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The map label for this gene is ybfF [H]

Identifier: 160875307

GI number: 160875307

Start: 2606300

End: 2607076

Strand: Reverse

Name: ybfF [H]

Synonym: Sbal195_2194

Alternate gene names: 160875307

Gene position: 2607076-2606300 (Counterclockwise)

Preceding gene: 160875310

Following gene: 160875306

Centisome position: 48.76

GC content: 48.26

Gene sequence:

>777_bases
ATGAACTTTGTTTCTACAGGCCAAGGACCCGCAGTACTCTTGATCCACGGTTTGTTCGGCAACCTAGATAACCTCAAAGG
CCTAGGGCAAGTATTAGAAAGCCAGTATCAGGTTATTCGTGTCGATGTGCCCAATCATGGTTTAAGTGAGCATTGGGATG
AGATGGATTACCCTCGCCTCGCCACGGCCATGGTTTCATTACTCGACGAACTGGCTATTGAACGCGCCCATATAGTGGGT
CACTCCATGGGCGGTAAAATCGCCATGGCGACAGCATTAGCCTTTCCAGAGCGCATTATCAGCATGGTGGCAGCAGATAT
TGCCCCCGTCGCCTATGAGCCAAGACATGACTTAGTGTTTGCCGCATTAGAAAGTCTGCCCTTAGAAGGTCATACCGACA
GACGCTTTGCCCTTAACCACTTAATAGACAAAGGTATCGACGAAGCCACGGCGCAGTTTTTACTCAAAAACCTGCAACGT
ACCGATACGGGCTTTCGTTGGAAGCTGAACTTAAGTGGTCTAAAGGCTTGTTACCCCAACATTATTGGTTGGCACAATCA
GGCGCCCAATTCAGTACAAAGCTATAATGGCCCGAGTCTATTTATTCGCGGCGGTGACTCAAATTATGTCACTAGCGAAC
ACAGAAGCGCTATCATGGCGCAATTCCCTGCAGCGCAGGCGAAAACCCTTGAAGGCTGTGGTCATTGGTTACATGCACAG
AAACCCGCCATCTTTAACCGGATTGTGTCTGAATTTATTGACAAACAAGCAATGTAA

Upstream 100 bases:

>100_bases
CGCCGCATACACGCCAAAGTAGCAACTGACTTGTCAAGCCAACGGCATTAAGGGCACAATTCGCGCCACGTTAAGCTAAA
CATATCCAACAGGAATCACC

Downstream 100 bases:

>100_bases
ATCAGCAGCAAACTCGACCTAAAGCATATGGCAGCCGCCATCTTGATGTGATATATTCGCGCCTCTTTTGGCCTTAGAAC
ACTCTCGAGGACTAAGAAAG

Product: alpha/beta hydrolase fold protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNFVSTGQGPAVLLIHGLFGNLDNLKGLGQVLESQYQVIRVDVPNHGLSEHWDEMDYPRLATAMVSLLDELAIERAHIVG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDLVFAALESLPLEGHTDRRFALNHLIDKGIDEATAQFLLKNLQR
TDTGFRWKLNLSGLKACYPNIIGWHNQAPNSVQSYNGPSLFIRGGDSNYVTSEHRSAIMAQFPAAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM

Sequences:

>Translated_258_residues
MNFVSTGQGPAVLLIHGLFGNLDNLKGLGQVLESQYQVIRVDVPNHGLSEHWDEMDYPRLATAMVSLLDELAIERAHIVG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDLVFAALESLPLEGHTDRRFALNHLIDKGIDEATAQFLLKNLQR
TDTGFRWKLNLSGLKACYPNIIGWHNQAPNSVQSYNGPSLFIRGGDSNYVTSEHRSAIMAQFPAAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM
>Mature_258_residues
MNFVSTGQGPAVLLIHGLFGNLDNLKGLGQVLESQYQVIRVDVPNHGLSEHWDEMDYPRLATAMVSLLDELAIERAHIVG
HSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDLVFAALESLPLEGHTDRRFALNHLIDKGIDEATAQFLLKNLQR
TDTGFRWKLNLSGLKACYPNIIGWHNQAPNSVQSYNGPSLFIRGGDSNYVTSEHRSAIMAQFPAAQAKTLEGCGHWLHAQ
KPAIFNRIVSEFIDKQAM

Specific function: Displays esterase activity toward palmitoyl-CoA, malonyl-CoA and pNP-butyrate [H]

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Homo sapiens, GI23200012, Length=252, Percent_Identity=35.7142857142857, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI23200008, Length=252, Percent_Identity=35.7142857142857, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI223941839, Length=246, Percent_Identity=31.3008130081301, Blast_Score=115, Evalue=3e-26,
Organism=Escherichia coli, GI1786902, Length=244, Percent_Identity=38.9344262295082, Blast_Score=175, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17508535, Length=254, Percent_Identity=32.6771653543307, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17507063, Length=258, Percent_Identity=25.5813953488372, Blast_Score=87, Evalue=7e-18,
Organism=Saccharomyces cerevisiae, GI6321468, Length=268, Percent_Identity=28.3582089552239, Blast_Score=96, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640348, Length=259, Percent_Identity=30.1158301158301, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24646055, Length=259, Percent_Identity=25.8687258687259, Blast_Score=78, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 28479; Mature: 28479

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFVSTGQGPAVLLIHGLFGNLDNLKGLGQVLESQYQVIRVDVPNHGLSEHWDEMDYPRL
CCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHH
ATAMVSLLDELAIERAHIVGHSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDLVF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AALESLPLEGHTDRRFALNHLIDKGIDEATAQFLLKNLQRTDTGFRWKLNLSGLKACYPN
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
IIGWHNQAPNSVQSYNGPSLFIRGGDSNYVTSEHRSAIMAQFPAAQAKTLEGCGHWLHAQ
HHCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KPAIFNRIVSEFIDKQAM
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFVSTGQGPAVLLIHGLFGNLDNLKGLGQVLESQYQVIRVDVPNHGLSEHWDEMDYPRL
CCCCCCCCCCEEEEEEHHHCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHCCCHHH
ATAMVSLLDELAIERAHIVGHSMGGKIAMATALAFPERIISMVAADIAPVAYEPRHDLVF
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
AALESLPLEGHTDRRFALNHLIDKGIDEATAQFLLKNLQRTDTGFRWKLNLSGLKACYPN
HHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHH
IIGWHNQAPNSVQSYNGPSLFIRGGDSNYVTSEHRSAIMAQFPAAQAKTLEGCGHWLHAQ
HHCCCCCCCCCHHCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCC
KPAIFNRIVSEFIDKQAM
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10493123 [H]