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The map label for this gene is pleD [H]

Identifier: 160875470

GI number: 160875470

Start: 2779120

End: 2780760

Strand: Direct

Name: pleD [H]

Synonym: Sbal195_2358

Alternate gene names: 160875470

Gene position: 2779120-2780760 (Clockwise)

Preceding gene: 160875469

Following gene: 160875481

Centisome position: 51.97

GC content: 46.62

Gene sequence:

>1641_bases
ATGAGTTTTGAAGATTCGTTAGAGCAACTTAAACGACAGTTTTTACATGCACTGCCGGATAAAAAGAAACAAATCATTAC
CTTGTGGATTTCATTGCGTAAGAATTGGAATGCACACATGCTGTCTGCTGTGTATCGCGAGGTGCATAACTTAAAAGGGT
CGAGCGAGACCTTTGGATTGTCGGAGACCAAACACATAGTCGATAAACTTGAGCTGCAGCTTAAAGGGTTGCTCAATGCG
CCAGCTCCCGAACTTCCTACTATCAAAGGCTTAGATACCCTCTTCCACCAGTTGTTGCAAAACAATATGCGCGGTCAACC
GTCGCCGCCTGTGGTAGAGGCGCAAATGCAGCAAAGTCCCTATAAACCGGCTAAGGCGCGGCATGATCACCATATTGCCA
TCATAGAAGACGATGGTAATGTCGGCGCAATGATCACAAAGCAACTGCGAGAGTTCGGTTTTAGTGTGCAGCATTTTTTG
AATTTCACGAGCTTTCTGGTGGTGCAAAATGAAACGCCCTTCGATCTTATTCTATTGGATTTAATTCTGCCCGATTGGAC
TGAAGAGGCCTTGTTCGAAGCCGCCACTGAGTTTGAGAAGAACAATACCCGAGTGTTTGTTTTATCCTCGCGGGGTGATT
TTGACATGCGGTTGCTGGCCATTCGTGCCAACGTTAGCGAATATTTTGTCAAACCTGCGGAAACCACTTTATTGGTGCGC
AAAATCCATCAATCTCTCAAGATGTCAGAGAAGCAGCCCTTGAAGGTGTTACTGGTGGATGATCAACAGTCTATGGTGGA
TTATTTTTCAAGCCTGTTACGCAGTCACGGTTTGATGGTGAAAGGGTTATCGAGCGCCGAGCAAGTTTTGCCTGCCCTTG
AACAGTTTGAGCCTGATCTGTTCATCTTCGATTTATACATGCCTGAGGTGAACGGGCTTGAGCTGGCAAAGATGATACGC
CAACTGGATAAATACACCTCTAGCCCAATTCTCGTGTTGAGCTCTGACGATACCATGCAGAATAAAGTCAGCATCATACA
AGCGGGATCAGATGACTTAATTTCAAAGCAAACTGCGCCGAGCTTATTTGTCGCGCAAGTGATATCCCGCGCCCAACGTG
GCCATGATATTCGCAGTTCGGCAAGCCGCGACAGCTTAACGGGATTGTTAAACCATACCCAGATTCTGGTTGCCGCGAGG
CGCTGCTATAACGTGGCGAGGCGAATTAACTCTCAGGTTTGTATCGCCATGTTCGATTTGGACCACTTCAAAAAAGTGAA
CGACACCTATGGCCATGCCGGTGGTGACAAGGTGTTGCTGGCCTTTGCCCATCTATTACAACAATCCTTACGGCAAACTG
ACTTTACCGGTCGCTATGGCGGCGAGGAATTTATGTTAGTGCTGCCAGATATGCAAAAACCATTGGCAATAGCTAAGTTA
AATGCGATCCGCGAGAGCTTTAGCCATATTGTCTTTGTCGAAGATGGCGTCGAGTTTAAGGTGACGCTCAGCGGCGGTTT
AGTGTTCTCGGACGAATGTGAGGAGTTTCAAGATTGTCTATTACTGGCAGATAAAAACCTTTACGAGGCGAAACGTTTAG
GCCGAAATCAATTAGTGGTTTCACCAAGTGAGCAGGAATGA

Upstream 100 bases:

>100_bases
TGAAAACAGTGTCAATTTCAGTATAAACGGGTTTTATCGTCCCGAGGTAGCGACCACACGGAGAAACCTAGGGCTCAATC
ATTTAGGATATCTATTGCCT

Downstream 100 bases:

>100_bases
ACGCCATTCACTCATCCGGTGCATGAGTCTGGCCGTAATGTCATTCGTTTGGCTCACCGCCATATTGATGATTACTTTTT
AAGCACGTTAAAAGCAGCAA

Product: response regulator receiver modulated diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSFEDSLEQLKRQFLHALPDKKKQIITLWISLRKNWNAHMLSAVYREVHNLKGSSETFGLSETKHIVDKLELQLKGLLNA
PAPELPTIKGLDTLFHQLLQNNMRGQPSPPVVEAQMQQSPYKPAKARHDHHIAIIEDDGNVGAMITKQLREFGFSVQHFL
NFTSFLVVQNETPFDLILLDLILPDWTEEALFEAATEFEKNNTRVFVLSSRGDFDMRLLAIRANVSEYFVKPAETTLLVR
KIHQSLKMSEKQPLKVLLVDDQQSMVDYFSSLLRSHGLMVKGLSSAEQVLPALEQFEPDLFIFDLYMPEVNGLELAKMIR
QLDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVAQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAAR
RCYNVARRINSQVCIAMFDLDHFKKVNDTYGHAGGDKVLLAFAHLLQQSLRQTDFTGRYGGEEFMLVLPDMQKPLAIAKL
NAIRESFSHIVFVEDGVEFKVTLSGGLVFSDECEEFQDCLLLADKNLYEAKRLGRNQLVVSPSEQE

Sequences:

>Translated_546_residues
MSFEDSLEQLKRQFLHALPDKKKQIITLWISLRKNWNAHMLSAVYREVHNLKGSSETFGLSETKHIVDKLELQLKGLLNA
PAPELPTIKGLDTLFHQLLQNNMRGQPSPPVVEAQMQQSPYKPAKARHDHHIAIIEDDGNVGAMITKQLREFGFSVQHFL
NFTSFLVVQNETPFDLILLDLILPDWTEEALFEAATEFEKNNTRVFVLSSRGDFDMRLLAIRANVSEYFVKPAETTLLVR
KIHQSLKMSEKQPLKVLLVDDQQSMVDYFSSLLRSHGLMVKGLSSAEQVLPALEQFEPDLFIFDLYMPEVNGLELAKMIR
QLDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVAQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAAR
RCYNVARRINSQVCIAMFDLDHFKKVNDTYGHAGGDKVLLAFAHLLQQSLRQTDFTGRYGGEEFMLVLPDMQKPLAIAKL
NAIRESFSHIVFVEDGVEFKVTLSGGLVFSDECEEFQDCLLLADKNLYEAKRLGRNQLVVSPSEQE
>Mature_545_residues
SFEDSLEQLKRQFLHALPDKKKQIITLWISLRKNWNAHMLSAVYREVHNLKGSSETFGLSETKHIVDKLELQLKGLLNAP
APELPTIKGLDTLFHQLLQNNMRGQPSPPVVEAQMQQSPYKPAKARHDHHIAIIEDDGNVGAMITKQLREFGFSVQHFLN
FTSFLVVQNETPFDLILLDLILPDWTEEALFEAATEFEKNNTRVFVLSSRGDFDMRLLAIRANVSEYFVKPAETTLLVRK
IHQSLKMSEKQPLKVLLVDDQQSMVDYFSSLLRSHGLMVKGLSSAEQVLPALEQFEPDLFIFDLYMPEVNGLELAKMIRQ
LDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAPSLFVAQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARR
CYNVARRINSQVCIAMFDLDHFKKVNDTYGHAGGDKVLLAFAHLLQQSLRQTDFTGRYGGEEFMLVLPDMQKPLAIAKLN
AIRESFSHIVFVEDGVEFKVTLSGGLVFSDECEEFQDCLLLADKNLYEAKRLGRNQLVVSPSEQE

