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The map label for this gene is flgJ [H]

Identifier: 160876198

GI number: 160876198

Start: 3659923

End: 3661065

Strand: Reverse

Name: flgJ [H]

Synonym: Sbal195_3092

Alternate gene names: 160876198

Gene position: 3661065-3659923 (Counterclockwise)

Preceding gene: 160876199

Following gene: 160876197

Centisome position: 68.47

GC content: 48.21

Gene sequence:

>1143_bases
ATGGAAAAGCTTTCAAGTTCATCACATTTTCTTGACCTGGGCGGCTTAGATTCACTCCGCGCCCAGGCTCAGAAGGATGA
GAAGGGCGCCTTGAAAAAGGTCGCCCAACAATTTGAAGGTATTTTCGTGCAAATGCTGATGAAAAGTATGCGCGATGCCA
ATGCGGTTTTTCAGTCTGATAGTCCTCTCAATAGCCAATACACTAAGTTTTACGAGCAGATGCGCGATCAGCAGTTATCG
GTCGATTTATCTGACAAAGGGGTATTGGGCCTTGCCGATATGATGGTGCAGCAACTTTCCCCTGAAACCAGTCATTTCAC
GCCAGCATCGGTATTACGCGACGATGGCGGTATGAAAATGCTCCATGATGCTAAAGCGCTTAACGTGCCAATGCAGAGTG
CAGTCGCGCCAGCGGCTGAATCCACAGTTGTTAATCATAGCGTACCCGCTGCCATTGCTCGACCAGCATTTGAGCCCGAT
AGAGCCATGGATGCAAACTCATCAATGGACATTGATACGCCTAATCGTTTACTGGCAATTGATACACCTAAGCCAAGCTG
GAGCGAACAGTCACTTTCTGTCGTCGACCCTGTGATTAGCGGCCAAGTTTTGCCGACGGCCGCCTTTAGGGAAACCCAGA
AGATAGTCCGTTTCGGTAGTCGAGAAGAGTTTTTGGCGACCTTATATCCACACGCTGAAAAAGCGGCAAAAGCATTAGGC
ACTCAACCAGAAGTATTGTTGGCGCAATCGGCTCTTGAAACCGGCTGGGGACAAAAAATTGTTCGCGGCAATAACGGCGC
GCCAAGTCATAACCTATTCAATATTAAAGCTGATCGCCGTTGGCAGGGCGATAAGGCGAACGTCAGCACCTTAGAATTCG
AACACGGTGTGGCAGTGCAGCAAAAAGCCGACTTCCGTGTTTACAGTGACTTTGAGCATAGCTTCAACGATTTTGTGTCC
TTCATTGCCGAAGGCGATCGTTATCAAGATGCGAAGAAAGTGGCGGCTTCGCCAACTCAGTTTATTCGAGCTTTGCAGGA
TGCGGGTTATGCCACAGATCCTAGATATGCTGAAAAAGTCATTAAAGTGATGCAGTCCATCAGTGAAGAGCTGAAATCAA
TTCTGCCAGGAGAAGACAAATGA

Upstream 100 bases:

>100_bases
GTGCCGCCCCCTCAGATGTGTTAGCAATACTTGAAGCGTTAAAAGTGGCAGGAGCTTTGCATGGTGAACTTATCATCATC
TAATTTCTGTCGGGAAAATC

Downstream 100 bases:

>100_bases
TGAACCTTACAACCCTGTTTGTTCATAGGAGTGTCAACTAATGGCCGTTGATCTGCTGAATATTGCGCGAACTGGGGTGC
TTGCCTCCCAGTCGCAACTG

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGIFVQMLMKSMRDANAVFQSDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPETSHFTPASVLRDDGGMKMLHDAKALNVPMQSAVAPAAESTVVNHSVPAAIARPAFEPD
RAMDANSSMDIDTPNRLLAIDTPKPSWSEQSLSVVDPVISGQVLPTAAFRETQKIVRFGSREEFLATLYPHAEKAAKALG
TQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEHGVAVQQKADFRVYSDFEHSFNDFVS
FIAEGDRYQDAKKVAASPTQFIRALQDAGYATDPRYAEKVIKVMQSISEELKSILPGEDK

Sequences:

>Translated_380_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGIFVQMLMKSMRDANAVFQSDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPETSHFTPASVLRDDGGMKMLHDAKALNVPMQSAVAPAAESTVVNHSVPAAIARPAFEPD
RAMDANSSMDIDTPNRLLAIDTPKPSWSEQSLSVVDPVISGQVLPTAAFRETQKIVRFGSREEFLATLYPHAEKAAKALG
TQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEHGVAVQQKADFRVYSDFEHSFNDFVS
FIAEGDRYQDAKKVAASPTQFIRALQDAGYATDPRYAEKVIKVMQSISEELKSILPGEDK
>Mature_380_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGIFVQMLMKSMRDANAVFQSDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPETSHFTPASVLRDDGGMKMLHDAKALNVPMQSAVAPAAESTVVNHSVPAAIARPAFEPD
RAMDANSSMDIDTPNRLLAIDTPKPSWSEQSLSVVDPVISGQVLPTAAFRETQKIVRFGSREEFLATLYPHAEKAAKALG
TQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEHGVAVQQKADFRVYSDFEHSFNDFVS
FIAEGDRYQDAKKVAASPTQFIRALQDAGYATDPRYAEKVIKVMQSISEELKSILPGEDK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=156, Percent_Identity=44.8717948717949, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 41828; Mature: 41828

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGIFVQMLMKSMRDANAVFQSD
CCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPETSHFTPASVLRDDGGMKM
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHH
LHDAKALNVPMQSAVAPAAESTVVNHSVPAAIARPAFEPDRAMDANSSMDIDTPNRLLAI
HHHHHHHCCCHHHHHCCHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
DTPKPSWSEQSLSVVDPVISGQVLPTAAFRETQKIVRFGSREEFLATLYPHAEKAAKALG
ECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHC
TQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEHGVAVQ
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEC
QKADFRVYSDFEHSFNDFVSFIAEGDRYQDAKKVAASPTQFIRALQDAGYATDPRYAEKV
CCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
IKVMQSISEELKSILPGEDK
HHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGIFVQMLMKSMRDANAVFQSD
CCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPETSHFTPASVLRDDGGMKM
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCHH
LHDAKALNVPMQSAVAPAAESTVVNHSVPAAIARPAFEPDRAMDANSSMDIDTPNRLLAI
HHHHHHHCCCHHHHHCCHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEE
DTPKPSWSEQSLSVVDPVISGQVLPTAAFRETQKIVRFGSREEFLATLYPHAEKAAKALG
ECCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHC
TQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEHGVAVQ
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEC
QKADFRVYSDFEHSFNDFVSFIAEGDRYQDAKKVAASPTQFIRALQDAGYATDPRYAEKV
CCCCCEEHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHH
IKVMQSISEELKSILPGEDK
HHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]