4883720930233, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI94966754, Length=139, Percent_Identity=30.9352517985612, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=28.8590604026846, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=77.7351247600768, Blast_Score=865, Evalue=0.0,
Organism=Escherichia coli, GI1789738, Length=499, Percent_Identity=25.250501002004, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1788922, Length=156, Percent_Identity=30.1282051282051, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI48994988, Length=198, Percent_Identity=29.7979797979798, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17533571, Length=484, Percent_Identity=26.8595041322314, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI17556745, Length=183, Percent_Identity=30.6010928961749, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17557151, Length=135, Percent_Identity=31.1111111111111, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=161, Percent_Identity=29.8136645962733, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=479, Percent_Identity=27.1398747390397, Blast_Score=174, Evalue=4e-44,
Organism=Saccharomyces cerevisiae, GI6322359, Length=523, Percent_Identity=24.282982791587, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324707, Length=155, Percent_Identity=30.3225806451613, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320593, Length=155, Percent_Identity=30.3225806451613, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=23.5668789808917, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24582462, Length=474, Percent_Identity=26.7932489451477, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI221458488, Length=502, Percent_Identity=23.5059760956175, Blast_Score=113, Evalue=4e-25,
Organism=Drosophila melanogaster, GI78706572, Length=214, Percent_Identity=28.9719626168224, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24585709, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24585711, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24585713, Length=154, Percent_Identity=31.1688311688312, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58976; Mature: 58845

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKD
CCEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
RGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIK
CCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIACAPITWPIGSGKEFKGIYHI
HHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
LRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHAAF
HHCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
LKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARVVMPEEEKFTGFVFKIQAN
ECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEEEC
MDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIG
CCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHEEEECCHHHHHHCCCCCEEE
LHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEGAVQ
ECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEE
VFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEEFR
EEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHHHHH
RKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SIEINKRRTFAIISHPDAGKTTITEKVLLFGNALQKAGTVKGKKSGQHAKSDWMEMEKD
CEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHC
RGISITTSVMQFPYGGALVNLLDTPGHEDFSEDTYRTLTAVDSCLMVIDSAKGVEERTIK
CCCEEEHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LMEVTRLRDTPIVTFMNKLDRDIRDPIDLMDEVESVLNIACAPITWPIGSGKEFKGIYHI
HHHHHHHCCCHHHHHHHHHCHHHCCHHHHHHHHHHHHHHHCCCEEECCCCCCHHHHHHHH
LRDEVVLYQGGMGHTIQERRVIKGINNPDLEKAIGSYAADLRDEMELVRGASHEFDHAAF
HHCCEEEEECCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
LKGELTPVFFGTALGNFGVDHILDGIVEWAPKPLPRESDARVVMPEEEKFTGFVFKIQAN
ECCCCCCEEEHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCEEEEEEEEC
MDPKHRDRVAFMRVCSGRYEQGMKMHHVRIGKDVNVSDALTFMAGDRERAEEAYPGDIIG
CCCCHHHHHHHHHHHCCHHHCCCEEEEEEECCCCCHHHHHEEEECCHHHHHHCCCCCEEE
LHNHGTIRIGDTFTQGEKFRFTGVPNFAPEMFRRIRLRDPLKQKQLLKGLVQLSEEGAVQ
ECCCCEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCEE
VFRPIDTNDLIVGAVGVLQFEVVVGRLKSEYNVEAIYEGISVSTARWVYCKDERKLEEFR
EEECCCCCCEEEHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECEEEEEEECCHHHHHHHH
RKCSQNLALDGGDNLTYIAPTMVNLNLSMERYPDIEFAKTREH
HHHHCCCEECCCCCEEEEEEEEEEEEECHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA