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The map label for this gene is trmB [H]

Identifier: 160876301

GI number: 160876301

Start: 3766616

End: 3767332

Strand: Reverse

Name: trmB [H]

Synonym: Sbal195_3195

Alternate gene names: 160876301

Gene position: 3767332-3766616 (Counterclockwise)

Preceding gene: 160876304

Following gene: 160876300

Centisome position: 70.45

GC content: 48.12

Gene sequence:

>717_bases
ATGAGCGAAGTCACTACCGCTGAATTTAATGAAGAAGGCAAGTATCTGCGTAAGATCAGAAGCTTTGTCCTAAGAGAAGG
TCGCTTAACTAAAGGCCAAGCCCAAGCTATTGAAAGCCAGTGGCCAACAATGGGGTTAGATTACAGCCCAACGCCATTGG
TTCTGAGCGACGTTTTTGGCCGTGAAGCCGATACTGTGCTGGAAATTGGCTTTGGCATGGGCGCCTCTTTAGTGCAAATG
GCTAAAGACGCACCAGCGCAAAATTTTATTGGTATTGAAGTGCATAAACCGGGTGTCGGTTCTTGCTTAAGCGATGCCGC
CATCGCGGGTGTAACTAATTTACGTGTGTATCATCATGATGCCATGGAAGTATTAGAACATGCGATTGCCGACGGTTCAT
TGGCCCGAGTGCAATTGTTTTTCCCCGATCCTTGGCATAAGAAACGTCATCATAAGCGTCGTATCGTACAAGCTGAATTT
GCTGAACTGATCCGCCGCAAGCTAAAAATTGGCGGCGTGTTCCATATTGCAACCGATTGGGAAGAATACAGCGAGCACAT
GTTGGAAGTGATGCAAGCGGCTCCTGGTTATAAAAACCAGTCGAGTGACGGCACTGTGGTACCACGTCCTGATCATCGCC
CACTGACAAAGTTTGAAGCCCGCGGCCATAGATTGGGTCACGGTGTATGGGATCTGATGTTTGAGCGTATCGCTTAA

Upstream 100 bases:

>100_bases
AATCTTGGTATACTTTCGCCCCATTAGTGCTTGCACTGTATGTTGAACTTTGGATAATGCCCTTCTTTTATGATGGTGCT
GTCCACTAGACGAGGCAAAA

Downstream 100 bases:

>100_bases
TCTAGATATTTCAATTGATTAATTAGTTTTTTAACACAACGGGGAAAGTCACTATGGCAACTAATCGTAGCCGTCGTTTA
CGTAAGAAAATGCGCGTTGA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQM
AKDAPAQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELIRRKLKIGGVFHIATDWEEYSEHMLEVMQAAPGYKNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA

Sequences:

>Translated_238_residues
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQM
AKDAPAQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEF
AELIRRKLKIGGVFHIATDWEEYSEHMLEVMQAAPGYKNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
>Mature_237_residues
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFGREADTVLEIGFGMGASLVQMA
KDAPAQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHDAMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFA
ELIRRKLKIGGVFHIATDWEEYSEHMLEVMQAAPGYKNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=236, Percent_Identity=64.8305084745763, Blast_Score=325, Evalue=2e-90,
Organism=Saccharomyces cerevisiae, GI6320000, Length=148, Percent_Identity=27.7027027027027, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 26755; Mature: 26624

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFG
CCCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVQMAKDAPAQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELIRRKLKIGGVFHIATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCH
EEYSEHMLEVMQAAPGYKNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SEVTTAEFNEEGKYLRKIRSFVLREGRLTKGQAQAIESQWPTMGLDYSPTPLVLSDVFG
CCCCCCHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHC
READTVLEIGFGMGASLVQMAKDAPAQNFIGIEVHKPGVGSCLSDAAIAGVTNLRVYHHD
CCHHHHHHHCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHEEEEHHH
AMEVLEHAIADGSLARVQLFFPDPWHKKRHHKRRIVQAEFAELIRRKLKIGGVFHIATDW
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCH
EEYSEHMLEVMQAAPGYKNQSSDGTVVPRPDHRPLTKFEARGHRLGHGVWDLMFERIA
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA