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The map label for this gene is emrD [H]

Identifier: 160875496

GI number: 160875496

Start: 2814191

End: 2815423

Strand: Direct

Name: emrD [H]

Synonym: Sbal195_2384

Alternate gene names: 160875496

Gene position: 2814191-2815423 (Clockwise)

Preceding gene: 160875495

Following gene: 160875499

Centisome position: 52.63

GC content: 48.99

Gene sequence:

>1233_bases
ATGTCAGGCACGCCACACTCTCCTATTCATCACGATGTCGCTCCAGCGGCGAGTCAGTCTAGTTTACTGTTAATGATTGT
CTTTATGGTCGCCTGTGGCCAAATGGCGCAAACGATTTTTGTGCCTGCCCTTCCGCAAATTGCACTCGGGCTCCATGTGG
ACGCCAGTCAGCTACAAGCAGTGATGGCCTGTTACTTACTCTCCTATGGGATGTTTCAATTTGTGTATGGCCCAGTGTCA
GATCGCATTGGCCGCAAAATGCCACTCGTCGTGGGTATCGCTATTTTCATCATAGGAGCAGTGTTTGCGGCTCAGGCCAC
AAGCTTTAACCAACTGATTATGGCGAGCCTTATCCAAGGATTAGGCACAGCCAGCGCGGGCGCTTTATGCCGGAGCATTC
CCCGCGATCATTACTTTGGCGACCGACTGGTTAAATTTAATAGTTATGTTTCTATGGCCGTAGTGTTTTTACCTTTACTG
GCACCATTTATCGGTAGCGCCAGTGCGGCCTACTTCGGCTGGCAAGCCGTGTACTGGGTGTTAGCAGGATTCGGTGCTGT
GGTCTGGCTGTTATTGATATTCGCCTTTAAAGAGTCATTACCTAAGGCGCAGCGGGAACATCAAGCCGTTATCGCATCCT
ATCGTCATGTCCTGCGCCATAGAGCATTTCGAGCTTACTTGTTTTCATTGATCGCAACCTTTGCGGGTATTGCGGCGTTT
GAAGCCATTGCTGGCGTACTCTATGGCCAATATCTTGGACTGACACCCTTCATGGTCAGTTGTTATTTTGTGGCGCCGAT
TCCAGGCTATTTATTGGGCGCGCTGATTGCAGGTCGACAAAACAAGCCGCAAAGAGTTATCTATCAAGGTGTTGCACTGC
TCGGCGCTGGTGCCTTATTTATGTTGATCCCGGGGCTTTTTAATTTAGTCGTCAGTTGGAGTTTACTGGTTGGATCGATT
TTATTCTTCAGTGGCGCCGGTATGCTGTTTCCTACCCTGACCTCGGCGGCTTTAGAGCCATTTCCACGTCAAGCAGGAAT
TGCGGGGGCATTACTGGGAGGATTACAAAATTTTGGTGCCGGACTTGCCGCATTGACTATGTCTTTAGTGCCTATGGGCG
GCCAGTTGAGCATAGGTTTATTAAGCGCTGCCATGGTGGTGTTAGTGATTTTGTCTTTGTATTACGCCAGAGATCAAAAT
GACACTCACCACAATTTGATGCCACAGATTTAA

Upstream 100 bases:

>100_bases
AAAAATTATTTCATCAAATCAATATGATTGACACTTGAAGTTACGCTTTTAGGGAGTAAGATGCGCGCAAATACCTTAAG
TGACCTTTTTCGGAAATATC

Downstream 100 bases:

>100_bases
TGTCCTAAATTTAAGATCCTAGATTTAAAACCATAGATTTAATGCCATAGATTTAATGCCGAAAAATTGAAGCGATAACG
TTCTTAGCTTAAGCAAAATC

Product: multidrug resistance protein D

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 410; Mature: 409

Protein sequence:

>410_residues
MSGTPHSPIHHDVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGMFQFVYGPVS
DRIGRKMPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLL
APFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHRAFRAYLFSLIATFAGIAAF
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVSWSLLVGSI
LFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAAMVVLVILSLYYARDQN
DTHHNLMPQI

Sequences:

>Translated_410_residues
MSGTPHSPIHHDVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGMFQFVYGPVS
DRIGRKMPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLL
APFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHRAFRAYLFSLIATFAGIAAF
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVSWSLLVGSI
LFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAAMVVLVILSLYYARDQN
DTHHNLMPQI
>Mature_409_residues
SGTPHSPIHHDVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQAVMACYLLSYGMFQFVYGPVSD
RIGRKMPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQGLGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLLA
PFIGSASAAYFGWQAVYWVLAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHRAFRAYLFSLIATFAGIAAFE
AIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALFMLIPGLFNLVVSWSLLVGSIL
FFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGAGLAALTMSLVPMGGQLSIGLLSAAMVVLVILSLYYARDQND
THHNLMPQI

Specific function: Multidrug resistance pump that participates in a low energy shock adaptative response [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily [H]

Homologues:

Organism=Escherichia coli, GI87082312, Length=345, Percent_Identity=43.4782608695652, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788509, Length=329, Percent_Identity=27.0516717325228, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1790146, Length=386, Percent_Identity=28.2383419689119, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994889, Length=389, Percent_Identity=25.1928020565553, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790794, Length=299, Percent_Identity=23.0769230769231, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 43775; Mature: 43643

Theoretical pI: Translated: 9.50; Mature: 9.50

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SGTPHSPIHHDVAPAASQSSLLLMIVFMVACGQMAQTIFVPALPQIALGLHVDASQLQA
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHH
VMACYLLSYGMFQFVYGPVSDRIGRKMPLVVGIAIFIIGAVFAAQATSFNQLIMASLIQG
HHHHHHHHHHHHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGTASAGALCRSIPRDHYFGDRLVKFNSYVSMAVVFLPLLAPFIGSASAAYFGWQAVYWV
CCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LAGFGAVVWLLLIFAFKESLPKAQREHQAVIASYRHVLRHRAFRAYLFSLIATFAGIAAF
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAIAGVLYGQYLGLTPFMVSCYFVAPIPGYLLGALIAGRQNKPQRVIYQGVALLGAGALF
HHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
MLIPGLFNLVVSWSLLVGSILFFSGAGMLFPTLTSAALEPFPRQAGIAGALLGGLQNFGA
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHCCHHHHHHHHHHHHHH
GLAALTMSLVPMGGQLSIGLLSAAMVVLVILSLYYARDQNDTHHNLMPQI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8240355; 7686882; 9278503 [H]