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The map label for this gene is pdhC [H]

Identifier: 160875314

GI number: 160875314

Start: 2613953

End: 2615578

Strand: Direct

Name: pdhC [H]

Synonym: Sbal195_2201

Alternate gene names: 160875314

Gene position: 2613953-2615578 (Clockwise)

Preceding gene: 160875313

Following gene: 160875315

Centisome position: 48.88

GC content: 49.75

Gene sequence:

>1626_bases
ATGATTAAAGATTTTATTTTGCCGGATATTGGCGAAGGTGTAGTGGAATGTGAATTGGTTGAATGGTTGGTCAAAGAAGG
CGACACAGTCGTTGAAGATCAGCCGATTGCCGATGTCATGACAGACAAAGCCCTAGTGCAAATCCCTGCGCCTTTTGCGG
GCGTGGTCACTAAGCTGTATTACGCTAAGGGCGATATTGCGAAAGTGCATGCTCCGCTCTATGCCGTACAAATTGAAGGT
GCCGTTGAAATCGCAGGTGAAGAATCTGTTGCGGCTGAACCTGCAGCGACGACTGCAAAAGTGACTGAGCCTGTGGTGGC
AACAACTGCCAATACGTCATCCAGTTCATCCGTCAGTATTGAAGAATTCTTACTACCGGATATCGGCGAAGGCATTGTTG
AATGTGAACTCGTTGAATGGCTAGTGAGCGAAGGTGATTGGGTTGAGGAAGATCAACCGATTGCCGACGTAATGACAGAT
AAAGCGCTGGTGCAAATTCCTGCCATTAAAGCGGGTAAAATTGCCAAACTGCATTATCGCAAAGGTCAGCTAGCTAAAGT
ACATGCGCCTTTATTTGCCATTGAAGTTAAGCATATGGCTTCTGCACCTGCGGCGACAACAAACACTGATACTGTGGCCA
ATGCGGCACCAACAGCGCAGATTGTGAGTGCAGAACTTGCTCGCCAAGGTAAAGCCTTAGCCAGCCCTGCGGTTCGCCGG
ATGGCGCGGTCGTTAGATATCGATTTGAGCCAAGTACCTGGCACAGGAAAGCACGGCCGTGTGTATAAGGAAGACATCAC
TCGCTTCCAACAAGGCGCGAGCAATGTTTCTACTCCAAGTACGACTCAGGTAAAAGAGGCGCCAGCTCAAGCGACTCAAG
CATCTCAAACCCAAGTCCCAACATCGACAGTGACTCAACGCGCTGACACTGTTGAGCCTATTCGCGGCGTAAAAGCTGTG
ATGGCACGCATGATGGTTGAGTCGGTGTCGAGCATTCCGCACTTTACCTATTGCGAAGAGTTTGATTTAACAGACTTAGT
TGCACTGCGTGAAAGCATGAAAGTGAAGTATTCATCCGATGTAGTGAAGCTGACTATGATGCCTTTCTTCATGAAGTCGA
TGTCGCTCGCGTTAAGTCAGTTCCCTGTGATGAATAGCCAAGTGAATGCTGATTGCACTGAGCTGACTTACAAGGCGCGC
CACAATATCGGCATGGCGGTGGATTCTAAAGTCGGTCTCTTGGTGCCAAACATCAAAGATGTGCAGGACAAGAGCATTTT
AGAAGTGGCGGCAGAAATCACCCGCCTGACCCAAGCAGCCCGCAGTGGCCGCGTGGCGCCAGCGGATCTGAAAGACGGCA
CGATTTCCATCTCTAACATTGGCGCTTTAGGTGGCACAGTGGCGACGCCTATTATCAATAAACCTGAAGTGGCGATTGTG
GCCTTAGGTAAGTTGCAAACCTTGCCGCGTTTTAACGCGAAAGGGGAAGTCGAAGCCCGCCAAATCATGCAAGTAAGCTG
GTCTGGCGACCACAGAGTGATCGATGGCGGTACCATTGCTCGCTTCTGTAATCTGTGGAAACAATATCTGGAGCAGCCGC
AAGAGATGTTATTGGCCATGCGCTAA

Upstream 100 bases:

>100_bases
AGATACCCCGTACCCACTCATTCATGAAAAAGAATACATGCCTGATGCGTTCAAAACGTTTGAAGCCATCAAAGCATCAG
TGACCTTCTAGGAGTCCGGT

Downstream 100 bases:

>100_bases
GCCGCTGGCACGCAGATTTGACAGTAAAAGGACGCTTTAGAGCGTCCTTTTTTATTTGCTTTGCACAAATGGGTTAATCT
TTTGATCCCGATTACGTATA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2; S complex, 48 kDa subunit [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVEIAGEESVAAEPAATTAKVTEPVVATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVKHMASAPAATTNTDTVANAAPTAQIVSAELARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSTPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDVVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNIGMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR

Sequences:

>Translated_541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVEIAGEESVAAEPAATTAKVTEPVVATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVKHMASAPAATTNTDTVANAAPTAQIVSAELARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSTPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDVVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNIGMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR
>Mature_541_residues
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLYYAKGDIAKVHAPLYAVQIEG
AVEIAGEESVAAEPAATTAKVTEPVVATTANTSSSSSVSIEEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTD
KALVQIPAIKAGKIAKLHYRKGQLAKVHAPLFAIEVKHMASAPAATTNTDTVANAAPTAQIVSAELARQGKALASPAVRR
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSTPSTTQVKEAPAQATQASQTQVPTSTVTQRADTVEPIRGVKAV
MARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSDVVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKAR
HNIGMAVDSKVGLLVPNIKDVQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAMR

Specific function: The B.subtilis PDH complex possesses also branched-chain 2-oxoacid dehydrogenase (BCDH) activity [H]

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=423, Percent_Identity=44.2080378250591, Blast_Score=354, Evalue=1e-97,
Organism=Homo sapiens, GI31711992, Length=577, Percent_Identity=24.7833622183709, Blast_Score=140, Evalue=3e-33,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=27.9017857142857, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI203098753, Length=447, Percent_Identity=26.8456375838926, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI19923748, Length=235, Percent_Identity=30.2127659574468, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI260898739, Length=138, Percent_Identity=36.231884057971, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786305, Length=554, Percent_Identity=30.14440433213, Blast_Score=212, Evalue=6e-56,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=26.2295081967213, Blast_Score=156, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17537937, Length=437, Percent_Identity=38.6727688787185, Blast_Score=312, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI25146366, Length=489, Percent_Identity=28.0163599182004, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17560088, Length=440, Percent_Identity=27.7272727272727, Blast_Score=131, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI17538894, Length=327, Percent_Identity=24.1590214067278, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6320352, Length=465, Percent_Identity=24.7311827956989, Blast_Score=144, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6324258, Length=446, Percent_Identity=26.6816143497758, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859875, Length=429, Percent_Identity=40.7925407925408, Blast_Score=318, Evalue=6e-87,
Organism=Drosophila melanogaster, GI20129315, Length=313, Percent_Identity=26.1980830670927, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24582497, Length=439, Percent_Identity=24.373576309795, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24645909, Length=198, Percent_Identity=30.8080808080808, Blast_Score=98, Evalue=1e-20,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 58251; Mature: 58251

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCHHHHHHHHH
YAKGDIAKVHAPLYAVQIEGAVEIAGEESVAAEPAATTAKVTEPVVATTANTSSSSSVSI
HCCCCCEEEECCEEEEEECCEEEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCH
EEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTDKALVQIPAIKAGKIAKLHYR
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEC
KGQLAKVHAPLFAIEVKHMASAPAATTNTDTVANAAPTAQIVSAELARQGKALASPAVRR
CCCCEEEECCEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCHHHHH
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSTPSTTQVKEAPAQATQASQTQVP
HHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCHHCCCC
TSTVTQRADTVEPIRGVKAVMARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEECCC
VVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKARHNIGMAVDSKVGLLVPNIKD
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCCCCC
VQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEE
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAM
EECHHHCCCCCCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCHHHHHHHC
R
C
>Mature Secondary Structure
MIKDFILPDIGEGVVECELVEWLVKEGDTVVEDQPIADVMTDKALVQIPAPFAGVVTKLY
CCCCCCCCCCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHCCCCEEEECCCHHHHHHHHH
YAKGDIAKVHAPLYAVQIEGAVEIAGEESVAAEPAATTAKVTEPVVATTANTSSSSSVSI
HCCCCCEEEECCEEEEEECCEEEECCCCCCCCCCCCCHHHHCCCEEEEECCCCCCCCCCH
EEFLLPDIGEGIVECELVEWLVSEGDWVEEDQPIADVMTDKALVQIPAIKAGKIAKLHYR
HHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCEEEEEEC
KGQLAKVHAPLFAIEVKHMASAPAATTNTDTVANAAPTAQIVSAELARQGKALASPAVRR
CCCCEEEECCEEEEEHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCHHHHH
MARSLDIDLSQVPGTGKHGRVYKEDITRFQQGASNVSTPSTTQVKEAPAQATQASQTQVP
HHHHCCCCHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHCCHHHHHCHHCCCC
TSTVTQRADTVEPIRGVKAVMARMMVESVSSIPHFTYCEEFDLTDLVALRESMKVKYSSD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCEEECCC
VVKLTMMPFFMKSMSLALSQFPVMNSQVNADCTELTYKARHNIGMAVDSKVGLLVPNIKD
EEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCEEECCCCCEECCCCCC
VQDKSILEVAAEITRLTQAARSGRVAPADLKDGTISISNIGALGGTVATPIINKPEVAIV
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCCCEEEE
ALGKLQTLPRFNAKGEVEARQIMQVSWSGDHRVIDGGTIARFCNLWKQYLEQPQEMLLAM
EECHHHCCCCCCCCCCCCHHHHEEEEECCCEEEECCHHHHHHHHHHHHHHHCHHHHHHHC
R
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1697575; 8969500; 9384377 [H]