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=194, Percent_Identity=34.5360824742268, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1786584, Length=245, Percent_Identity=26.1224489795918, Blast_Score=95, Evalue=1e-20,
Organism=Escherichia coli, GI87082007, Length=164, Percent_Identity=34.1463414634146, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI145693134, Length=162, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI87081881, Length=165, Percent_Identity=32.1212121212121, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1788381, Length=187, Percent_Identity=29.9465240641711, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788956, Length=165, Percent_Identity=32.7272727272727, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1787802, Length=128, Percent_Identity=30.46875, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI87081977, Length=84, Percent_Identity=39.2857142857143, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788085, Length=163, Percent_Identity=27.6073619631902, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI87081974, Length=151, Percent_Identity=29.8013245033113, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787816, Length=126, Percent_Identity=31.7460317460317, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1787056, Length=127, Percent_Identity=31.496062992126, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787541, Length=207, Percent_Identity=27.536231884058, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 61774; Mature: 61643

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: PS50894 HPT ; PS50110 RESPONSE_REGULATORY ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFEDSLEQLKRQFLHALPDKKKQIITLWISLRKNWNAHMLSAVYREVHNLKGSSETFGL
CCHHHHHHHHHHHHHHHCCCCCHHEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
SETKHIVDKLELQLKGLLNAPAPELPTIKGLDTLFHQLLQNNMRGQPSPPVVEAQMQQSP
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCC
YKPAKARHDHHIAIIEDDGNVGAMITKQLREFGFSVQHFLNFTSFLVVQNETPFDLILLD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCHHEEEEEECCCCHHHHHHH
LILPDWTEEALFEAATEFEKNNTRVFVLSSRGDFDMRLLAIRANVSEYFVKPAETTLLVR
HHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCHHHHHCCCCHHHHHHH
KIHQSLKMSEKQPLKVLLVDDQQSMVDYFSSLLRSHGLMVKGLSSAEQVLPALEQFEPDL
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCE
FIFDLYMPEVNGLELAKMIRQLDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP
EEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCEEEEECCCCHHHHHHCCC
SLFVAQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARRCYNVARRINSQVCIAMFDL
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECH
DHFKKVNDTYGHAGGDKVLLAFAHLLQQSLRQTDFTGRYGGEEFMLVLPDMQKPLAIAKL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHH
NAIRESFSHIVFVEDGVEFKVTLSGGLVFSDECEEFQDCLLLADKNLYEAKRLGRNQLVV
HHHHHHHCEEEEEECCCEEEEEECCCEEECHHHHHHHHHHHHHCCCHHHHHHCCCCCEEE
SPSEQE
CCCCCC
>Mature Secondary Structure 
SFEDSLEQLKRQFLHALPDKKKQIITLWISLRKNWNAHMLSAVYREVHNLKGSSETFGL
CHHHHHHHHHHHHHHHCCCCCHHEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCCCCCCC
SETKHIVDKLELQLKGLLNAPAPELPTIKGLDTLFHQLLQNNMRGQPSPPVVEAQMQQSP
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCC
YKPAKARHDHHIAIIEDDGNVGAMITKQLREFGFSVQHFLNFTSFLVVQNETPFDLILLD
CCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHCCHHHHHHCHHEEEEEECCCCHHHHHHH
LILPDWTEEALFEAATEFEKNNTRVFVLSSRGDFDMRLLAIRANVSEYFVKPAETTLLVR
HHCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCEEEEEEECCHHHHHCCCCHHHHHHH
KIHQSLKMSEKQPLKVLLVDDQQSMVDYFSSLLRSHGLMVKGLSSAEQVLPALEQFEPDL
HHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEECCCHHHHHHHHHHHCCCCE
FIFDLYMPEVNGLELAKMIRQLDKYTSSPILVLSSDDTMQNKVSIIQAGSDDLISKQTAP
EEEEEECCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCHHHCEEEEECCCCHHHHHHCCC
SLFVAQVISRAQRGHDIRSSASRDSLTGLLNHTQILVAARRCYNVARRINSQVCIAMFDL
HHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECH
DHFKKVNDTYGHAGGDKVLLAFAHLLQQSLRQTDFTGRYGGEEFMLVLPDMQKPLAIAKL
HHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCHHHHHH
NAIRESFSHIVFVEDGVEFKVTLSGGLVFSDECEEFQDCLLLADKNLYEAKRLGRNQLVV
HHHHHHHCEEEEEECCCEEEEEECCCEEECHHHHHHHHHHHHHCCCHHHHHHCCCCCEEE
SPSEQE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA